An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro
Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term wi...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/2157451 |
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| author | Li Zhang Yenan Wu Xiang Li Shao Wei Yiming Xing Zhengxing Lian Hongbing Han |
| author_facet | Li Zhang Yenan Wu Xiang Li Shao Wei Yiming Xing Zhengxing Lian Hongbing Han |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of DF-1 feeder and basic culture medium supplemented with human basic fibroblast growth factor (hbFGF), mouse stem cell factor (mSCF), and human leukemia inhibitory factor (hLIF). During the long culture period, the cESCs showed typical ES cell morphology and expressed primitive stem cell markers with a relatively stable proliferation rate and high telomerase activity. These cells also exhibited the capability to differentiate into cardiac myocytes, smooth muscle cells, neural cells, osteoblast, and adipocyte in vitro. Chimera chickens were produced by cESCs cultured for 25 passages with this new culture system. The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro. |
| format | Article |
| id | doaj-art-4c0390ddd8704e4cac5148e0d2923994 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4c0390ddd8704e4cac5148e0d29239942025-02-03T05:50:48ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/21574512157451An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In VitroLi Zhang0Yenan Wu1Xiang Li2Shao Wei3Yiming Xing4Zhengxing Lian5Hongbing Han6Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaChicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of DF-1 feeder and basic culture medium supplemented with human basic fibroblast growth factor (hbFGF), mouse stem cell factor (mSCF), and human leukemia inhibitory factor (hLIF). During the long culture period, the cESCs showed typical ES cell morphology and expressed primitive stem cell markers with a relatively stable proliferation rate and high telomerase activity. These cells also exhibited the capability to differentiate into cardiac myocytes, smooth muscle cells, neural cells, osteoblast, and adipocyte in vitro. Chimera chickens were produced by cESCs cultured for 25 passages with this new culture system. The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro.http://dx.doi.org/10.1155/2018/2157451 |
| spellingShingle | Li Zhang Yenan Wu Xiang Li Shao Wei Yiming Xing Zhengxing Lian Hongbing Han An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro Stem Cells International |
| title | An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro |
| title_full | An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro |
| title_fullStr | An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro |
| title_full_unstemmed | An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro |
| title_short | An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro |
| title_sort | alternative method for long term culture of chicken embryonic stem cell in vitro |
| url | http://dx.doi.org/10.1155/2018/2157451 |
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