Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand
Sand flies are principal vectors of Leishmania spp. and Trypanosoma spp. Identifying precise vector species is crucial for effective control. We conducted a study on the species distribution of phlebotomine sand flies in cave-dwelling and non-cave-dwelling in four southern provinces of Thailand. In...
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Elsevier
2025-01-01
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| Series: | Current Research in Parasitology and Vector-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667114X25000238 |
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| author | Nantatchaporn Klaiklueng Rawadee Kumlert Sopavadee Moonmake Toon Ruang-areerate Padet Siriyasatien Sakone Sunantaraporn Darawan Wanachiwanawin Pichet Ruenchit Sirichit Wongkamchai |
| author_facet | Nantatchaporn Klaiklueng Rawadee Kumlert Sopavadee Moonmake Toon Ruang-areerate Padet Siriyasatien Sakone Sunantaraporn Darawan Wanachiwanawin Pichet Ruenchit Sirichit Wongkamchai |
| author_sort | Nantatchaporn Klaiklueng |
| collection | DOAJ |
| description | Sand flies are principal vectors of Leishmania spp. and Trypanosoma spp. Identifying precise vector species is crucial for effective control. We conducted a study on the species distribution of phlebotomine sand flies in cave-dwelling and non-cave-dwelling in four southern provinces of Thailand. In this study, we collected 621 sand flies (346 females and 275 males) and identified all specimens based on morphology and DNA barcoding, employing cytochrome c oxidase subunit 1 (cox1) and cytochrome b (cytb) genes. In female specimens, we also screened the small subunit 18S ribosomal RNA (18S rRNA) gene for Leishmania spp. and Trypanosoma spp. Morphologically, 467 (75.2%) sand flies were identified to species level, 47 (7.57%) to subgenus level, and 107 (17.23%) to genus level. These included Idiophlebotomus asperulus (43.48%), Sergentomyia khawi (26.73%), S. anodontis (2.25%), S. brevicaulis (2.25%), Grassomyia indica (0.48%), Phlebotomus (Euphlebotomus) spp. (4.83%), Phlebotomus (Lewisius) spp. (2.74%), Sergentomyia spp. (9.18%), and Phlebotomus spp. (8.05%). Among the 107 specimens identified to genus level, DNA barcoding further identified 49 (45.79%) as Sergentomyia barraudi (1.61%), S. bailyi (0.16%), Phlebotomus kiangsuensis (2.9%), and Ph. stantoni (1.61%). No Leishmania DNA was detected, but Trypanosoma DNA was found in females of S. khawi from Narathiwat Province. Expanding genetic reference databases of sand flies located in four provinces of southern Thailand will improve barcoding accuracy. Understanding sand fly species composition and distribution is imperative for vector control and disease prevention in Thailand. |
| format | Article |
| id | doaj-art-4baf96a3d3b54a4f9b7b645c2f430a4d |
| institution | Kabale University |
| issn | 2667-114X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Parasitology and Vector-Borne Diseases |
| spelling | doaj-art-4baf96a3d3b54a4f9b7b645c2f430a4d2025-08-20T03:31:06ZengElsevierCurrent Research in Parasitology and Vector-Borne Diseases2667-114X2025-01-01710026310.1016/j.crpvbd.2025.100263Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of ThailandNantatchaporn Klaiklueng0Rawadee Kumlert1Sopavadee Moonmake2Toon Ruang-areerate3Padet Siriyasatien4Sakone Sunantaraporn5Darawan Wanachiwanawin6Pichet Ruenchit7Sirichit Wongkamchai8Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, ThailandDivision of Vector Borne Diseases, Department of Disease Control, Ministry of Public Health, Nonthaburi, 11000, ThailandThe Office of Disease Prevention Control 12, Songkla, 90000, ThailandDepartment of Parasitology, Phramongkutklao College of Medicine, Bangkok, 10400, ThailandCenter of Excellence in Vector Biology and Vector Borne Diseases, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandCenter of Excellence in Vector Biology and Vector Borne Diseases, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, ThailandDepartment of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, ThailandDepartment of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; Corresponding author. Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkok Noi, Bangkok, 10700, Thailand.Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; Corresponding author. Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkok Noi, Bangkok, 10700, Thailand.Sand flies are principal vectors of Leishmania spp. and Trypanosoma spp. Identifying precise vector species is crucial for effective control. We conducted a study on the species distribution of phlebotomine sand flies in cave-dwelling and non-cave-dwelling in four southern provinces of Thailand. In this study, we collected 621 sand flies (346 females and 275 males) and identified all specimens based on morphology and DNA barcoding, employing cytochrome c oxidase subunit 1 (cox1) and cytochrome b (cytb) genes. In female specimens, we also screened the small subunit 18S ribosomal RNA (18S rRNA) gene for Leishmania spp. and Trypanosoma spp. Morphologically, 467 (75.2%) sand flies were identified to species level, 47 (7.57%) to subgenus level, and 107 (17.23%) to genus level. These included Idiophlebotomus asperulus (43.48%), Sergentomyia khawi (26.73%), S. anodontis (2.25%), S. brevicaulis (2.25%), Grassomyia indica (0.48%), Phlebotomus (Euphlebotomus) spp. (4.83%), Phlebotomus (Lewisius) spp. (2.74%), Sergentomyia spp. (9.18%), and Phlebotomus spp. (8.05%). Among the 107 specimens identified to genus level, DNA barcoding further identified 49 (45.79%) as Sergentomyia barraudi (1.61%), S. bailyi (0.16%), Phlebotomus kiangsuensis (2.9%), and Ph. stantoni (1.61%). No Leishmania DNA was detected, but Trypanosoma DNA was found in females of S. khawi from Narathiwat Province. Expanding genetic reference databases of sand flies located in four provinces of southern Thailand will improve barcoding accuracy. Understanding sand fly species composition and distribution is imperative for vector control and disease prevention in Thailand.http://www.sciencedirect.com/science/article/pii/S2667114X25000238cox1cytb18S rRNA geneDNA barcodingLeishmaniaTrypanosoma |
| spellingShingle | Nantatchaporn Klaiklueng Rawadee Kumlert Sopavadee Moonmake Toon Ruang-areerate Padet Siriyasatien Sakone Sunantaraporn Darawan Wanachiwanawin Pichet Ruenchit Sirichit Wongkamchai Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand Current Research in Parasitology and Vector-Borne Diseases cox1 cytb 18S rRNA gene DNA barcoding Leishmania Trypanosoma |
| title | Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand |
| title_full | Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand |
| title_fullStr | Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand |
| title_full_unstemmed | Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand |
| title_short | Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand |
| title_sort | species distribution and screening of trypanosoma dna in phlebotomine sand flies from four southern provinces of thailand |
| topic | cox1 cytb 18S rRNA gene DNA barcoding Leishmania Trypanosoma |
| url | http://www.sciencedirect.com/science/article/pii/S2667114X25000238 |
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