Properties of Actinobacillus pleuropneumoniae isolates
Results of tests of six Actinobacillus pleuropneumoniae isolates recovered from the diseased pigs kept in animal holdings located in the Russian Federation for their biological properties (biochemical, proteomic, antigenic and pathogenic ones) are presented in the paper. Proteomic properties were de...
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Da Vinci Media
2023-06-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/734 |
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| author | V. А. Evgrafova О. V. Pruntova N. B. Shadrova А. М. Timina |
| author_facet | V. А. Evgrafova О. V. Pruntova N. B. Shadrova А. М. Timina |
| author_sort | V. А. Evgrafova |
| collection | DOAJ |
| description | Results of tests of six Actinobacillus pleuropneumoniae isolates recovered from the diseased pigs kept in animal holdings located in the Russian Federation for their biological properties (biochemical, proteomic, antigenic and pathogenic ones) are presented in the paper. Proteomic properties were determined with mass-spectrometry using Autof MS 1000 mass-spectrometer (Autobio Diagnostics Co., Ltd, China): protein profiles were plotted and the peaks characteristic for each isolate were identified. Mass-spectra of tested Actinobacillus isolates and reference Actinobacillus pleuropneumoniae DSM 13472 strain were found to be in the m/z range of 2,000–12,000 Da. The following peaks (m/z) were common for all Actinobacillus pleuropneumoniae isolates and the strain: 2,541 ± 2; 4,267 ± 2; 5,085 ± 2; 6,450 ± 2; 7,207 ± 4; 9,408 ± 3; 11,820 ± 6. Therewith, the highest intensity (100%) was reported for the peak at 5,085 ± 2, that was supposed to be a specific feature of Actinobacillus pleuropneumoniae. All isolates were confirmed to belong to Actinobacillus pleuropneumoniae species and to 2, 5 and 9 serotypes by real-time polymerase chain reaction using species-specific and serotype-specific primers. Actinobacillus рleuropneumoniae isolates were tested for their pathogenic properties by experimental infection of white mice and 2.5–3 month-old piglets. All tested isolates were pathogenic for both white mice and piglets. Isolate No. 4 belonging to serotype 5 was found to be the most virulent out of tested isolates. Thus, LD50 was 4.19 lg microbial cells for white mice and 5.49 lg microbial cells for piglets that was consistent to the data of other authors carried out tests of actinobaccilli isolated in the Russian Federation for their pathogenicity. The isolates were deposited to the FGBI “ARRIAH” Collection of Microorganism Strains. |
| format | Article |
| id | doaj-art-4bbdcc131af4434ca4743196de3c0073 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-4bbdcc131af4434ca4743196de3c00732025-02-06T09:52:10ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592023-06-0112217818410.29326/2304-196X-2023-12-2-178-184620Properties of Actinobacillus pleuropneumoniae isolatesV. А. Evgrafova0О. V. Pruntova1N. B. Shadrova2А. М. Timina3FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)Results of tests of six Actinobacillus pleuropneumoniae isolates recovered from the diseased pigs kept in animal holdings located in the Russian Federation for their biological properties (biochemical, proteomic, antigenic and pathogenic ones) are presented in the paper. Proteomic properties were determined with mass-spectrometry using Autof MS 1000 mass-spectrometer (Autobio Diagnostics Co., Ltd, China): protein profiles were plotted and the peaks characteristic for each isolate were identified. Mass-spectra of tested Actinobacillus isolates and reference Actinobacillus pleuropneumoniae DSM 13472 strain were found to be in the m/z range of 2,000–12,000 Da. The following peaks (m/z) were common for all Actinobacillus pleuropneumoniae isolates and the strain: 2,541 ± 2; 4,267 ± 2; 5,085 ± 2; 6,450 ± 2; 7,207 ± 4; 9,408 ± 3; 11,820 ± 6. Therewith, the highest intensity (100%) was reported for the peak at 5,085 ± 2, that was supposed to be a specific feature of Actinobacillus pleuropneumoniae. All isolates were confirmed to belong to Actinobacillus pleuropneumoniae species and to 2, 5 and 9 serotypes by real-time polymerase chain reaction using species-specific and serotype-specific primers. Actinobacillus рleuropneumoniae isolates were tested for their pathogenic properties by experimental infection of white mice and 2.5–3 month-old piglets. All tested isolates were pathogenic for both white mice and piglets. Isolate No. 4 belonging to serotype 5 was found to be the most virulent out of tested isolates. Thus, LD50 was 4.19 lg microbial cells for white mice and 5.49 lg microbial cells for piglets that was consistent to the data of other authors carried out tests of actinobaccilli isolated in the Russian Federation for their pathogenicity. The isolates were deposited to the FGBI “ARRIAH” Collection of Microorganism Strains.https://veterinary.arriah.ru/jour/article/view/734porcine (actinobacillus) pleuropneumoniaisolatepropertiespolymerase chain reactionmass-spectrometrypathogenicity |
| spellingShingle | V. А. Evgrafova О. V. Pruntova N. B. Shadrova А. М. Timina Properties of Actinobacillus pleuropneumoniae isolates Ветеринария сегодня porcine (actinobacillus) pleuropneumonia isolate properties polymerase chain reaction mass-spectrometry pathogenicity |
| title | Properties of Actinobacillus pleuropneumoniae isolates |
| title_full | Properties of Actinobacillus pleuropneumoniae isolates |
| title_fullStr | Properties of Actinobacillus pleuropneumoniae isolates |
| title_full_unstemmed | Properties of Actinobacillus pleuropneumoniae isolates |
| title_short | Properties of Actinobacillus pleuropneumoniae isolates |
| title_sort | properties of actinobacillus pleuropneumoniae isolates |
| topic | porcine (actinobacillus) pleuropneumonia isolate properties polymerase chain reaction mass-spectrometry pathogenicity |
| url | https://veterinary.arriah.ru/jour/article/view/734 |
| work_keys_str_mv | AT vaevgrafova propertiesofactinobacilluspleuropneumoniaeisolates AT ovpruntova propertiesofactinobacilluspleuropneumoniaeisolates AT nbshadrova propertiesofactinobacilluspleuropneumoniaeisolates AT amtimina propertiesofactinobacilluspleuropneumoniaeisolates |