The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats
Aim The aim of the present study was to produce chrysin–curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin–curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.Materials and methods The ele...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-12-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1594855 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Aim The aim of the present study was to produce chrysin–curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin–curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.Materials and methods The electrospinning method was carried out for the preparation of the chrysin, curcumin and chrysin–curcumin-loaded PCL-PEG nanofibres with different concentrations. FTIR and SEM were performed to characterize the chemical structures and morphology of the nanofibres. In vitro drug release, as well as in vivo wound-healing studies were investigated in male rats. The expressions of genes related to the wound-healing process were also evaluated by real-time PCR.Results Our study showed that the chrysin–curcumin-loaded nanofibres have anti-inflammatory properties in several stages of the wound-healing process by affecting the IL-6, MMP-2, TIMP-1, TIMP-2 and iNOS gene expression. Our results demonstrated that the effect of the chrysin-loaded nanofibre, the curcumin-loaded nanofibre and the chrysin–curcumin-loaded nanofibre in the wound-healing process is dose dependent and in accordance with the obtained results in that it might affect the inflammation phase more than the other stages of the wound-healing process.Conclusion We have introduced chrysin–curcumin-loaded PCL-PEG nanofibres as a novel compound for shortening the duration of the wound-healing process. |
|---|---|
| ISSN: | 2169-1401 2169-141X |