Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009
The response surface methodology and canonical analysis were employed to find the most suitable conditions for Lactobacillus helveticus to produce lactic acid from cheese whey in batch fermentation. The analyzed variables were temperature, pH, and the concentrations of lactose and yeast extract. The...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Chemical Engineering |
| Online Access: | http://dx.doi.org/10.1155/2012/303874 |
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| author | Marcelo Teixeira Leite Marcos Antonio de Souza Barrozo Eloizio Júlio Ribeiro |
| author_facet | Marcelo Teixeira Leite Marcos Antonio de Souza Barrozo Eloizio Júlio Ribeiro |
| author_sort | Marcelo Teixeira Leite |
| collection | DOAJ |
| description | The response surface methodology and canonical analysis were employed to find the most suitable conditions for Lactobacillus helveticus to produce lactic acid from cheese whey in batch fermentation. The analyzed variables were temperature, pH, and the concentrations of lactose and yeast extract. The experiments were carried out according to a central composite design with three center points. An empiric equation that correlated the concentration of lactic acid with the independent variables was proposed. The optimal conditions determined by the canonical analysis of the fitted model were 40°C, pH 6.8, 82 g/L of lactose, and 23.36 g/L of yeast extract. At this point, the lactic acid concentration reached 59.38 g/L. A subsequent fermentation, carried out under optimal conditions, confirmed the product concentration predicted by the adjusted model. This concentration of lactic acid is the highest ever reported for Lactobacillus helveticus ATCC 15009 in batch process using cheese whey as substrate. |
| format | Article |
| id | doaj-art-4d021d9a31cc4dd3b679b91c088eb9d2 |
| institution | Kabale University |
| issn | 1687-806X 1687-8078 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Chemical Engineering |
| spelling | doaj-art-4d021d9a31cc4dd3b679b91c088eb9d22025-02-03T01:30:32ZengWileyInternational Journal of Chemical Engineering1687-806X1687-80782012-01-01201210.1155/2012/303874303874Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009Marcelo Teixeira Leite0Marcos Antonio de Souza Barrozo1Eloizio Júlio Ribeiro2Center of Technology and Regional Development, Federal University of Paraíba, 58051-900 João Pessoa, PB, BrazilChemical Engineering College, Federal University of Uberlândia, 38408-100 Uberlândia, BrazilChemical Engineering College, Federal University of Uberlândia, 38408-100 Uberlândia, BrazilThe response surface methodology and canonical analysis were employed to find the most suitable conditions for Lactobacillus helveticus to produce lactic acid from cheese whey in batch fermentation. The analyzed variables were temperature, pH, and the concentrations of lactose and yeast extract. The experiments were carried out according to a central composite design with three center points. An empiric equation that correlated the concentration of lactic acid with the independent variables was proposed. The optimal conditions determined by the canonical analysis of the fitted model were 40°C, pH 6.8, 82 g/L of lactose, and 23.36 g/L of yeast extract. At this point, the lactic acid concentration reached 59.38 g/L. A subsequent fermentation, carried out under optimal conditions, confirmed the product concentration predicted by the adjusted model. This concentration of lactic acid is the highest ever reported for Lactobacillus helveticus ATCC 15009 in batch process using cheese whey as substrate.http://dx.doi.org/10.1155/2012/303874 |
| spellingShingle | Marcelo Teixeira Leite Marcos Antonio de Souza Barrozo Eloizio Júlio Ribeiro Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009 International Journal of Chemical Engineering |
| title | Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009 |
| title_full | Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009 |
| title_fullStr | Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009 |
| title_full_unstemmed | Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009 |
| title_short | Canonical Analysis Technique as an Approach to Determine Optimal Conditions for Lactic Acid Production by Lactobacillus helveticus ATCC 15009 |
| title_sort | canonical analysis technique as an approach to determine optimal conditions for lactic acid production by lactobacillus helveticus atcc 15009 |
| url | http://dx.doi.org/10.1155/2012/303874 |
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