Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing
Objective: This study aimed to investigate whether platelet-rich plasma (PRP) obtained from the blood of rats preconditioned with hyperbaric oxygen (HBOP) would enhance the biological activity of PRP and accelerate the healing process of femur fractures in a rat model. Design: PRP was derived from b...
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Elsevier
2025-03-01
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| Series: | Journal of Orthopaedic Translation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X24001682 |
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| author | Wen-Shuo Chang Chien-Cheng Huang Tzu-Hao Chen Ssu-Han Chao Cheng-Hsien Lin Ching-Ping Chang Chi-Sheng Chien |
| author_facet | Wen-Shuo Chang Chien-Cheng Huang Tzu-Hao Chen Ssu-Han Chao Cheng-Hsien Lin Ching-Ping Chang Chi-Sheng Chien |
| author_sort | Wen-Shuo Chang |
| collection | DOAJ |
| description | Objective: This study aimed to investigate whether platelet-rich plasma (PRP) obtained from the blood of rats preconditioned with hyperbaric oxygen (HBOP) would enhance the biological activity of PRP and accelerate the healing process of femur fractures in a rat model. Design: PRP was derived from blood samples of healthy rats subjected to either hyperbaric oxygen (hPRP) or normobaric air (nPRP). A closed femur fracture model was established in male Wistar rats, with treatments of hPRP or nPRP administered around the fracture site immediately post-fracture and on days 7, 14, 21, and 28. Growth factor concentrations in hPRP and nPRP were biochemically quantified. Bone healing was assessed weekly by X-ray, while histological and immunofluorescence analyses evaluated inflammatory status, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) expression, and the presence of osteoblasts, osteoclasts, and osteocytes during healing. The effects of hPRP and nPRP on MC3T3-E1 preosteoblast migration and proliferation were also tested in vitro. Results: hPRP showed significantly higher concentrations of growth factors such as activin-A, brain-derived neurotrophic factor, nerve growth factor, Flt-3 Ligand, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, and platelet-derived growth factor, compared to nPRP. In vitro, hPRP demonstrated more significant effects on preosteoblast migration and proliferation. In vivo, hPRP treatment resulted in enhanced bone healing, higher OPG levels in osteoblasts and osteoclasts, and an elevated OPG/RANKL ratio compared to nPRP. Conclusions: HBOP enhances the biological activity of PRP and accelerates bone healing in a closed femur fracture model in rats. This study highlights the regenerative potential of PRP when preconditioned with hyperbaric oxygen for use in bone fracture therapy. Significance statement: PRP is widely used in treating bone defects and fractures, but its enhancement through HBOP remains underexplored. Our findings demonstrate that HBOP potentiates the biological activity of PRP, offering promising therapeutic potential for bone fracture healing. The translational potential of this article: Enriching growth factors in PRP through HBOP could significantly improve tissue regeneration, especially in bone healing. The potential of hPRP in clinical applications is highly promising, particularly in orthopaedic surgery, trauma care, sports medicine, and managing bone healing in compromised patients. |
| format | Article |
| id | doaj-art-4c702437e3494d1aae61320917a45cd1 |
| institution | Kabale University |
| issn | 2214-031X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj-art-4c702437e3494d1aae61320917a45cd12025-01-20T04:17:27ZengElsevierJournal of Orthopaedic Translation2214-031X2025-03-0151112Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healingWen-Shuo Chang0Chien-Cheng Huang1Tzu-Hao Chen2Ssu-Han Chao3Cheng-Hsien Lin4Ching-Ping Chang5Chi-Sheng Chien6Department of Orthopedics, Chi Mei Medical Center, Tainan, TaiwanDepartment of Emergency Medicine, Chi Mei Medical Center, Tainan, Taiwan; School of Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan; Department of Emergency Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanDepartment of Medical Research, Chi Mei Medical Center, Tainan, TaiwanDepartment of Medical Research, Chi Mei Medical Center, Tainan, TaiwanDepartment of Medicine, MacKay Medical College, New Taipei City, TaiwanDepartment of Medical Research, Chi Mei Medical Center, Tainan, Taiwan; Corresponding author. Department of Medical Research, Chi Mei Medical Center, No. 901, Zhonghua Rd., Yongkang District, Tainan City, 710, Taiwan.Department of Orthopedics, Chi Mei Medical Center, Tainan, Taiwan; Corresponding author. Department of Orthopedics, Chi Mei Medical Center, No. 901, Zhonghua Rd., Yongkang District, Tainan City, 710, Taiwan.Objective: This study aimed to investigate whether platelet-rich plasma (PRP) obtained from the blood of rats preconditioned with hyperbaric oxygen (HBOP) would enhance the biological activity of PRP and accelerate the healing process of femur fractures in a rat model. Design: PRP was derived from blood samples of healthy rats subjected to either hyperbaric oxygen (hPRP) or normobaric air (nPRP). A closed femur fracture model was established in male Wistar rats, with treatments of hPRP or nPRP administered around the fracture site immediately post-fracture and on days 7, 14, 21, and 28. Growth factor concentrations in hPRP and nPRP were biochemically quantified. Bone healing was assessed weekly by X-ray, while histological and immunofluorescence analyses evaluated inflammatory status, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) expression, and the presence of osteoblasts, osteoclasts, and osteocytes during healing. The effects of hPRP and nPRP on MC3T3-E1 preosteoblast migration and proliferation were also tested in vitro. Results: hPRP showed significantly higher concentrations of growth factors such as activin-A, brain-derived neurotrophic factor, nerve growth factor, Flt-3 Ligand, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, and platelet-derived growth factor, compared to nPRP. In vitro, hPRP demonstrated more significant effects on preosteoblast migration and proliferation. In vivo, hPRP treatment resulted in enhanced bone healing, higher OPG levels in osteoblasts and osteoclasts, and an elevated OPG/RANKL ratio compared to nPRP. Conclusions: HBOP enhances the biological activity of PRP and accelerates bone healing in a closed femur fracture model in rats. This study highlights the regenerative potential of PRP when preconditioned with hyperbaric oxygen for use in bone fracture therapy. Significance statement: PRP is widely used in treating bone defects and fractures, but its enhancement through HBOP remains underexplored. Our findings demonstrate that HBOP potentiates the biological activity of PRP, offering promising therapeutic potential for bone fracture healing. The translational potential of this article: Enriching growth factors in PRP through HBOP could significantly improve tissue regeneration, especially in bone healing. The potential of hPRP in clinical applications is highly promising, particularly in orthopaedic surgery, trauma care, sports medicine, and managing bone healing in compromised patients.http://www.sciencedirect.com/science/article/pii/S2214031X24001682Bone healingGrowth factorHyperbaric oxygenOsteogenesisPlatelet-rich plasma |
| spellingShingle | Wen-Shuo Chang Chien-Cheng Huang Tzu-Hao Chen Ssu-Han Chao Cheng-Hsien Lin Ching-Ping Chang Chi-Sheng Chien Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing Journal of Orthopaedic Translation Bone healing Growth factor Hyperbaric oxygen Osteogenesis Platelet-rich plasma |
| title | Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing |
| title_full | Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing |
| title_fullStr | Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing |
| title_full_unstemmed | Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing |
| title_short | Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing |
| title_sort | hyperbaric oxygen potentiates platelet rich plasma composition and accelerates bone healing |
| topic | Bone healing Growth factor Hyperbaric oxygen Osteogenesis Platelet-rich plasma |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X24001682 |
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