M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury
LncRNA is a major factor in the occurrence and development of many diseases. However, its mechanism in cerebral ischemia/reperfusion injury (CIRI) is yet unknown. In this study, the transcriptional level and methylation modification level of LncRNAs before and after mechanical thrombectomy were comp...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2024/4596974 |
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| author | Jierong Mo Zhiquan Li Zhengfei Yang Zuhua Huang Pengpeng Guo Jianfeng Gao Haiqiong xiao Ping Ye Haini Qin Tianen Zhou Jun Jiang |
| author_facet | Jierong Mo Zhiquan Li Zhengfei Yang Zuhua Huang Pengpeng Guo Jianfeng Gao Haiqiong xiao Ping Ye Haini Qin Tianen Zhou Jun Jiang |
| author_sort | Jierong Mo |
| collection | DOAJ |
| description | LncRNA is a major factor in the occurrence and development of many diseases. However, its mechanism in cerebral ischemia/reperfusion injury (CIRI) is yet unknown. In this study, the transcriptional level and methylation modification level of LncRNAs before and after mechanical thrombectomy were compared by high-throughput sequencing. Venn diagram, Spearman correlation analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, TargetScan, and miRanda were used to analyze the experimental data. The results showed that four key LncRNAs changed at both transcription and methylation levels. Specifically, LncRNA FAR2, LINC02431, and AL357060.1 were downregulated and hypomethylated, while LncRNA FOXD2-AS1 was upregulated and hypomethylated. Moreover, positive regulation of angiogenesis, protein domain–specific binding, autophagy pathway, PPAR signaling pathway, and MAPK signaling pathway were co-enriched between LncRNAs with different expression levels and different methylation levels. Finally, a LncRNA-miRNA-mRNA network was constructed. Therefore, this study explored the potential key LncRNAs and regulatory mechanisms of CIRI. |
| format | Article |
| id | doaj-art-4c8f70b123b3480cb159076433e30757 |
| institution | Kabale University |
| issn | 2314-4378 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4c8f70b123b3480cb159076433e307572025-02-03T11:09:32ZengWileyInternational Journal of Genomics2314-43782024-01-01202410.1155/2024/4596974M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion InjuryJierong Mo0Zhiquan Li1Zhengfei Yang2Zuhua Huang3Pengpeng Guo4Jianfeng Gao5Haiqiong xiao6Ping Ye7Haini Qin8Tianen Zhou9Jun Jiang10Department of EmergencyGuangdong Medical UniversitySun Yat-Sen Memorial HospitalDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyDepartment of EmergencyLncRNA is a major factor in the occurrence and development of many diseases. However, its mechanism in cerebral ischemia/reperfusion injury (CIRI) is yet unknown. In this study, the transcriptional level and methylation modification level of LncRNAs before and after mechanical thrombectomy were compared by high-throughput sequencing. Venn diagram, Spearman correlation analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, TargetScan, and miRanda were used to analyze the experimental data. The results showed that four key LncRNAs changed at both transcription and methylation levels. Specifically, LncRNA FAR2, LINC02431, and AL357060.1 were downregulated and hypomethylated, while LncRNA FOXD2-AS1 was upregulated and hypomethylated. Moreover, positive regulation of angiogenesis, protein domain–specific binding, autophagy pathway, PPAR signaling pathway, and MAPK signaling pathway were co-enriched between LncRNAs with different expression levels and different methylation levels. Finally, a LncRNA-miRNA-mRNA network was constructed. Therefore, this study explored the potential key LncRNAs and regulatory mechanisms of CIRI.http://dx.doi.org/10.1155/2024/4596974 |
| spellingShingle | Jierong Mo Zhiquan Li Zhengfei Yang Zuhua Huang Pengpeng Guo Jianfeng Gao Haiqiong xiao Ping Ye Haini Qin Tianen Zhou Jun Jiang M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury International Journal of Genomics |
| title | M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury |
| title_full | M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury |
| title_fullStr | M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury |
| title_full_unstemmed | M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury |
| title_short | M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury |
| title_sort | m6a modification and transcription analysis of lncrna in cerebral ischemia reperfusion injury |
| url | http://dx.doi.org/10.1155/2024/4596974 |
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