Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes
Abstract Functional ultrasound imaging (fUS) and ultrasound localization microscopy (ULM) are advanced ultrasound imaging modalities for assessing both functional and anatomical characteristics of the brain. However, the application of these techniques at a whole-brain scale has been limited by tech...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-96647-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145509895831552 |
|---|---|
| author | Mathis Vert Ge Zhang Adrien Bertolo Nathalie Ialy-Radio Sophie Pezet Bruno Osmanski Thomas Deffieux Mohamed Nouhoum Mickael Tanter |
| author_facet | Mathis Vert Ge Zhang Adrien Bertolo Nathalie Ialy-Radio Sophie Pezet Bruno Osmanski Thomas Deffieux Mohamed Nouhoum Mickael Tanter |
| author_sort | Mathis Vert |
| collection | DOAJ |
| description | Abstract Functional ultrasound imaging (fUS) and ultrasound localization microscopy (ULM) are advanced ultrasound imaging modalities for assessing both functional and anatomical characteristics of the brain. However, the application of these techniques at a whole-brain scale has been limited by technological challenges. While conventional linear acoustic probes provide a narrow 2D field of view and matrix probes lack sufficient sensitivity for 3D transcranial fUS, multi-array probes have been developed to combine high sensitivity to blood flow with fast 3D acquisitions. In this study, we present a novel approach for the combined implementation of transcranial whole-brain fUS and ULM in mice using a motorized multi-array probe. This technique provides high-resolution, non-invasive imaging of neurovascular dynamics across the entire brain. Our findings reveal a significant correlation between absolute cerebral blood volume (ΔCBV) increases and microbubble speed, indicating vessel-level dependency of the evoked response. However, the lack of correlation with relative CBV (rCBV) suggests that fUS cannot distinguish functional responses alterations across different arterial vascular compartments. This methodology holds promise for advancing our understanding of neurovascular coupling and could be applied in brain disease diagnostics and therapeutic monitoring. |
| format | Article |
| id | doaj-art-4cc42e1114c44b2fbcb97e97a18c13aa |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4cc42e1114c44b2fbcb97e97a18c13aa2025-08-20T02:28:05ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-96647-7Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probesMathis Vert0Ge Zhang1Adrien Bertolo2Nathalie Ialy-Radio3Sophie Pezet4Bruno Osmanski5Thomas Deffieux6Mohamed Nouhoum7Mickael Tanter8Physics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSPhysics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSPhysics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSPhysics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSPhysics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSIconeusPhysics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSIconeusPhysics for Medicine Paris, INSERM U1273, ESPCI Paris, PSL University, CNRSAbstract Functional ultrasound imaging (fUS) and ultrasound localization microscopy (ULM) are advanced ultrasound imaging modalities for assessing both functional and anatomical characteristics of the brain. However, the application of these techniques at a whole-brain scale has been limited by technological challenges. While conventional linear acoustic probes provide a narrow 2D field of view and matrix probes lack sufficient sensitivity for 3D transcranial fUS, multi-array probes have been developed to combine high sensitivity to blood flow with fast 3D acquisitions. In this study, we present a novel approach for the combined implementation of transcranial whole-brain fUS and ULM in mice using a motorized multi-array probe. This technique provides high-resolution, non-invasive imaging of neurovascular dynamics across the entire brain. Our findings reveal a significant correlation between absolute cerebral blood volume (ΔCBV) increases and microbubble speed, indicating vessel-level dependency of the evoked response. However, the lack of correlation with relative CBV (rCBV) suggests that fUS cannot distinguish functional responses alterations across different arterial vascular compartments. This methodology holds promise for advancing our understanding of neurovascular coupling and could be applied in brain disease diagnostics and therapeutic monitoring.https://doi.org/10.1038/s41598-025-96647-7Functional ultrasound imagingUltrasound localization microscopyMulti-array probeCerebral blood volumeBrain imaging |
| spellingShingle | Mathis Vert Ge Zhang Adrien Bertolo Nathalie Ialy-Radio Sophie Pezet Bruno Osmanski Thomas Deffieux Mohamed Nouhoum Mickael Tanter Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes Scientific Reports Functional ultrasound imaging Ultrasound localization microscopy Multi-array probe Cerebral blood volume Brain imaging |
| title | Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes |
| title_full | Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes |
| title_fullStr | Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes |
| title_full_unstemmed | Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes |
| title_short | Transcranial brain-wide functional ultrasound and ultrasound localization microscopy in mice using multi-array probes |
| title_sort | transcranial brain wide functional ultrasound and ultrasound localization microscopy in mice using multi array probes |
| topic | Functional ultrasound imaging Ultrasound localization microscopy Multi-array probe Cerebral blood volume Brain imaging |
| url | https://doi.org/10.1038/s41598-025-96647-7 |
| work_keys_str_mv | AT mathisvert transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT gezhang transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT adrienbertolo transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT nathalieialyradio transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT sophiepezet transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT brunoosmanski transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT thomasdeffieux transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT mohamednouhoum transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes AT mickaeltanter transcranialbrainwidefunctionalultrasoundandultrasoundlocalizationmicroscopyinmiceusingmultiarrayprobes |