Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells
This study was aimed at investigating the effects of placenta-derived mesenchymal stem cells (PL-MSCs) on particulate matter- (PM-) exposed human middle ear epithelial cells (HMEECs). HMEECs were treated with 300 μg/ml PM for 24 hours. The PL-MSCs were cocultured with PM-treated HMEECs. Cells were h...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/4357684 |
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| author | So Young Kim Seung Ha Oh Jun Ho Lee Myung-Whan Suh Moo Kyun Park |
| author_facet | So Young Kim Seung Ha Oh Jun Ho Lee Myung-Whan Suh Moo Kyun Park |
| author_sort | So Young Kim |
| collection | DOAJ |
| description | This study was aimed at investigating the effects of placenta-derived mesenchymal stem cells (PL-MSCs) on particulate matter- (PM-) exposed human middle ear epithelial cells (HMEECs). HMEECs were treated with 300 μg/ml PM for 24 hours. The PL-MSCs were cocultured with PM-treated HMEECs. Cells were harvested on days 0, 1, and 4, and the expression of the inflammatory genes TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs and anti-inflammatory genes PTGES, TGFβ, and VEGF in PL-MSCs was examined by qRT-PCR. The culture media were collected to measure the secreted PGE2 level using an enzyme-linked immunosorbent assay. The mRNA expression of TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs increased following PM treatment. PM-treated HMEECs cocultured with PL-MSCs showed alleviated inflammatory reactions represented by lower mRNA expression levels of MUC5B, TNFα, IL1β, and IL6 compared to monocultured PM-treated HMEECs. The mRNA expression levels of PGE2, TGFβ, and VEGF were elevated in cocultured PL-MSCs compared to those of control PL-MSCs. The medium of PM-treated HMEECs cocultured with PL-MSCs exhibited increased PGE2 levels. The increased inflammatory response in PM-treated HMEECs was reversed using PL-MSCs. The PGE2, TGFβ, and VEGF were the mediators of the anti-inflammatory effects of PL-MSCs. |
| format | Article |
| id | doaj-art-4c8da1debf8a4b9fa2df9a191274b56d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-4c8da1debf8a4b9fa2df9a191274b56d2025-02-03T01:01:21ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/43576844357684Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial CellsSo Young Kim0Seung Ha Oh1Jun Ho Lee2Myung-Whan Suh3Moo Kyun Park4Department of Otorhinolaryngology-Head & Neck Surgery, CHA University College of Medicine, Republic of KoreaDepartment of Otorhinolaryngology-Head & Neck Surgery, Seoul National University College of Medicine, 101 Daehak-Ro Jongno-Gu, Seoul 110-744, Republic of KoreaDepartment of Otorhinolaryngology-Head & Neck Surgery, Seoul National University College of Medicine, 101 Daehak-Ro Jongno-Gu, Seoul 110-744, Republic of KoreaDepartment of Otorhinolaryngology-Head & Neck Surgery, Seoul National University College of Medicine, 101 Daehak-Ro Jongno-Gu, Seoul 110-744, Republic of KoreaDepartment of Otorhinolaryngology-Head & Neck Surgery, Seoul National University College of Medicine, 101 Daehak-Ro Jongno-Gu, Seoul 110-744, Republic of KoreaThis study was aimed at investigating the effects of placenta-derived mesenchymal stem cells (PL-MSCs) on particulate matter- (PM-) exposed human middle ear epithelial cells (HMEECs). HMEECs were treated with 300 μg/ml PM for 24 hours. The PL-MSCs were cocultured with PM-treated HMEECs. Cells were harvested on days 0, 1, and 4, and the expression of the inflammatory genes TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs and anti-inflammatory genes PTGES, TGFβ, and VEGF in PL-MSCs was examined by qRT-PCR. The culture media were collected to measure the secreted PGE2 level using an enzyme-linked immunosorbent assay. The mRNA expression of TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs increased following PM treatment. PM-treated HMEECs cocultured with PL-MSCs showed alleviated inflammatory reactions represented by lower mRNA expression levels of MUC5B, TNFα, IL1β, and IL6 compared to monocultured PM-treated HMEECs. The mRNA expression levels of PGE2, TGFβ, and VEGF were elevated in cocultured PL-MSCs compared to those of control PL-MSCs. The medium of PM-treated HMEECs cocultured with PL-MSCs exhibited increased PGE2 levels. The increased inflammatory response in PM-treated HMEECs was reversed using PL-MSCs. The PGE2, TGFβ, and VEGF were the mediators of the anti-inflammatory effects of PL-MSCs.http://dx.doi.org/10.1155/2019/4357684 |
| spellingShingle | So Young Kim Seung Ha Oh Jun Ho Lee Myung-Whan Suh Moo Kyun Park Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells Stem Cells International |
| title | Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells |
| title_full | Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells |
| title_fullStr | Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells |
| title_full_unstemmed | Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells |
| title_short | Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells |
| title_sort | effects of placenta derived mesenchymal stem cells on the particulate matter induced damages in human middle ear epithelial cells |
| url | http://dx.doi.org/10.1155/2019/4357684 |
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