A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization

A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization

Abstract Gene expression profiling is of key importance in all domains of life sciences, as medicine, environment, and plants, for both basic and applied research. Despite the emergence of microarrays and high-throughput sequencing, qPCR remains a standard method for gene expression analyses, with i...

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Bibliographic Details
Main Authors: Anis Djari, Guillaume Madignier, Christian Chervin, Benoît van der Rest, James J. Giovannoni, Mondher Bouzayen, Julien Pirrello, Elie Maza
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-82651-w
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