Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation
On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlati...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/659862 |
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| author | J. M. Morrell A. Johannisson L. Juntilla K. Rytty L. Bäckgren A.-M. Dalin H. Rodriguez-Martinez |
| author_facet | J. M. Morrell A. Johannisson L. Juntilla K. Rytty L. Bäckgren A.-M. Dalin H. Rodriguez-Martinez |
| author_sort | J. M. Morrell |
| collection | DOAJ |
| description | On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chamber (𝑟=0.84; 𝑃<.001
). Similarly, there was a good correlation between sperm viability results from the Nucleocounter SP-100 and flow cytometric results (𝑟=0.73; 𝑃<.001). Sperm viability at 24 hours was significantly better for samples extended in INRA96 than in Kenney's extender (𝑃<.001). Furthermore, sperm kinematics were better for stored samples in INRA96 than in Kenney's extender. Single Layer Centrifugation selected spermatozoa that maintained their viability better during storage for 24 hours than the uncentrifuged samples. In conclusion, the type of semen extender used and Single Layer Centrifugation were found to influence both the kinematics and viability of stallion spermatozoa. The Nucelocounter-SP100 was considered to be a useful instrument for rapidly measuring stallion sperm concentration and viability. |
| format | Article |
| id | doaj-art-4ae020e69c08423fb3195b5aa5e0447f |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-4ae020e69c08423fb3195b5aa5e0447f2025-02-03T05:59:16ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/659862659862Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer CentrifugationJ. M. Morrell0A. Johannisson1L. Juntilla2K. Rytty3L. Bäckgren4A.-M. Dalin5H. Rodriguez-Martinez6Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, SwedenDepartment of Anatomy, Physiology & Biochemistry, Swedish University of Agricultural Sciences (SLU), P.O. Box 7011, 75007 Uppsala, SwedenDivision of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, SwedenDivision of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, SwedenDepartment of Anatomy, Physiology & Biochemistry, Swedish University of Agricultural Sciences (SLU), P.O. Box 7011, 75007 Uppsala, SwedenDivision of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, SwedenDivision of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, SwedenOn-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chamber (𝑟=0.84; 𝑃<.001 ). Similarly, there was a good correlation between sperm viability results from the Nucleocounter SP-100 and flow cytometric results (𝑟=0.73; 𝑃<.001). Sperm viability at 24 hours was significantly better for samples extended in INRA96 than in Kenney's extender (𝑃<.001). Furthermore, sperm kinematics were better for stored samples in INRA96 than in Kenney's extender. Single Layer Centrifugation selected spermatozoa that maintained their viability better during storage for 24 hours than the uncentrifuged samples. In conclusion, the type of semen extender used and Single Layer Centrifugation were found to influence both the kinematics and viability of stallion spermatozoa. The Nucelocounter-SP100 was considered to be a useful instrument for rapidly measuring stallion sperm concentration and viability.http://dx.doi.org/10.4061/2010/659862 |
| spellingShingle | J. M. Morrell A. Johannisson L. Juntilla K. Rytty L. Bäckgren A.-M. Dalin H. Rodriguez-Martinez Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation Veterinary Medicine International |
| title | Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation |
| title_full | Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation |
| title_fullStr | Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation |
| title_full_unstemmed | Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation |
| title_short | Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation |
| title_sort | stallion sperm viability as measured by the nucleocounter sp 100 is affected by extender and enhanced by single layer centrifugation |
| url | http://dx.doi.org/10.4061/2010/659862 |
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