The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation
Recognition mechanisms of innate immune response help to improve immunotherapeutic strategies in HBeAg-negative chronic hepatitis B (CHB). Toll-like receptor 2 (TLR2) is an important component of innate immunity. In this study, the frequency of precore mutations of the hepatitis B virus (HBV) and se...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2013/780319 |
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| author | Malihe Moradzadeh Sirous Tayebi Hossein Poustchi Kourosh Sayehmiri Parisa Shahnazari Elnaz Naderi Ghodratollah Montazeri Ashraf Mohamadkhani |
| author_facet | Malihe Moradzadeh Sirous Tayebi Hossein Poustchi Kourosh Sayehmiri Parisa Shahnazari Elnaz Naderi Ghodratollah Montazeri Ashraf Mohamadkhani |
| author_sort | Malihe Moradzadeh |
| collection | DOAJ |
| description | Recognition mechanisms of innate immune response help to improve immunotherapeutic strategies in HBeAg-negative chronic hepatitis B (CHB). Toll-like receptor 2 (TLR2) is an important component of innate immunity. In this study, the frequency of precore mutations of the hepatitis B virus (HBV) and serum TLR2 were evaluated in CHB patients. Fifty-one patients with chronic hepatitis B, negative for HBeAg and detectable HBV DNA, were examined for the presence of mutations in pre-core region of HBV genome by direct sequencing. Serum TLR2 was measured by enzyme-linked immunosorbent assay. Interactions of truncated HBeAg and TLR2 proteins were evaluated with molecular docking software. The G1896A pre-core mutation were detected in 29 (57%) which was significantly associated with higher concentration of serum TLR2 in comparison with patients without this mutation (4.8 ± 2.9 versus 3.4 ± 2.2 ng/mL, P=0.03). There was also a significant correlation between serum ALT and TLR-2 (r=0.46; P=0.01). Docking results illustrated residues within the N-terminus of truncated HBeAg and TLR2, which might facilitate the interaction of these proteins. These findings showed the dominance of G1896A pre-core mutation of HBV variants in this community which was correlated with serum TLR2. Moreover TLR2 is critical for induction of inflammatory cytokines and therefore ALT elevation. |
| format | Article |
| id | doaj-art-4b8a1f842a33435f867c9177bef78493 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-4b8a1f842a33435f867c9177bef784932025-02-03T00:58:54ZengWileyAdvances in Virology1687-86391687-86472013-01-01201310.1155/2013/780319780319The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore MutationMalihe Moradzadeh0Sirous Tayebi1Hossein Poustchi2Kourosh Sayehmiri3Parisa Shahnazari4Elnaz Naderi5Ghodratollah Montazeri6Ashraf Mohamadkhani7Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranLiver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranLiver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranPsychosocial Injuries Research Centre, Ilam University of Medical Sciences, Ilam, IranMonoclonal Antibody Research Centre, Avicenna Research Institute, ACECR, Tehran, IranDepartment of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranLiver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranLiver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranRecognition mechanisms of innate immune response help to improve immunotherapeutic strategies in HBeAg-negative chronic hepatitis B (CHB). Toll-like receptor 2 (TLR2) is an important component of innate immunity. In this study, the frequency of precore mutations of the hepatitis B virus (HBV) and serum TLR2 were evaluated in CHB patients. Fifty-one patients with chronic hepatitis B, negative for HBeAg and detectable HBV DNA, were examined for the presence of mutations in pre-core region of HBV genome by direct sequencing. Serum TLR2 was measured by enzyme-linked immunosorbent assay. Interactions of truncated HBeAg and TLR2 proteins were evaluated with molecular docking software. The G1896A pre-core mutation were detected in 29 (57%) which was significantly associated with higher concentration of serum TLR2 in comparison with patients without this mutation (4.8 ± 2.9 versus 3.4 ± 2.2 ng/mL, P=0.03). There was also a significant correlation between serum ALT and TLR-2 (r=0.46; P=0.01). Docking results illustrated residues within the N-terminus of truncated HBeAg and TLR2, which might facilitate the interaction of these proteins. These findings showed the dominance of G1896A pre-core mutation of HBV variants in this community which was correlated with serum TLR2. Moreover TLR2 is critical for induction of inflammatory cytokines and therefore ALT elevation.http://dx.doi.org/10.1155/2013/780319 |
| spellingShingle | Malihe Moradzadeh Sirous Tayebi Hossein Poustchi Kourosh Sayehmiri Parisa Shahnazari Elnaz Naderi Ghodratollah Montazeri Ashraf Mohamadkhani The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation Advances in Virology |
| title | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation |
| title_full | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation |
| title_fullStr | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation |
| title_full_unstemmed | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation |
| title_short | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation |
| title_sort | possible role of tlr2 in chronic hepatitis b patients with precore mutation |
| url | http://dx.doi.org/10.1155/2013/780319 |
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