Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma
<b>Background</b>: Severe and difficult-to-control asthma occurs in 3–10% of patients in developed countries. The aim of our study was to investigate the association of the prognostic role of leptin and adiponectin, as well as the leptin receptor gene polymorphism Gln223Arg, in patients...
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2025-05-01
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| author | Saule Maimysheva Lyudmila Karazhanova Andrey Orekhov Assel Chinybayeva Bolat Ashirov |
| author_facet | Saule Maimysheva Lyudmila Karazhanova Andrey Orekhov Assel Chinybayeva Bolat Ashirov |
| author_sort | Saule Maimysheva |
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| description | <b>Background</b>: Severe and difficult-to-control asthma occurs in 3–10% of patients in developed countries. The aim of our study was to investigate the association of the prognostic role of leptin and adiponectin, as well as the leptin receptor gene polymorphism Gln223Arg, in patients with difficult-to-control and severe asthma. <b>Methods</b>: The present study included 200 patients with asthma hospitalized in the Department of Pulmonology between January 2018 and December 2021. In all patients, in addition to routine clinical investigations, adiponectin, leptin and their ratio were analyzed, as well as levels of pro-inflammatory cytokines (IL-6, IL-8 and TNF-alpha). External respiratory function was also assessed. LEPR Gln223Arg single-nucleotide polymorphisms were genotyped by real-time PCR method. <b>Results</b>: Patients were randomized into two groups, depending on the severity of asthma: an uncontrolled asthma group and a controlled asthma group, according to the GINA criteria. Among patients with uncontrolled asthma, 101 subjects (74.3%) had metabolic syndrome (<i>p</i> < 0.001). There was an inverse association of the adiponectin/leptin ratio with the eosinophil count (B = −0.305, <i>p</i> < 0.001), IL-6 (B = −0.026, <i>p</i> < 0.001), IL-8 (B = −0.062, <i>p</i> < 0.001) and TNF-alpha (B = −0.047, <i>p</i> < 0.001) and a direct correlation with the level of FEV1 (B = 0.121, <i>p</i> < 0.001) and FVC (B = 0.104, <i>p</i> < 0.001). A probable association of homozygous A/A allele with increased risk of uncontrolled asthma was shown (<i>p</i> = 0.007). <b>Conclusions</b>: Leptin receptor polymorphism with A/A genotype may be associated with a higher probability of developing severe and difficult-to-control asthma. |
| format | Article |
| id | doaj-art-4b9721964e4e46c19ca9dd6bc4214e1e |
| institution | OA Journals |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Diagnostics |
| spelling | doaj-art-4b9721964e4e46c19ca9dd6bc4214e1e2025-08-20T01:49:28ZengMDPI AGDiagnostics2075-44182025-05-01159115410.3390/diagnostics15091154Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe AsthmaSaule Maimysheva0Lyudmila Karazhanova1Andrey Orekhov2Assel Chinybayeva3Bolat Ashirov4Department of Internal Medicine, Semey Medical University, Semey 071400, KazakhstanDepartment of Internal Medicine, Semey Medical University, Semey 071400, KazakhstanDepartment of Internal Medicine, Semey Medical University, Semey 071400, KazakhstanCardiology Department, Astana Medical University, Astana 010000, KazakhstanDepartment of Internal Medicine and Cardiology, South Kazakhstan Medical Academy, Shymkent 160019, Kazakhstan<b>Background</b>: Severe and difficult-to-control asthma occurs in 3–10% of patients in developed countries. The aim of our study was to investigate the association of the prognostic role of leptin and adiponectin, as well as the leptin receptor gene polymorphism Gln223Arg, in patients with difficult-to-control and severe asthma. <b>Methods</b>: The present study included 200 patients with asthma hospitalized in the Department of Pulmonology between January 2018 and December 2021. In all patients, in addition to routine clinical investigations, adiponectin, leptin and their ratio were analyzed, as well as levels of pro-inflammatory cytokines (IL-6, IL-8 and TNF-alpha). External respiratory function was also assessed. LEPR Gln223Arg single-nucleotide polymorphisms were genotyped by real-time PCR method. <b>Results</b>: Patients were randomized into two groups, depending on the severity of asthma: an uncontrolled asthma group and a controlled asthma group, according to the GINA criteria. Among patients with uncontrolled asthma, 101 subjects (74.3%) had metabolic syndrome (<i>p</i> < 0.001). There was an inverse association of the adiponectin/leptin ratio with the eosinophil count (B = −0.305, <i>p</i> < 0.001), IL-6 (B = −0.026, <i>p</i> < 0.001), IL-8 (B = −0.062, <i>p</i> < 0.001) and TNF-alpha (B = −0.047, <i>p</i> < 0.001) and a direct correlation with the level of FEV1 (B = 0.121, <i>p</i> < 0.001) and FVC (B = 0.104, <i>p</i> < 0.001). A probable association of homozygous A/A allele with increased risk of uncontrolled asthma was shown (<i>p</i> = 0.007). <b>Conclusions</b>: Leptin receptor polymorphism with A/A genotype may be associated with a higher probability of developing severe and difficult-to-control asthma.https://www.mdpi.com/2075-4418/15/9/1154asthmametabolic syndromeobesityinterleukineleptin receptorpolymorphism |
| spellingShingle | Saule Maimysheva Lyudmila Karazhanova Andrey Orekhov Assel Chinybayeva Bolat Ashirov Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma Diagnostics asthma metabolic syndrome obesity interleukine leptin receptor polymorphism |
| title | Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma |
| title_full | Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma |
| title_fullStr | Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma |
| title_full_unstemmed | Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma |
| title_short | Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma |
| title_sort | evaluation of adipokine status and leptin receptor gene polymorphism in patients with severe asthma |
| topic | asthma metabolic syndrome obesity interleukine leptin receptor polymorphism |
| url | https://www.mdpi.com/2075-4418/15/9/1154 |
| work_keys_str_mv | AT saulemaimysheva evaluationofadipokinestatusandleptinreceptorgenepolymorphisminpatientswithsevereasthma AT lyudmilakarazhanova evaluationofadipokinestatusandleptinreceptorgenepolymorphisminpatientswithsevereasthma AT andreyorekhov evaluationofadipokinestatusandleptinreceptorgenepolymorphisminpatientswithsevereasthma AT asselchinybayeva evaluationofadipokinestatusandleptinreceptorgenepolymorphisminpatientswithsevereasthma AT bolatashirov evaluationofadipokinestatusandleptinreceptorgenepolymorphisminpatientswithsevereasthma |