Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar
BackgroundGrowing reports are dedicated to providing novel agents for wound healing with fewer adverse effects and higher efficacy. The efficacy of nanofibers composed of polyvinyl alcohol (PVA)/polyethylene oxide (PEO)/chitosan (CS) in promoting wound healing can be attributed to their ability to s...
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Frontiers Media S.A.
2025-01-01
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| author | Leila Almasi Elham Arkan Mohammad Hosein Farzaei Amin Iranpanah Cyrus Jalili Fatemeh Abbaszadeh Faranak Aghaz Sajad Fakhri Javier Echeverría |
| author_facet | Leila Almasi Elham Arkan Mohammad Hosein Farzaei Amin Iranpanah Cyrus Jalili Fatemeh Abbaszadeh Faranak Aghaz Sajad Fakhri Javier Echeverría |
| author_sort | Leila Almasi |
| collection | DOAJ |
| description | BackgroundGrowing reports are dedicated to providing novel agents for wound healing with fewer adverse effects and higher efficacy. The efficacy of nanofibers composed of polyvinyl alcohol (PVA)/polyethylene oxide (PEO)/chitosan (CS) in promoting wound healing can be attributed to their ability to stimulate collagen production. Among the herbal agents with fewer adverse effects, Tragopogon graminifolius DC. [Asteraceae] (TG), also called “Sheng” in traditional Iranian medicine, is one of the most efficacious plants for treating various skin injuries due to its several pharmacological and biological effects like anti-inflammatory and antioxidant properties.PurposeIn the present study, our objective was to assess the wound-healing activity of PVA/PEO/CS nanofibers containing TG in a rat model of excision wound repair.MethodsSynthesized nanofibers from PVA, PEO, and CS were done by the electrospinning method and confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR). The release tests of nanofibers were assessed through the UV-visible method at different time intervals, which were conducted for about 60 h. To evaluate the wound healing effects, rats were divided into four distinct groups, including negative control (untreated), phenytoin cream (as positive control), polymer (PVA/PEO/CS), and drug (nanofiber-containing 50% of TG extract; named PVA/PEO/CS/TG) groups. All treatments were administered topically once daily for 14 days. Wound size changes were investigated in different time intervals. On the 15th day, nitrite and catalase serum levels were measured. Furthermore, samples of skin tissue were extracted and subjected to histopathological analysis.ResultsPVA/PEO/CS nanofibers containing 1.2 g of PVA, 0.3 g of PEO, and 0.8 g of CS, along with 50% of TG extract (PVA/PEO/CS/TG) at 17 kV were selected based on its favorable morphology and uniform quality. PVA/PEO/CS/TG represented a notable reduction in wound sizes. Moreover, in histopathological analysis, PVA/PEO/CS/TG showed a lower presence of inflammatory cells, higher density of dermis collagen fibers, and better regeneration of the epidemic layer. In addition, PVA/PEO/CS/TG elevated plasma antioxidant capacity via increasing catalase while reducing nitrite levels.ConclusionPVA/PEO/CS/TG is a promising wound dressing nanofiber with antioxidant and tissue regeneration potential. These results encourage further studies for the development of TG nanofibers as promising agents in treating and accelerating the process of excision wound repair. |
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| id | doaj-art-4caf1c201660458bb1b262becfbc6a8c |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-4caf1c201660458bb1b262becfbc6a8c2025-01-31T06:40:08ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011610.3389/fphar.2025.15330101533010Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scarLeila Almasi0Elham Arkan1Mohammad Hosein Farzaei2Amin Iranpanah3Cyrus Jalili4Fatemeh Abbaszadeh5Faranak Aghaz6Sajad Fakhri7Javier Echeverría8Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranNano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IranPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IranMedical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranNeurobiology Research Center, Institute of Neuroscience and Cognition, Shahid Beheshti University of Medical Sciences, Tehran, IranNano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IranDepartamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileBackgroundGrowing reports are dedicated to providing novel agents for wound healing with fewer adverse effects and higher efficacy. The efficacy of nanofibers composed of polyvinyl alcohol (PVA)/polyethylene oxide (PEO)/chitosan (CS) in promoting wound healing can be attributed to their ability to stimulate collagen production. Among the herbal agents with fewer adverse effects, Tragopogon graminifolius DC. [Asteraceae] (TG), also called “Sheng” in traditional Iranian medicine, is one of the most efficacious plants for treating various skin injuries due to its several pharmacological and biological effects like anti-inflammatory and antioxidant properties.PurposeIn the present study, our objective was to assess the wound-healing activity of PVA/PEO/CS nanofibers containing TG in a rat model of excision wound repair.MethodsSynthesized nanofibers from PVA, PEO, and CS were done by the electrospinning method and confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR). The release tests of nanofibers were assessed through the UV-visible method at different time intervals, which were conducted for about 60 h. To evaluate the wound healing effects, rats were divided into four distinct groups, including negative control (untreated), phenytoin cream (as positive control), polymer (PVA/PEO/CS), and drug (nanofiber-containing 50% of TG extract; named PVA/PEO/CS/TG) groups. All treatments were administered topically once daily for 14 days. Wound size changes were investigated in different time intervals. On the 15th day, nitrite and catalase serum levels were measured. Furthermore, samples of skin tissue were extracted and subjected to histopathological analysis.ResultsPVA/PEO/CS nanofibers containing 1.2 g of PVA, 0.3 g of PEO, and 0.8 g of CS, along with 50% of TG extract (PVA/PEO/CS/TG) at 17 kV were selected based on its favorable morphology and uniform quality. PVA/PEO/CS/TG represented a notable reduction in wound sizes. Moreover, in histopathological analysis, PVA/PEO/CS/TG showed a lower presence of inflammatory cells, higher density of dermis collagen fibers, and better regeneration of the epidemic layer. In addition, PVA/PEO/CS/TG elevated plasma antioxidant capacity via increasing catalase while reducing nitrite levels.ConclusionPVA/PEO/CS/TG is a promising wound dressing nanofiber with antioxidant and tissue regeneration potential. These results encourage further studies for the development of TG nanofibers as promising agents in treating and accelerating the process of excision wound repair.https://www.frontiersin.org/articles/10.3389/fphar.2025.1533010/fullwoundskin scarTragopogon graminifolius DC.nanofibersantioxidantelectrospinning |
| spellingShingle | Leila Almasi Elham Arkan Mohammad Hosein Farzaei Amin Iranpanah Cyrus Jalili Fatemeh Abbaszadeh Faranak Aghaz Sajad Fakhri Javier Echeverría Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar Frontiers in Pharmacology wound skin scar Tragopogon graminifolius DC. nanofibers antioxidant electrospinning |
| title | Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar |
| title_full | Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar |
| title_fullStr | Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar |
| title_full_unstemmed | Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar |
| title_short | Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar |
| title_sort | preparation of tragopogon graminifolius loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar |
| topic | wound skin scar Tragopogon graminifolius DC. nanofibers antioxidant electrospinning |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1533010/full |
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