The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis)
Cold stress widely impairs the quality and yield of tea plants. The miR164 family and its target NAC transcription factor have been identified as crucial regulators in response to cold stress. However, the role of miR164 and CsNAC in cold tolerance in tea plants was little understood. In our study,...
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KeAi Communications Co., Ltd.
2025-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311924004428 |
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| author | Siya Li Lu Cao Ziwen Zhou Yaohua Cheng Xianchen Zhang Yeyun Li |
| author_facet | Siya Li Lu Cao Ziwen Zhou Yaohua Cheng Xianchen Zhang Yeyun Li |
| author_sort | Siya Li |
| collection | DOAJ |
| description | Cold stress widely impairs the quality and yield of tea plants. The miR164 family and its target NAC transcription factor have been identified as crucial regulators in response to cold stress. However, the role of miR164 and CsNAC in cold tolerance in tea plants was little understood. In our study, the expression level of CsMIR164a was significantly reduced under cold stress and significantly and negatively correlated with that of CsNAC1. 5´ RACE and GUS histochemical assays showed that CsNAC1 was cleaved by CsMIR164a. The CsMIR164a-silenced tea leaves promoted the expression levels of CsNAC1 and CsCBFs and exhibited greater cold tolerance. Also, the overexpression of CsNAC1 enhanced cold tolerance in transgenic Arabidopsis plants by promoting the expression levels of AtCBFs. In contrast, the heterologous overexpression of CsMIR164a in Arabidopsis decreased the expression level of AtNACs and AtCBFs and thus impaired cold tolerance. Additionally, silencing of CsNAC1 impaired the expression levels of CsCBFs, resulting in greater cold sensitivity in tea leaves. Our present study demonstrated that the miR164a-CsNAC1 module may play a negative role in the cold tolerance of tea plants via the CsCBF-dependent pathway. |
| format | Article |
| id | doaj-art-4aa7945b8dfc4f2a8b273b384f56ca15 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-4aa7945b8dfc4f2a8b273b384f56ca152025-08-20T03:58:10ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-08-012483073308610.1016/j.jia.2024.12.033The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis)Siya Li0Lu Cao1Ziwen Zhou2Yaohua Cheng3Xianchen Zhang4Yeyun Li5State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, ChinaCorrespondence Xianchen Zhang; State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, ChinaCorrespondence Yeyun Li; State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, ChinaCold stress widely impairs the quality and yield of tea plants. The miR164 family and its target NAC transcription factor have been identified as crucial regulators in response to cold stress. However, the role of miR164 and CsNAC in cold tolerance in tea plants was little understood. In our study, the expression level of CsMIR164a was significantly reduced under cold stress and significantly and negatively correlated with that of CsNAC1. 5´ RACE and GUS histochemical assays showed that CsNAC1 was cleaved by CsMIR164a. The CsMIR164a-silenced tea leaves promoted the expression levels of CsNAC1 and CsCBFs and exhibited greater cold tolerance. Also, the overexpression of CsNAC1 enhanced cold tolerance in transgenic Arabidopsis plants by promoting the expression levels of AtCBFs. In contrast, the heterologous overexpression of CsMIR164a in Arabidopsis decreased the expression level of AtNACs and AtCBFs and thus impaired cold tolerance. Additionally, silencing of CsNAC1 impaired the expression levels of CsCBFs, resulting in greater cold sensitivity in tea leaves. Our present study demonstrated that the miR164a-CsNAC1 module may play a negative role in the cold tolerance of tea plants via the CsCBF-dependent pathway.http://www.sciencedirect.com/science/article/pii/S2095311924004428tea plantscold stressmiRNAsNAC transcription factorfunctional verification |
| spellingShingle | Siya Li Lu Cao Ziwen Zhou Yaohua Cheng Xianchen Zhang Yeyun Li The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis) Journal of Integrative Agriculture tea plants cold stress miRNAs NAC transcription factor functional verification |
| title | The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis) |
| title_full | The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis) |
| title_fullStr | The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis) |
| title_full_unstemmed | The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis) |
| title_short | The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants (Camellia sinensis) |
| title_sort | mir164a targets csnac1 to negatively regulate the cold tolerance of tea plants camellia sinensis |
| topic | tea plants cold stress miRNAs NAC transcription factor functional verification |
| url | http://www.sciencedirect.com/science/article/pii/S2095311924004428 |
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