Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS
Salvianolic acid B is the main active ingredient in salvianolate injection, which is produced by extracting danshen, the most commonly used Chinese herbal medicine for cardiovascular treatment. Clinically, salvianolate injection and aspirin are commonly combined to treat coronary heart diseases in p...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/6620868 |
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| author | Weiyi Cao Hezhi Yao Zhenzhen Qian Yi Wu Shuge Wang Rui Li Rui Gao |
| author_facet | Weiyi Cao Hezhi Yao Zhenzhen Qian Yi Wu Shuge Wang Rui Li Rui Gao |
| author_sort | Weiyi Cao |
| collection | DOAJ |
| description | Salvianolic acid B is the main active ingredient in salvianolate injection, which is produced by extracting danshen, the most commonly used Chinese herbal medicine for cardiovascular treatment. Clinically, salvianolate injection and aspirin are commonly combined to treat coronary heart diseases in patients with stable angina. To support clinical studies on drug-drug interactions (DDIs) between salvianolate injection and aspirin, a rapid and sensitive UPLC-MS/MS method for the simultaneous determination of aspirin (acetylsalicylic acid), its metabolite salicylic acid, and salvianolic acid B in human plasma was developed. The analytes and internal standard were extracted from the acidified plasma by liquid-liquid extraction with ethyl acetate and then separated by gradient elution with acetonitrile/0.5% formic acid in water on a C18 column. Salvianolic acid B, acetylsalicylic acid, and salicylic acid were quantified in multiple-reaction monitoring mode with negative ion electrospray ionization. The method was fully validated according to the current regulatory guidance for bioanalysis. Calibration curves in the range 5–6000 ng/mL, all with correlation coefficients greater than 0.99, were established using linear regression models for salvianolic acid B and acetylsalicylic acid and a quadratic model for salicylic acid. The validated method was successfully used to measure salvianolic acid B, acetylsalicylic acid, and salicylic acid concentrations in human plasma samples from 16 patients to observe the pharmacokinetic changes caused by DDIs. |
| format | Article |
| id | doaj-art-4b6441deff95430fb12bbcb8eb56956a |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-4b6441deff95430fb12bbcb8eb56956a2025-02-03T01:05:19ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792021-01-01202110.1155/2021/66208686620868Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MSWeiyi Cao0Hezhi Yao1Zhenzhen Qian2Yi Wu3Shuge Wang4Rui Li5Rui Gao6Institute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaInstitute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaInstitute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaInstitute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaInstitute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaInstitute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaInstitute of Clinical Pharmacology of Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1, R. Xiyuangcaochang, Haidian District, Beijing 100091, ChinaSalvianolic acid B is the main active ingredient in salvianolate injection, which is produced by extracting danshen, the most commonly used Chinese herbal medicine for cardiovascular treatment. Clinically, salvianolate injection and aspirin are commonly combined to treat coronary heart diseases in patients with stable angina. To support clinical studies on drug-drug interactions (DDIs) between salvianolate injection and aspirin, a rapid and sensitive UPLC-MS/MS method for the simultaneous determination of aspirin (acetylsalicylic acid), its metabolite salicylic acid, and salvianolic acid B in human plasma was developed. The analytes and internal standard were extracted from the acidified plasma by liquid-liquid extraction with ethyl acetate and then separated by gradient elution with acetonitrile/0.5% formic acid in water on a C18 column. Salvianolic acid B, acetylsalicylic acid, and salicylic acid were quantified in multiple-reaction monitoring mode with negative ion electrospray ionization. The method was fully validated according to the current regulatory guidance for bioanalysis. Calibration curves in the range 5–6000 ng/mL, all with correlation coefficients greater than 0.99, were established using linear regression models for salvianolic acid B and acetylsalicylic acid and a quadratic model for salicylic acid. The validated method was successfully used to measure salvianolic acid B, acetylsalicylic acid, and salicylic acid concentrations in human plasma samples from 16 patients to observe the pharmacokinetic changes caused by DDIs.http://dx.doi.org/10.1155/2021/6620868 |
| spellingShingle | Weiyi Cao Hezhi Yao Zhenzhen Qian Yi Wu Shuge Wang Rui Li Rui Gao Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS International Journal of Analytical Chemistry |
| title | Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS |
| title_full | Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS |
| title_fullStr | Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS |
| title_full_unstemmed | Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS |
| title_short | Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS |
| title_sort | simultaneous quantification of aspirin its metabolite salicylic acid and salvianolic acid b in human plasma using uplc ms ms |
| url | http://dx.doi.org/10.1155/2021/6620868 |
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