Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway
Human and animals are continuously exposed to variable levels of mycotoxin that occur naturally in the diet. Aflatoxin B1 (AFB1) is the most toxic and carcinogenic aflatoxin. Probiotics have a vital role in mycotoxin detoxification. The objective of this study was to investigate potential protective...
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Elsevier
2025-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325010334 |
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| author | Juyu Wang Yu Zhao Yaxiong Zhou Kuan Wang Xiang Liu Jieyan Yang Limei Zhang Weijie Qu Hongjiang Wei Xiaolong Gu |
| author_facet | Juyu Wang Yu Zhao Yaxiong Zhou Kuan Wang Xiang Liu Jieyan Yang Limei Zhang Weijie Qu Hongjiang Wei Xiaolong Gu |
| author_sort | Juyu Wang |
| collection | DOAJ |
| description | Human and animals are continuously exposed to variable levels of mycotoxin that occur naturally in the diet. Aflatoxin B1 (AFB1) is the most toxic and carcinogenic aflatoxin. Probiotics have a vital role in mycotoxin detoxification. The objective of this study was to investigate potential protective effects of probiotics Bacillus amyloliquefaciens and its mechanism. In in vitro experiments, exposure of AML-12 cells to AFB1 reduced cell viability, increased oxidative stress, and induced cell pyroptosis. Additionally, there was down-regulation of Nuclear factor erythropoietin-2-related factor 2/heme oxygenase 1 (Nrf2/HO-1) expression. Importantly, Nrf2siRNA exacerbated AFB1-induced cell pyroptosis, whereas overexpression of Nrf2 alleviated it. Furthermore, Bacillus amyloliquefaciens reversed the upregulated cell pyroptosis, and downregulated Nrf2/HO-1 induced by AFB1. Interestingly, Nrf2 siRNA abolished the protective effect exerted by Bacillus amyloliquefaciens, whereas overexpression of Nrf2 had the opposite effect. In in vivo experiments, AFB1 caused liver impairment, oxidative stress and cell pyroptosis, whereas Bacillus amyloliquefaciens exerts its protective effect via Nrf2/HO-1 signaling in AFB1-induced deleterious effects. In summary, Bacillus amyloliquefaciens may regulate the Nrf2/HO-1 signaling pathway to mitigate AFB1-induced cell pyroptosis, thereby providing a potential therapeutic intervention for mycotoxicosis associated with AFB1-induced hepatic injury. |
| format | Article |
| id | doaj-art-4cefa864cbbd4a55a5ee5f5906f9727e |
| institution | DOAJ |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-4cefa864cbbd4a55a5ee5f5906f9727e2025-08-20T03:03:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211868810.1016/j.ecoenv.2025.118688Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathwayJuyu Wang0Yu Zhao1Yaxiong Zhou2Kuan Wang3Xiang Liu4Jieyan Yang5Limei Zhang6Weijie Qu7Hongjiang Wei8Xiaolong Gu9Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, ChinaKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Kunming 650201, China; Xenotransplantation Engineering Research Center in Yunnan Province, Kunming 650201, China; Corresponding author at: Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Kunming 650201, China.Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.95, Panlong District, Kunming 650051, China; Corresponding author.Human and animals are continuously exposed to variable levels of mycotoxin that occur naturally in the diet. Aflatoxin B1 (AFB1) is the most toxic and carcinogenic aflatoxin. Probiotics have a vital role in mycotoxin detoxification. The objective of this study was to investigate potential protective effects of probiotics Bacillus amyloliquefaciens and its mechanism. In in vitro experiments, exposure of AML-12 cells to AFB1 reduced cell viability, increased oxidative stress, and induced cell pyroptosis. Additionally, there was down-regulation of Nuclear factor erythropoietin-2-related factor 2/heme oxygenase 1 (Nrf2/HO-1) expression. Importantly, Nrf2siRNA exacerbated AFB1-induced cell pyroptosis, whereas overexpression of Nrf2 alleviated it. Furthermore, Bacillus amyloliquefaciens reversed the upregulated cell pyroptosis, and downregulated Nrf2/HO-1 induced by AFB1. Interestingly, Nrf2 siRNA abolished the protective effect exerted by Bacillus amyloliquefaciens, whereas overexpression of Nrf2 had the opposite effect. In in vivo experiments, AFB1 caused liver impairment, oxidative stress and cell pyroptosis, whereas Bacillus amyloliquefaciens exerts its protective effect via Nrf2/HO-1 signaling in AFB1-induced deleterious effects. In summary, Bacillus amyloliquefaciens may regulate the Nrf2/HO-1 signaling pathway to mitigate AFB1-induced cell pyroptosis, thereby providing a potential therapeutic intervention for mycotoxicosis associated with AFB1-induced hepatic injury.http://www.sciencedirect.com/science/article/pii/S0147651325010334Aflatoxin B1Bacillus amyloliquefaciensNrf2/HO-1 signaling |
| spellingShingle | Juyu Wang Yu Zhao Yaxiong Zhou Kuan Wang Xiang Liu Jieyan Yang Limei Zhang Weijie Qu Hongjiang Wei Xiaolong Gu Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway Ecotoxicology and Environmental Safety Aflatoxin B1 Bacillus amyloliquefaciens Nrf2/HO-1 signaling |
| title | Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway |
| title_full | Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway |
| title_fullStr | Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway |
| title_full_unstemmed | Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway |
| title_short | Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway |
| title_sort | inhibition of aflatoxin b1 induced murine hepatocyte pyroptosis by bacillus amyloliquefaciens by activation of the nrf2 ho 1 pathway |
| topic | Aflatoxin B1 Bacillus amyloliquefaciens Nrf2/HO-1 signaling |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325010334 |
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