A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae)
We analysed the embryonic epidermis of the pool frog, Pelophylax lessonae, by light and electron microscopy techniques, and characterized preliminarily its mucous secretions with conventional histochemistry (Toluidine Blue, Periodic Acid-Schiff, Alcian blue pH 2.5 and Alcian blue pH 1.0). At Gosner’...
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Taylor & Francis Group
2024-07-01
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| Series: | The European Zoological Journal |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/24750263.2024.2422089 |
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| author | D. SEMERARO D. MENTINO R. ROSSI G. SCILLITANI M. V. GUGLIELMI M. MASTRODONATO |
| author_facet | D. SEMERARO D. MENTINO R. ROSSI G. SCILLITANI M. V. GUGLIELMI M. MASTRODONATO |
| author_sort | D. SEMERARO |
| collection | DOAJ |
| description | We analysed the embryonic epidermis of the pool frog, Pelophylax lessonae, by light and electron microscopy techniques, and characterized preliminarily its mucous secretions with conventional histochemistry (Toluidine Blue, Periodic Acid-Schiff, Alcian blue pH 2.5 and Alcian blue pH 1.0). At Gosner’s developmental stage 21, the epidermis consisted of a bilayered epithelium with five cell types. Basal cells (BCs) constituted the basal layer, presented a large nucleus and were sometimes observed in mitosis. Ciliated cells (CCs) had a ciliated apical membrane, with carboxylated glycans in the sub-apical portion. TEM analysis revealed small vesicles just below the surface, a central nucleus, and evident yolk plates. Goblet cells (GCs) and small secretory cells (SSCs) showed secreting vesicles and vacuoles, respectively. In the mucus secretion of GCs sialosulphomucins appeared, while in that of SSCs sulphation was not observed. Under TEM, GCs had irregular shaped nuclei and a less electron-dense cytoplasm with respect to SSCs. Ionocytes (INs) presented apical microvilli and a high electron-dense cytoplasm with vacuoles. SEM analysis revealed that GCs outnumbered the other cell types and that SSCs and INs were usually located together near to GCs. The relatively simple structure of the epidermis and its direct interfacing with the environment renders it a good model for the study of structure and development of mucociliary epithelia, as well as of its alterations in the presence of toxicants. |
| format | Article |
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| institution | OA Journals |
| issn | 2475-0263 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | The European Zoological Journal |
| spelling | doaj-art-4bb1a0a3bfcf4e0299db16e0b5ce2e9d2025-08-20T02:19:22ZengTaylor & Francis GroupThe European Zoological Journal2475-02632024-07-019121167117710.1080/24750263.2024.2422089A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae)D. SEMERARO0D. MENTINO1R. ROSSI2G. SCILLITANI3M. V. GUGLIELMI4M. MASTRODONATO5Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, Bari, ItalyDepartment of Precision and Regenerative Medicine and Ionian Area, University of Bari Aldo Moro, Bari, ItalyDepartment of Precision and Regenerative Medicine and Ionian Area, University of Bari Aldo Moro, Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, Bari, ItalyInstitute for Water Research (IRSA), National Research Council (CNR), Taranto, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, Bari, ItalyWe analysed the embryonic epidermis of the pool frog, Pelophylax lessonae, by light and electron microscopy techniques, and characterized preliminarily its mucous secretions with conventional histochemistry (Toluidine Blue, Periodic Acid-Schiff, Alcian blue pH 2.5 and Alcian blue pH 1.0). At Gosner’s developmental stage 21, the epidermis consisted of a bilayered epithelium with five cell types. Basal cells (BCs) constituted the basal layer, presented a large nucleus and were sometimes observed in mitosis. Ciliated cells (CCs) had a ciliated apical membrane, with carboxylated glycans in the sub-apical portion. TEM analysis revealed small vesicles just below the surface, a central nucleus, and evident yolk plates. Goblet cells (GCs) and small secretory cells (SSCs) showed secreting vesicles and vacuoles, respectively. In the mucus secretion of GCs sialosulphomucins appeared, while in that of SSCs sulphation was not observed. Under TEM, GCs had irregular shaped nuclei and a less electron-dense cytoplasm with respect to SSCs. Ionocytes (INs) presented apical microvilli and a high electron-dense cytoplasm with vacuoles. SEM analysis revealed that GCs outnumbered the other cell types and that SSCs and INs were usually located together near to GCs. The relatively simple structure of the epidermis and its direct interfacing with the environment renders it a good model for the study of structure and development of mucociliary epithelia, as well as of its alterations in the presence of toxicants.https://www.tandfonline.com/doi/10.1080/24750263.2024.2422089Pelophylax lessonaeembryologymucociliary epidermishistochemistryultrastructure |
| spellingShingle | D. SEMERARO D. MENTINO R. ROSSI G. SCILLITANI M. V. GUGLIELMI M. MASTRODONATO A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae) The European Zoological Journal Pelophylax lessonae embryology mucociliary epidermis histochemistry ultrastructure |
| title | A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae) |
| title_full | A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae) |
| title_fullStr | A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae) |
| title_full_unstemmed | A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae) |
| title_short | A microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog, Pelophylax lessonae (Camerano, 1882) (Anura: Ranidae) |
| title_sort | microscopical and ultrastructural analysis of the embryonic epidermis of the pool frog pelophylax lessonae camerano 1882 anura ranidae |
| topic | Pelophylax lessonae embryology mucociliary epidermis histochemistry ultrastructure |
| url | https://www.tandfonline.com/doi/10.1080/24750263.2024.2422089 |
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