Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway
Cannabinoid compounds have potential as treatments for a variety of conditions, with cannabigerol (CBG) being known for its anti-inflammatory properties. In this study, we investigated the effects of CBG in a cellular model of 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD). In the...
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2025-01-01
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| author | Ga Hee Jeong Ki Chan Kim Ji Hyun Lee |
| author_facet | Ga Hee Jeong Ki Chan Kim Ji Hyun Lee |
| author_sort | Ga Hee Jeong |
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| description | Cannabinoid compounds have potential as treatments for a variety of conditions, with cannabigerol (CBG) being known for its anti-inflammatory properties. In this study, we investigated the effects of CBG in a cellular model of 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD). In the cellular model, we confirmed the cytotoxicity of CBG and downregulated the expression of inflammatory markers <i>CCL26</i>, <i>IL1B</i>, <i>IL6</i>, and <i>TNF</i> (<i>p</i> < 0.001). In the mouse model, clinical, histological, and immunological changes were analyzed. The results showed that CBG improved dermatitis severity score, epidermal thickness, and mast cell count and reduced inflammatory cytokines (<i>Tslp</i>, <i>Il1b</i>, <i>Il4</i>, <i>Il6</i>, <i>Il13</i>, <i>Il17</i>, <i>Il18</i>, <i>Il22</i>, and <i>Il33</i>) by qRT-PCR (<i>p</i> < 0.001). Western blot results showed modulated changes in JAK1, JAK2, TYK2, STAT1, STAT2, STAT3, p-STAT3, STAT6, and p-STAT6 (<i>p</i> < 0.05). Subsequently, p-IκBα, NF-κB, and p-NF-κB signaling factors were also reduced (<i>p</i> < 0.05), with corresponding changes in skin barrier factors. The results of this study indicate that CBG effectively alleviates AD-like symptoms and suggest the potential of CBG as a therapeutic agent. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-4c094e3e300b42a58cb4714d85c336212025-01-24T13:26:36ZengMDPI AGCells2073-44092025-01-011428310.3390/cells14020083Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling PathwayGa Hee Jeong0Ki Chan Kim1Ji Hyun Lee2Department of Medical Sciences, Graduate School of The Catholic University of Korea, Seoul, #222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of KoreaDepartment of Medical Sciences, Graduate School of The Catholic University of Korea, Seoul, #222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of KoreaDepartment of Medical Sciences, Graduate School of The Catholic University of Korea, Seoul, #222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of KoreaCannabinoid compounds have potential as treatments for a variety of conditions, with cannabigerol (CBG) being known for its anti-inflammatory properties. In this study, we investigated the effects of CBG in a cellular model of 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD). In the cellular model, we confirmed the cytotoxicity of CBG and downregulated the expression of inflammatory markers <i>CCL26</i>, <i>IL1B</i>, <i>IL6</i>, and <i>TNF</i> (<i>p</i> < 0.001). In the mouse model, clinical, histological, and immunological changes were analyzed. The results showed that CBG improved dermatitis severity score, epidermal thickness, and mast cell count and reduced inflammatory cytokines (<i>Tslp</i>, <i>Il1b</i>, <i>Il4</i>, <i>Il6</i>, <i>Il13</i>, <i>Il17</i>, <i>Il18</i>, <i>Il22</i>, and <i>Il33</i>) by qRT-PCR (<i>p</i> < 0.001). Western blot results showed modulated changes in JAK1, JAK2, TYK2, STAT1, STAT2, STAT3, p-STAT3, STAT6, and p-STAT6 (<i>p</i> < 0.05). Subsequently, p-IκBα, NF-κB, and p-NF-κB signaling factors were also reduced (<i>p</i> < 0.05), with corresponding changes in skin barrier factors. The results of this study indicate that CBG effectively alleviates AD-like symptoms and suggest the potential of CBG as a therapeutic agent.https://www.mdpi.com/2073-4409/14/2/83cannabigerolJanus kinase-signal transducer and activator of transcriptionatopic dermatitis |
| spellingShingle | Ga Hee Jeong Ki Chan Kim Ji Hyun Lee Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway Cells cannabigerol Janus kinase-signal transducer and activator of transcription atopic dermatitis |
| title | Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway |
| title_full | Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway |
| title_fullStr | Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway |
| title_full_unstemmed | Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway |
| title_short | Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway |
| title_sort | anti inflammatory effects of cannabigerol in vitro and in vivo are mediated through the jak stat nfκb signaling pathway |
| topic | cannabigerol Janus kinase-signal transducer and activator of transcription atopic dermatitis |
| url | https://www.mdpi.com/2073-4409/14/2/83 |
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