PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
Amylases are used in numerous industrial applications for converting starch into products of greater value. This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize t...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Viçosa (UFV)
2020-09-01
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| Series: | The Journal of Engineering and Exact Sciences |
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| Online Access: | https://periodicos.ufv.br/jcec/article/view/10560 |
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| author | Mariana Tainná Silva Souza Barbhara Mota Marinho Thiago Machado Pasin David Lee Nelson Vivian Machado Benassi |
| author_facet | Mariana Tainná Silva Souza Barbhara Mota Marinho Thiago Machado Pasin David Lee Nelson Vivian Machado Benassi |
| author_sort | Mariana Tainná Silva Souza |
| collection | DOAJ |
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Amylases are used in numerous industrial applications for converting starch into products of greater value. This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize the parameters for the cultivation of the microorganism and biochemically characterize the amylase. Among 21 filamentous fungi isolated in Janaúba, state of Minas Gerais, Brazil, the best amylase producer was selected for standardization of culture parameters and subsequent enzymatic characterization. Maximum activity was obtained in CP medium after six days of cultivation at 30 °C. Amylases produced by this fungus are stable to variations in pH and temperature, exhibited optimum activities at 65 oC and pH 6.0, and were significantly activated in the presence of 5 and 10 mm KH2PO4.
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| format | Article |
| id | doaj-art-4bee4189a35044539127dfe8a23f8504 |
| institution | Kabale University |
| issn | 2527-1075 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Universidade Federal de Viçosa (UFV) |
| record_format | Article |
| series | The Journal of Engineering and Exact Sciences |
| spelling | doaj-art-4bee4189a35044539127dfe8a23f85042025-02-02T19:58:16ZengUniversidade Federal de Viçosa (UFV)The Journal of Engineering and Exact Sciences2527-10752020-09-016310.18540/jcecvl6iss3pp0365-0376PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2Mariana Tainná Silva SouzaBarbhara Mota MarinhoThiago Machado PasinDavid Lee NelsonVivian Machado Benassi Amylases are used in numerous industrial applications for converting starch into products of greater value. This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize the parameters for the cultivation of the microorganism and biochemically characterize the amylase. Among 21 filamentous fungi isolated in Janaúba, state of Minas Gerais, Brazil, the best amylase producer was selected for standardization of culture parameters and subsequent enzymatic characterization. Maximum activity was obtained in CP medium after six days of cultivation at 30 °C. Amylases produced by this fungus are stable to variations in pH and temperature, exhibited optimum activities at 65 oC and pH 6.0, and were significantly activated in the presence of 5 and 10 mm KH2PO4. https://periodicos.ufv.br/jcec/article/view/10560EnzimasFungos FilamentososBioprospecçãoBiotecnologia |
| spellingShingle | Mariana Tainná Silva Souza Barbhara Mota Marinho Thiago Machado Pasin David Lee Nelson Vivian Machado Benassi PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2 The Journal of Engineering and Exact Sciences Enzimas Fungos Filamentosos Bioprospecção Biotecnologia |
| title | PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2 |
| title_full | PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2 |
| title_fullStr | PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2 |
| title_full_unstemmed | PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2 |
| title_short | PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2 |
| title_sort | prospection of filamentous fungi and the production of amylase by aspergillus sp m1 7 2 |
| topic | Enzimas Fungos Filamentosos Bioprospecção Biotecnologia |
| url | https://periodicos.ufv.br/jcec/article/view/10560 |
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