Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue
Excess ammonia can damage the growth and development of fish. <i>Pseudobagrus ussuriensis</i> is a scaleless fish with important economic value that is more sensitive to ammonia stress. In this study, <i>P. ussuriensis</i> was explored using different ammonia concentrations [...
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2025-01-01
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| author | Shun Shi Xiaohui Sun Chunnuan Zhang Chenran Lv Yajuan Liu Juan Du Qian Qi |
| author_facet | Shun Shi Xiaohui Sun Chunnuan Zhang Chenran Lv Yajuan Liu Juan Du Qian Qi |
| author_sort | Shun Shi |
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| description | Excess ammonia can damage the growth and development of fish. <i>Pseudobagrus ussuriensis</i> is a scaleless fish with important economic value that is more sensitive to ammonia stress. In this study, <i>P. ussuriensis</i> was explored using different ammonia concentrations [control (0 mg/L), CL; low stress (10 mg/L), T1L; and high stress (50 mg/L), T2L] for 48 h. Compared to the control group, the liver cells in the T1L group showed slight damage, while the T2L group was severely damaged, with the cells being loosely arranged, with nuclei lysis and cell vacuolization. The activities of superoxide dismutase, catalase, and glutathione in the T1L and T2L groups were significantly lower than those in the CL group (<i>p</i> < 0.05), and the malondialdehyde reached the maximum at 48 h. Furthermore, 9301 differentially expressed genes (DEGs) (4583 upregulated and 4718 downregulated) were detected by transcriptome sequencing. Most DEGs were highly enriched in cellular processes (GO:0009987) and cell parts (GO:0044464). Especially, the phosphatidylinositol 3-kinase/protein kinase B (PI3K-Akt) signaling pathway had the maximum quantity of DEGs in all the three groups. In-depth analysis revealed the stress caused multiple substitutions of SNP sites in pik3ca and kras, blocking the PI3K/Akt signaling pathway to prevent cancer cell proliferation and spread, accelerating the apoptosis of damaged cells. These results suggest that ammonia stress induces liver damage in <i>P. ussuriensis</i>, causing genetic mutations and cellular carcinogenesis, thereby accelerating cell apoptosis. |
| format | Article |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-4b041aa96a2a4ce3b8d2aed829a4777c2025-01-24T13:32:27ZengMDPI AGFishes2410-38882025-01-011011710.3390/fishes10010017Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver TissueShun Shi0Xiaohui Sun1Chunnuan Zhang2Chenran Lv3Yajuan Liu4Juan Du5Qian Qi6College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, ChinaLuoyang Agricultural Technology Extension Service Center, Luoyang 471003, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, ChinaLuoyang Agricultural Technology Extension Service Center, Luoyang 471003, ChinaLuoyang Agricultural Technology Extension Service Center, Luoyang 471003, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, ChinaExcess ammonia can damage the growth and development of fish. <i>Pseudobagrus ussuriensis</i> is a scaleless fish with important economic value that is more sensitive to ammonia stress. In this study, <i>P. ussuriensis</i> was explored using different ammonia concentrations [control (0 mg/L), CL; low stress (10 mg/L), T1L; and high stress (50 mg/L), T2L] for 48 h. Compared to the control group, the liver cells in the T1L group showed slight damage, while the T2L group was severely damaged, with the cells being loosely arranged, with nuclei lysis and cell vacuolization. The activities of superoxide dismutase, catalase, and glutathione in the T1L and T2L groups were significantly lower than those in the CL group (<i>p</i> < 0.05), and the malondialdehyde reached the maximum at 48 h. Furthermore, 9301 differentially expressed genes (DEGs) (4583 upregulated and 4718 downregulated) were detected by transcriptome sequencing. Most DEGs were highly enriched in cellular processes (GO:0009987) and cell parts (GO:0044464). Especially, the phosphatidylinositol 3-kinase/protein kinase B (PI3K-Akt) signaling pathway had the maximum quantity of DEGs in all the three groups. In-depth analysis revealed the stress caused multiple substitutions of SNP sites in pik3ca and kras, blocking the PI3K/Akt signaling pathway to prevent cancer cell proliferation and spread, accelerating the apoptosis of damaged cells. These results suggest that ammonia stress induces liver damage in <i>P. ussuriensis</i>, causing genetic mutations and cellular carcinogenesis, thereby accelerating cell apoptosis.https://www.mdpi.com/2410-3888/10/1/17<i>Pseudobagrus ussuriensis</i>acute ammonia stressliver tissuetranscriptome characteristics |
| spellingShingle | Shun Shi Xiaohui Sun Chunnuan Zhang Chenran Lv Yajuan Liu Juan Du Qian Qi Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue Fishes <i>Pseudobagrus ussuriensis</i> acute ammonia stress liver tissue transcriptome characteristics |
| title | Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue |
| title_full | Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue |
| title_fullStr | Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue |
| title_full_unstemmed | Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue |
| title_short | Transcriptome Analysis of the Effect of Acute Ammonia Stress on <i>Pseudobagrus ussuriensis</i> Liver Tissue |
| title_sort | transcriptome analysis of the effect of acute ammonia stress on i pseudobagrus ussuriensis i liver tissue |
| topic | <i>Pseudobagrus ussuriensis</i> acute ammonia stress liver tissue transcriptome characteristics |
| url | https://www.mdpi.com/2410-3888/10/1/17 |
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