Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration
Articular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting chondrocyte growth and cartilage regeneration, but its delivery has been limited to injections. Continuous PDRN deliv...
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2025-01-01
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| author | Sunjae Park Youngjun Son Jonggyu Park Soyoon Lee Na-Hyeon Kim Se-Na Jang Tae-Woong Kang Jeong-Eun Song Gilson Khang |
| author_facet | Sunjae Park Youngjun Son Jonggyu Park Soyoon Lee Na-Hyeon Kim Se-Na Jang Tae-Woong Kang Jeong-Eun Song Gilson Khang |
| author_sort | Sunjae Park |
| collection | DOAJ |
| description | Articular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting chondrocyte growth and cartilage regeneration, but its delivery has been limited to injections. Continuous PDRN delivery is crucial for effective cartilage regeneration. This study explores using gelatin methacrylate (gelMA) hydrogel, crosslinked with visible light and riboflavin 5′-phosphate sodium (RF) as a photoinitiator, for sustained PDRN release. GelMA hydrogel’s synthesis was confirmed through spectrophotometric techniques, demonstrating successful methacrylate group incorporation. PDRN-loaded gelMA hydrogels displayed varying pore sizes, swelling ratios, degradation rates, and mechanical properties based on gelMA concentration. They showed sustained PDRN release and biocompatibility, with the 14% gelMA-PDRN composition performing best. Glycosaminoglycan (GAG) activity was higher in PDRN-loaded hydrogels, indicating a positive effect on cartilage formation. RT-PCR analysis revealed increased expression of cartilage-specific genes (COL2, SOX9, AGG) in gelMA-PDRN. Histological assessments in a rabbit cartilage defect model demonstrated superior regenerative effects of gelMA-PDRN hydrogels. This study highlights the potential of gelMA-PDRN hydrogels in cartilage tissue engineering, providing a promising approach for effective cartilage regeneration. |
| format | Article |
| id | doaj-art-4b2aee67edde4890bfb7f97603508df2 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Gels |
| spelling | doaj-art-4b2aee67edde4890bfb7f97603508df22025-01-24T13:33:53ZengMDPI AGGels2310-28612025-01-011114210.3390/gels11010042Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage RegenerationSunjae Park0Youngjun Son1Jonggyu Park2Soyoon Lee3Na-Hyeon Kim4Se-Na Jang5Tae-Woong Kang6Jeong-Eun Song7Gilson Khang8Department of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaArticular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting chondrocyte growth and cartilage regeneration, but its delivery has been limited to injections. Continuous PDRN delivery is crucial for effective cartilage regeneration. This study explores using gelatin methacrylate (gelMA) hydrogel, crosslinked with visible light and riboflavin 5′-phosphate sodium (RF) as a photoinitiator, for sustained PDRN release. GelMA hydrogel’s synthesis was confirmed through spectrophotometric techniques, demonstrating successful methacrylate group incorporation. PDRN-loaded gelMA hydrogels displayed varying pore sizes, swelling ratios, degradation rates, and mechanical properties based on gelMA concentration. They showed sustained PDRN release and biocompatibility, with the 14% gelMA-PDRN composition performing best. Glycosaminoglycan (GAG) activity was higher in PDRN-loaded hydrogels, indicating a positive effect on cartilage formation. RT-PCR analysis revealed increased expression of cartilage-specific genes (COL2, SOX9, AGG) in gelMA-PDRN. Histological assessments in a rabbit cartilage defect model demonstrated superior regenerative effects of gelMA-PDRN hydrogels. This study highlights the potential of gelMA-PDRN hydrogels in cartilage tissue engineering, providing a promising approach for effective cartilage regeneration.https://www.mdpi.com/2310-2861/11/1/42cartilage regenerationGelMA hydrogelPDRNvisible light crosslinkingbiocompatibilitytissue engineering |
| spellingShingle | Sunjae Park Youngjun Son Jonggyu Park Soyoon Lee Na-Hyeon Kim Se-Na Jang Tae-Woong Kang Jeong-Eun Song Gilson Khang Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration Gels cartilage regeneration GelMA hydrogel PDRN visible light crosslinking biocompatibility tissue engineering |
| title | Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration |
| title_full | Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration |
| title_fullStr | Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration |
| title_full_unstemmed | Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration |
| title_short | Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration |
| title_sort | polydeoxynucleotide loaded visible light photo crosslinked gelatin methacrylate hydrogel approach to accelerating cartilage regeneration |
| topic | cartilage regeneration GelMA hydrogel PDRN visible light crosslinking biocompatibility tissue engineering |
| url | https://www.mdpi.com/2310-2861/11/1/42 |
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