Strategies to Identify Recognition Signals and Targets of SUMOylation
SUMOylation contributes to the regulation of many essential cellular factors. Diverse techniques have been used to explore the functional consequences of protein SUMOylation. Most approaches consider the identification of sequences on substrates, adaptors, or receptors regulating the SUMO conjugatio...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2012/875148 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552009974153216 |
|---|---|
| author | Elisa Da Silva-Ferrada Fernando Lopitz-Otsoa Valérie Lang Manuel S. Rodríguez Rune Matthiesen |
| author_facet | Elisa Da Silva-Ferrada Fernando Lopitz-Otsoa Valérie Lang Manuel S. Rodríguez Rune Matthiesen |
| author_sort | Elisa Da Silva-Ferrada |
| collection | DOAJ |
| description | SUMOylation contributes to the regulation of many essential cellular factors. Diverse techniques have been used to explore the functional consequences of protein SUMOylation. Most approaches consider the identification of sequences on substrates, adaptors, or receptors regulating the SUMO conjugation, recognition, or deconjugation. The large majority of the studied SUMOylated proteins contain the sequence [IVL]KxE. SUMOylated proteins are recognized by at least 3 types of hydrophobic SUMO-interacting motifs (SIMs) that contribute to coordinate SUMO-dependent functions. Typically, SIMs are constituted by a hydrophobic core flanked by one or two clusters of negatively charged amino acid residues. Multiple SIMs can integrate SUMO binding domains (SBDs), optimizing binding, and control over SUMO-dependent processes. Here, we present a survey of the methodologies used to study SUMO-regulated functions and provide guidelines for the identification of cis and trans sequences controlling SUMOylation. Furthermore, an integrative analysis of known and putative SUMO substrates illustrates an updated landscape of several SUMO-regulated events. The strategies and analysis presented here should contribute to the understanding of SUMO-controlled functions and provide rational approach to identify biomarkers or choose possible targets for intervention in processes where SUMOylation plays a critical role. |
| format | Article |
| id | doaj-art-4c262d4561ce42e187837938cb3c36c1 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-4c262d4561ce42e187837938cb3c36c12025-02-03T05:59:48ZengWileyBiochemistry Research International2090-22472090-22552012-01-01201210.1155/2012/875148875148Strategies to Identify Recognition Signals and Targets of SUMOylationElisa Da Silva-Ferrada0Fernando Lopitz-Otsoa1Valérie Lang2Manuel S. Rodríguez3Rune Matthiesen4Proteomics Unit, CIC bioGUNE, CIBERehd, Building 801A, Bizkaia Technology Park, 48160 Derio Bizkaia, SpainProteomics Unit, CIC bioGUNE, CIBERehd, Building 801A, Bizkaia Technology Park, 48160 Derio Bizkaia, SpainUbiqutylation and Cancer Molecular Biology Laboratory, Inbiomed, Paseo Mikeletegi 61, 20009 San Sebastian, Gipuzkoa, SpainProteomics Unit, CIC bioGUNE, CIBERehd, Building 801A, Bizkaia Technology Park, 48160 Derio Bizkaia, SpainProteolysis in Diseases Laboratory, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, PortugalSUMOylation contributes to the regulation of many essential cellular factors. Diverse techniques have been used to explore the functional consequences of protein SUMOylation. Most approaches consider the identification of sequences on substrates, adaptors, or receptors regulating the SUMO conjugation, recognition, or deconjugation. The large majority of the studied SUMOylated proteins contain the sequence [IVL]KxE. SUMOylated proteins are recognized by at least 3 types of hydrophobic SUMO-interacting motifs (SIMs) that contribute to coordinate SUMO-dependent functions. Typically, SIMs are constituted by a hydrophobic core flanked by one or two clusters of negatively charged amino acid residues. Multiple SIMs can integrate SUMO binding domains (SBDs), optimizing binding, and control over SUMO-dependent processes. Here, we present a survey of the methodologies used to study SUMO-regulated functions and provide guidelines for the identification of cis and trans sequences controlling SUMOylation. Furthermore, an integrative analysis of known and putative SUMO substrates illustrates an updated landscape of several SUMO-regulated events. The strategies and analysis presented here should contribute to the understanding of SUMO-controlled functions and provide rational approach to identify biomarkers or choose possible targets for intervention in processes where SUMOylation plays a critical role.http://dx.doi.org/10.1155/2012/875148 |
| spellingShingle | Elisa Da Silva-Ferrada Fernando Lopitz-Otsoa Valérie Lang Manuel S. Rodríguez Rune Matthiesen Strategies to Identify Recognition Signals and Targets of SUMOylation Biochemistry Research International |
| title | Strategies to Identify Recognition Signals and Targets of SUMOylation |
| title_full | Strategies to Identify Recognition Signals and Targets of SUMOylation |
| title_fullStr | Strategies to Identify Recognition Signals and Targets of SUMOylation |
| title_full_unstemmed | Strategies to Identify Recognition Signals and Targets of SUMOylation |
| title_short | Strategies to Identify Recognition Signals and Targets of SUMOylation |
| title_sort | strategies to identify recognition signals and targets of sumoylation |
| url | http://dx.doi.org/10.1155/2012/875148 |
| work_keys_str_mv | AT elisadasilvaferrada strategiestoidentifyrecognitionsignalsandtargetsofsumoylation AT fernandolopitzotsoa strategiestoidentifyrecognitionsignalsandtargetsofsumoylation AT valerielang strategiestoidentifyrecognitionsignalsandtargetsofsumoylation AT manuelsrodriguez strategiestoidentifyrecognitionsignalsandtargetsofsumoylation AT runematthiesen strategiestoidentifyrecognitionsignalsandtargetsofsumoylation |