HBx promotes glomerular podocyte-induced immune cell responses
Background Podocytes, as intrinsic renal cells, can also express MHC-II and costimulatory molecules under inflammatory conditions, suggesting that they may act as antigen-presenting cells (APCs) to activate immune cell responses and then lead to immune-mediated renal injury. They are already recogni...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2373276 |
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| author | Luyan Bian Yuchao Niu Weijie Yuan Huasheng Du Yitong Yang |
| author_facet | Luyan Bian Yuchao Niu Weijie Yuan Huasheng Du Yitong Yang |
| author_sort | Luyan Bian |
| collection | DOAJ |
| description | Background Podocytes, as intrinsic renal cells, can also express MHC-II and costimulatory molecules under inflammatory conditions, suggesting that they may act as antigen-presenting cells (APCs) to activate immune cell responses and then lead to immune-mediated renal injury. They are already recognized as main targets in the pathogenic mechanism of hepatitis B virus (HBV)-associated glomerulonephritis (HBV-GN). Previous studies also have indicated that inflammatory cells infiltration and immune-mediated tissue injury are evident in the kidney samples of patients with HBV-GN. However, the role of podocytes immune disorder in the pathogenic mechanism of HBV-GN remains unclear.Methods Renal function and inflammatory cells infiltration were measured in HBV transgenic (HBV-Tg) mice. In vitro, podocytes/CD4+ T cells or macrophages co-culture system was established. Then, the expression of HBx, CD4, and CD68 was determined by immunohistochemistry, while the expression of MHC-II, CD40, and CD40L was determined by immunofluorescence. Co-stimulatory molecules expression was examined by flow cytometry. The levels of inflammatory factors were detected by ELISA.Results In vivo, renal function was obviously impaired in HBV-Tg mice. HBx was significantly upregulated and immune cells infiltrated in the glomerulus of HBV-Tg mice. Expression of MHC-II and costimulatory molecule CD40 increased in the podocytes of HBV-Tg mice; CD4+ T cells exhibited increased CD40L expression in glomerulus. In vitro, CD40 expression was markedly elevated in HBx-podocytes. In co-culture systems, HBx-podocytes stimulated CD4+ T cells activation and caused the imbalance between IFN-γ and IL-4. HBx-podocytes also enhanced the adhesion ability of macrophages and induced the release of proinflammatory mediators.Conclusion Taken together, these podocyte-related immune disorder may be involved in the pathogenic mechanism of HBV-GN. |
| format | Article |
| id | doaj-art-4b1e3a3b5d1f45619ad053aada304cbb |
| institution | DOAJ |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-4b1e3a3b5d1f45619ad053aada304cbb2025-08-20T03:21:30ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146210.1080/0886022X.2024.2373276HBx promotes glomerular podocyte-induced immune cell responsesLuyan Bian0Yuchao Niu1Weijie Yuan2Huasheng Du3Yitong Yang4Department of Nephrology, Qingdao Municipal Hospital, University of Health and Rehabilitation Sciences, Qingdao, ChinaDepartment of Oncology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences, Qingdao, ChinaDepartment of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University School, Shanghai, ChinaDepartment of Nephrology, Qingdao Municipal Hospital, University of Health and Rehabilitation Sciences, Qingdao, ChinaDepartment of Nephrology, Qingdao Municipal Hospital, University of Health and Rehabilitation Sciences, Qingdao, ChinaBackground Podocytes, as intrinsic renal cells, can also express MHC-II and costimulatory molecules under inflammatory conditions, suggesting that they may act as antigen-presenting cells (APCs) to activate immune cell responses and then lead to immune-mediated renal injury. They are already recognized as main targets in the pathogenic mechanism of hepatitis B virus (HBV)-associated glomerulonephritis (HBV-GN). Previous studies also have indicated that inflammatory cells infiltration and immune-mediated tissue injury are evident in the kidney samples of patients with HBV-GN. However, the role of podocytes immune disorder in the pathogenic mechanism of HBV-GN remains unclear.Methods Renal function and inflammatory cells infiltration were measured in HBV transgenic (HBV-Tg) mice. In vitro, podocytes/CD4+ T cells or macrophages co-culture system was established. Then, the expression of HBx, CD4, and CD68 was determined by immunohistochemistry, while the expression of MHC-II, CD40, and CD40L was determined by immunofluorescence. Co-stimulatory molecules expression was examined by flow cytometry. The levels of inflammatory factors were detected by ELISA.Results In vivo, renal function was obviously impaired in HBV-Tg mice. HBx was significantly upregulated and immune cells infiltrated in the glomerulus of HBV-Tg mice. Expression of MHC-II and costimulatory molecule CD40 increased in the podocytes of HBV-Tg mice; CD4+ T cells exhibited increased CD40L expression in glomerulus. In vitro, CD40 expression was markedly elevated in HBx-podocytes. In co-culture systems, HBx-podocytes stimulated CD4+ T cells activation and caused the imbalance between IFN-γ and IL-4. HBx-podocytes also enhanced the adhesion ability of macrophages and induced the release of proinflammatory mediators.Conclusion Taken together, these podocyte-related immune disorder may be involved in the pathogenic mechanism of HBV-GN.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2373276HBxpodocytesCD4+ T cellsmacrophagesimmune responsesinflammatory responses |
| spellingShingle | Luyan Bian Yuchao Niu Weijie Yuan Huasheng Du Yitong Yang HBx promotes glomerular podocyte-induced immune cell responses Renal Failure HBx podocytes CD4+ T cells macrophages immune responses inflammatory responses |
| title | HBx promotes glomerular podocyte-induced immune cell responses |
| title_full | HBx promotes glomerular podocyte-induced immune cell responses |
| title_fullStr | HBx promotes glomerular podocyte-induced immune cell responses |
| title_full_unstemmed | HBx promotes glomerular podocyte-induced immune cell responses |
| title_short | HBx promotes glomerular podocyte-induced immune cell responses |
| title_sort | hbx promotes glomerular podocyte induced immune cell responses |
| topic | HBx podocytes CD4+ T cells macrophages immune responses inflammatory responses |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2373276 |
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