Beneficial and Safety Properties of a Bacteriocinogenic and Putative Probiotic <i>Latilactobacillus sakei</i> subsp. <i>sakei</i> 2a Strain

Beneficial and Safety Properties of a Bacteriocinogenic and Putative Probiotic <i>Latilactobacillus sakei</i> subsp. <i>sakei</i> 2a Strain

This work aimed to evaluate some of the probiotic features and safety of the bacteriocin-producing <i>Latilactobacillus sakei</i> subsp. <i>sakei</i> 2a. The effect of selected commercial drugs from different generic groups and antibiotics on the growth of <i>Ltb. sakei...

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Bibliographic Details
Main Authors: Tatiana Alexandrovna Lipilkina, Cristhian Xu, Matheus de Souza Barbosa, Valentina Nikolaevna Khramova, Sergei K. Shebeko, Alexey M. Ermakov, Iskra Vitanova Ivanova, Svetoslav Dimitrov Todorov
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Foods
Subjects:
<i>Latilactobacillus sakei</i>
starter cultures
probiotics
bacteriocins
safety
virulence factors
Online Access:https://www.mdpi.com/2304-8158/13/23/3770
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Summary:This work aimed to evaluate some of the probiotic features and safety of the bacteriocin-producing <i>Latilactobacillus sakei</i> subsp. <i>sakei</i> 2a. The effect of selected commercial drugs from different generic groups and antibiotics on the growth of <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a was also determined. The presence of virulence factors was determined based on PCR with total DNA from <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a. Good growth of <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a was recorded in MRS broth supplemented with 0.2% or 0.4% oxbile or in MRS broth adjusted to a pH from 5.0–9.0. Auto-aggregation of <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a was 62.59%. Different levels of co-aggregation were recorded between <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a and <i>Enterococcus faecalis</i> ATCC19443, <i>Ltb. sakei</i> ATCC15521 and <i>Listeria monocytogenes</i> ScottA. Growth of <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a was not inhibited by commercial drugs from different generic groups. The inhibitory effect on the growth of <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a was recorded only in the presence of Arotin [selective serotonin reuptake inhibitor antidepressant] Minimal Inhibition Concentration (MIC) 1.0 mg/mL, Atlansil [Antiarrhythmic] MIC 0.625 mg/mL, Diclofenac potassium [non-steroidal anti-inflammatory drug (NSAID)] MIC 2.5 mg/mL and Spidufen [NSAID] MIC 15.0 mg/mL. Only two antibiotics tested in this study, Amoxil and Urotrobel, inhibited the growth of <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a with a MIC of <0.5 mg/mL and 5.0 mg/mL, respectively. However, <i>Ltb. sakei</i> subsp. <i>sakei</i> 2a generated positive PCR results on the DNA level for <i>van</i>A (vancomycin resistance), <i>hyl</i> (hyaluronidase), <i>esp</i> (enterococcal surface protein), <i>ace</i> (adhesion of collagen) and <i>cil</i>A (cytolisin) and a high virulence profile when examined for the presence of virulence factors. It is important to underline that cytolysis has been described as a virulence and antibacterial factor.
ISSN:2304-8158

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