Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro
Milk allergy is a common allergic reaction found in infants and young children, most of them appear tolerance after growing up. In this study, infant formula was digested by simulated in vitro digestion method. The potential non-allergenic peptides were further screened from undigested products by e...
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002075 |
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| author | Zihao Xu Hao Bai Xin Ma Yong Wu Zhihua Wu Anshu Yang Weixiang Mao Xin Li Hongbing Chen |
| author_facet | Zihao Xu Hao Bai Xin Ma Yong Wu Zhihua Wu Anshu Yang Weixiang Mao Xin Li Hongbing Chen |
| author_sort | Zihao Xu |
| collection | DOAJ |
| description | Milk allergy is a common allergic reaction found in infants and young children, most of them appear tolerance after growing up. In this study, infant formula was digested by simulated in vitro digestion method. The potential non-allergenic peptides were further screened from undigested products by exclusion of the known epitopes from β-lactoglobulin (BLG) and α-lactalbumin (ALA). These potential non-allergenic peptides were synthesized and their transferability were determined by Caco-2 cell monolayer model. Finally, the potential allergenicity were evaluated by KU812 cell degranulation model. The results showed that 7 peptides were screened as non-allergenic sequences, among which were 3 from ALA and 4 from BLG. The Caco-2 cell model showed that all the synthetic peptides were absorbed and transported well. However, only peptide BLG107-118 showed potential allegencity by KU812 model. In conclusion, six peptides, including ALA29–51, ALA80–90, ALA94–103, BLG1–20, BLG24–50, and BLG123–139 were evaluated as hypoallergenic peptides, which could be used for candidates of peptides inducing immune tolerance for persons with milk allergy. |
| format | Article |
| id | doaj-art-4b0eddb1561e4fdf8b503157a1c1478a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-4b0eddb1561e4fdf8b503157a1c1478a2025-02-03T10:34:08ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123817824Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitroZihao Xu0Hao Bai1Xin Ma2Yong Wu3Zhihua Wu4Anshu Yang5Weixiang Mao6Xin Li7Hongbing Chen8State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, ChinaState Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, ChinaState Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, ChinaJiangxi Institute of Quality and Standardization, Nanchang 330046, China; Corresponding authors.State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China; Corresponding authors.State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, ChinaMilk allergy is a common allergic reaction found in infants and young children, most of them appear tolerance after growing up. In this study, infant formula was digested by simulated in vitro digestion method. The potential non-allergenic peptides were further screened from undigested products by exclusion of the known epitopes from β-lactoglobulin (BLG) and α-lactalbumin (ALA). These potential non-allergenic peptides were synthesized and their transferability were determined by Caco-2 cell monolayer model. Finally, the potential allergenicity were evaluated by KU812 cell degranulation model. The results showed that 7 peptides were screened as non-allergenic sequences, among which were 3 from ALA and 4 from BLG. The Caco-2 cell model showed that all the synthetic peptides were absorbed and transported well. However, only peptide BLG107-118 showed potential allegencity by KU812 model. In conclusion, six peptides, including ALA29–51, ALA80–90, ALA94–103, BLG1–20, BLG24–50, and BLG123–139 were evaluated as hypoallergenic peptides, which could be used for candidates of peptides inducing immune tolerance for persons with milk allergy.http://www.sciencedirect.com/science/article/pii/S2213453022002075Milk allergyEpitopesPeptidesHypoallergenicImmune tolerance |
| spellingShingle | Zihao Xu Hao Bai Xin Ma Yong Wu Zhihua Wu Anshu Yang Weixiang Mao Xin Li Hongbing Chen Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro Food Science and Human Wellness Milk allergy Epitopes Peptides Hypoallergenic Immune tolerance |
| title | Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro |
| title_full | Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro |
| title_fullStr | Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro |
| title_full_unstemmed | Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro |
| title_short | Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro |
| title_sort | cytological evaluation by caco 2 and ku812 of non allergenic peptides from simulated digestion of infant formula in vitro |
| topic | Milk allergy Epitopes Peptides Hypoallergenic Immune tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002075 |
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