Genome-wide association studies with prolapsed gland of the third eyelid in dogs
Cherry eye, the common name for the prolapse of the third eyelid gland in dogs, is a widespread ophthalmic disease affecting dogs of various breeds. This condition severely affects the quality of life of affected dogs, and its underlying cause remains unresolved. In this study, 170K SNP microarray d...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1520155/full |
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| author | Yu Zeng Yu Zeng Yu Zeng Cundong Feng Zheli Jiang Zheli Jiang Zheli Jiang Weian Du Shan He Shan He Shan He Xingnuan Li Xingnuan Li Xingnuan Li Yi Fan Yi Fan Yi Fan Xiao Ouyang Xiao Ouyang Xiao Ouyang Bixin Huang Bixin Huang Bixin Huang Yan Su Yan Su Yan Su Siyu Wang Siyu Wang Siyu Wang Rongxing Wei Zonghao Dai Peng Jin Jianyun Liu Jianyun Liu Jianyun Liu Qianyong Yang Qianyong Yang Qianyong Yang |
| author_facet | Yu Zeng Yu Zeng Yu Zeng Cundong Feng Zheli Jiang Zheli Jiang Zheli Jiang Weian Du Shan He Shan He Shan He Xingnuan Li Xingnuan Li Xingnuan Li Yi Fan Yi Fan Yi Fan Xiao Ouyang Xiao Ouyang Xiao Ouyang Bixin Huang Bixin Huang Bixin Huang Yan Su Yan Su Yan Su Siyu Wang Siyu Wang Siyu Wang Rongxing Wei Zonghao Dai Peng Jin Jianyun Liu Jianyun Liu Jianyun Liu Qianyong Yang Qianyong Yang Qianyong Yang |
| author_sort | Yu Zeng |
| collection | DOAJ |
| description | Cherry eye, the common name for the prolapse of the third eyelid gland in dogs, is a widespread ophthalmic disease affecting dogs of various breeds. This condition severely affects the quality of life of affected dogs, and its underlying cause remains unresolved. In this study, 170K SNP microarray data were collected from 653 brachycephalic dogs and 788 brachycephalic and mesocephalic dogs. These two datasets were analyzed separately in genome-wide association studies (GWAS) involving 12 dog breeds affected by cherry eye. The GWAS analysis of 653 short-headed dogs revealed that four SNPs in the CFA3:15627075-15983629 bp region exceeded the genome-level significance threshold. Association analysis of this region also indicated that these four SNPs were strongly associated. Gene annotation showed that the region contained genes such as KIAA0825, FAM172A, and NR2F1, of which NR2F1 was associated with eye development. The results showed that GWAS analysis performed on 788 short- and medium-headed dogs identified five SNPs in the CFA22:15627075-15983629 bp region that exceeded the genome-level significance threshold, and association analysis was performed in this region, which showed that these five SNPs were strongly associated. In addition, 104 annotated genes were identified in both GWAS. To explore the genes involved in cherry eyes, we performed GO functional enrichment analysis. The genes involved in the high pathway were DIO3 and TTC8. In addition, an in-depth analysis revealed 33 genes associated with eye development and diseases. Our study provides new perspectives for further understanding cherry eye in dogs. |
| format | Article |
| id | doaj-art-4c5d3462825241489033e48a1450b8bc |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-4c5d3462825241489033e48a1450b8bc2025-01-24T14:46:00ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15201551520155Genome-wide association studies with prolapsed gland of the third eyelid in dogsYu Zeng0Yu Zeng1Yu Zeng2Cundong Feng3Zheli Jiang4Zheli Jiang5Zheli Jiang6Weian Du7Shan He8Shan He9Shan He10Xingnuan Li11Xingnuan Li12Xingnuan Li13Yi Fan14Yi Fan15Yi Fan16Xiao Ouyang17Xiao Ouyang18Xiao Ouyang19Bixin Huang20Bixin Huang21Bixin Huang22Yan Su23Yan Su24Yan Su25Siyu Wang26Siyu Wang27Siyu Wang28Rongxing Wei29Zonghao Dai30Peng Jin31Jianyun Liu32Jianyun Liu33Jianyun Liu34Qianyong Yang35Qianyong Yang36Qianyong Yang37Jiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaSchool of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaSchool of Stomatology and Medicine, Foshan University, Foshan, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaNanchang Police Dog Base of the Ministry of Public Security of China, Nanchang, ChinaNanchang Police Dog Base of the Ministry of Public Security of China, Nanchang, ChinaNanchang Police Dog Base of the Ministry of Public Security of China, Nanchang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaJiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, ChinaJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, ChinaJiujiang Innovation Center of Biosensor Technology and Application, Jiujiang University, Jiujiang, ChinaCherry eye, the common name for the prolapse of the third eyelid gland in dogs, is a widespread ophthalmic disease affecting dogs of various breeds. This condition severely affects the quality of life of affected dogs, and its underlying cause remains unresolved. In this study, 170K SNP microarray data were collected from 653 brachycephalic dogs and 788 brachycephalic and mesocephalic dogs. These two datasets were analyzed separately in genome-wide association studies (GWAS) involving 12 dog breeds affected by cherry eye. The GWAS analysis of 653 short-headed dogs revealed that four SNPs in the CFA3:15627075-15983629 bp region exceeded the genome-level significance threshold. Association analysis of this region also indicated that these four SNPs were strongly associated. Gene annotation showed that the region contained genes such as KIAA0825, FAM172A, and NR2F1, of which NR2F1 was associated with eye development. The results showed that GWAS analysis performed on 788 short- and medium-headed dogs identified five SNPs in the CFA22:15627075-15983629 bp region that exceeded the genome-level significance threshold, and association analysis was performed in this region, which showed that these five SNPs were strongly associated. In addition, 104 annotated genes were identified in both GWAS. To explore the genes involved in cherry eyes, we performed GO functional enrichment analysis. The genes involved in the high pathway were DIO3 and TTC8. In addition, an in-depth analysis revealed 33 genes associated with eye development and diseases. Our study provides new perspectives for further understanding cherry eye in dogs.https://www.frontiersin.org/articles/10.3389/fvets.2024.1520155/fullcherry eyethird eyelid glandanimal modelcanineeye diseasesgenetics |
| spellingShingle | Yu Zeng Yu Zeng Yu Zeng Cundong Feng Zheli Jiang Zheli Jiang Zheli Jiang Weian Du Shan He Shan He Shan He Xingnuan Li Xingnuan Li Xingnuan Li Yi Fan Yi Fan Yi Fan Xiao Ouyang Xiao Ouyang Xiao Ouyang Bixin Huang Bixin Huang Bixin Huang Yan Su Yan Su Yan Su Siyu Wang Siyu Wang Siyu Wang Rongxing Wei Zonghao Dai Peng Jin Jianyun Liu Jianyun Liu Jianyun Liu Qianyong Yang Qianyong Yang Qianyong Yang Genome-wide association studies with prolapsed gland of the third eyelid in dogs Frontiers in Veterinary Science cherry eye third eyelid gland animal model canine eye diseases genetics |
| title | Genome-wide association studies with prolapsed gland of the third eyelid in dogs |
| title_full | Genome-wide association studies with prolapsed gland of the third eyelid in dogs |
| title_fullStr | Genome-wide association studies with prolapsed gland of the third eyelid in dogs |
| title_full_unstemmed | Genome-wide association studies with prolapsed gland of the third eyelid in dogs |
| title_short | Genome-wide association studies with prolapsed gland of the third eyelid in dogs |
| title_sort | genome wide association studies with prolapsed gland of the third eyelid in dogs |
| topic | cherry eye third eyelid gland animal model canine eye diseases genetics |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1520155/full |
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