Pretreatment of maize straw with Pleurotus ostreatus and Lentinus squarrosulus for bioethanol production using Saccharomyces cerevisiae
Maize straw (MS) is a lignocellulosic substrate that constitutes huge wastes in the environment. This work aimed to pretreat MS with mushroom alone as a biological agent, and with NaOH prior to mushroom treatment (combined chemical and biological), and subsequently converting the released reducin...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2021-12-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_209731_213d530713d7b4ecc92a472f48cbc8d3.pdf |
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| Summary: | Maize straw (MS) is a lignocellulosic substrate that constitutes huge wastes in the
environment. This work aimed to pretreat MS with mushroom alone as a biological agent, and
with NaOH prior to mushroom treatment (combined chemical and biological), and
subsequently converting the released reducing sugars (RS) to ethanol using Saccharomyces
cerevisiae. MS was degraded by Pleurotus ostreatus (PO) and Lentinus squarrosulus singly
and in combination for 35 d. Samples were collected every 7 d from the treated straw to
determine the RS content. Moreover, MS samples were pretreated with NaOH prior to
degradation by the selected mushroom (combined pretreatment), and then their sugar profiles
were determined using High-performance liquid chromatography (HPLC). The RS recovered
from the degraded MS samples were fermented using 2 molecularly-identified S. cerevisiae
strains. The highest RS contents (16.79 mg/ g) were recorded when MS was pre-degraded by
PO for 21 d compared to Lentinus squarrosulus (16.55 mg/ g), and with the consortium of the
two fungal cultures (16.36 mg/ g). However, MS pretreated with NaOH and Pleurotus
ostreatus gave better yield of RS (17.38 mg/ g), than treatment with Pleurotus ostreatus (16.79
mg/ g) alone. The sugar profiles of the NaOH-PO-pretreated MS (mg/ 100 g) included;
glucose (850.60); xylose (837.04), fructose (754.29), arabinose (502.76), ribose (2.066×10-4
)
and rhamnose (3.552×10-5
). The fermenting yeasts were molecularly identified by sequencing
of ITS region as S. cerevisiae SA01 and S. cerevisiae SA02, and assigned Accession no. of
MK038975 and MN491900, respectively. Equal concentration of bioethanol (1.58 g/ l) was
recorded in PO and in NaOH-PO-pretreated MS, which were fermented by S. cerevisiae
SA01. Accordingly, MS can be utilized as a substrate for fermentation and then bioethanol
production. |
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| ISSN: | 2537-0286 2537-0294 |