Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines
Purpose: Evaluate the integrity, reproducibility, and image quality of total retinal thickness (TRT) measurements between two generations of Spectralis Spectral Domain Optical Coherence Tomography (SD-OCT) instruments (Old OCT: 2011, New OCT: 2017). Design: Prospective cohort study evaluating TRT me...
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Elsevier
2025-04-01
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| Series: | AJO International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950253525000024 |
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| author | Kyoung A Viola Lee Corey Tesdahl Keith Zimmerman Kimberly Jun Sabrina Khalil Alexander Shahin Abdullah Abou-Samra Ramesh Ayyala Radouil Tzekov |
| author_facet | Kyoung A Viola Lee Corey Tesdahl Keith Zimmerman Kimberly Jun Sabrina Khalil Alexander Shahin Abdullah Abou-Samra Ramesh Ayyala Radouil Tzekov |
| author_sort | Kyoung A Viola Lee |
| collection | DOAJ |
| description | Purpose: Evaluate the integrity, reproducibility, and image quality of total retinal thickness (TRT) measurements between two generations of Spectralis Spectral Domain Optical Coherence Tomography (SD-OCT) instruments (Old OCT: 2011, New OCT: 2017). Design: Prospective cohort study evaluating TRT measurements across two visits. Subjects and participants: Fourteen healthy individuals (28 eyes, age range: 22-54 years) underwent TRT measurements using both Old and New OCT models, with each eye receiving three consecutive scans per visit. Methods and testing: TRT measurements were performed using the Posterior Pole Algorithm (PPA) and Early Treatment Diabetic Retinopathy Study (ETDRS) grid protocols. Reproducibility was evaluated using Average Pairwise Pearson Correlation (APPC), while image quality was measured by Signal-to-Noise Ratio (SNR) and Contrast-to-Noise Ratio (CNR). Agreement between the devices was analyzed through Bland-Altman plots, and spatial variability was visualized using heatmaps. The dimensionality reduction techniques Principal Component Analysis (PCA) and Multidimensional Scaling (MDS) were employed to explore data patterns. Main outcome measures: Reproducibility of TRT measurements, image quality, and the degree of agreement between the two OCT models. Results: Both the Old and New OCT models demonstrated high reproducibility (APPC: 0.995-0.998). While there was not a statistically significance difference in reproducibility between the OCT models, image quality analysis revealed superior SNR and CNR values for the New OCT in the left eye only, with significant improvements noted (CNR: p = 0.0040 at Visit 2; SNR: p = 0.0383 at Visit 1). Bland-Altman analysis confirmed strong agreement, with minimal mean differences and narrower limits of agreement for the New OCT. Heatmap analysis indicated greater inter-patient variability in the nasal retinal regions, while intra-patient variability was consistently low (<1%) across both devices. PCA and MDS plots affirmed the reproducibility of measurements. Conclusions: Both SD-OCT models provide reliable and consistent TRT measurements, with the New OCT offering marginally enhanced image quality. However, the reproducibility of the New OCT does not significantly outperform the Old OCT, supporting the use of both devices for accurate TRT assessment in clinical settings. Further studies may be required to evaluate these findings in pathological conditions. |
| format | Article |
| id | doaj-art-4aa71037c5d444c2b7e9c3d574717b1e |
| institution | Kabale University |
| issn | 2950-2535 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | AJO International |
| spelling | doaj-art-4aa71037c5d444c2b7e9c3d574717b1e2025-01-25T04:11:31ZengElsevierAJO International2950-25352025-04-0121100099Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machinesKyoung A Viola Lee0Corey Tesdahl1Keith Zimmerman2Kimberly Jun3Sabrina Khalil4Alexander Shahin5Abdullah Abou-Samra6Ramesh Ayyala7Radouil Tzekov8University of South Florida Morsani College of Medicine, 560 Channelside Dr, Tampa FL 33602, USAUniversity of South Florida Morsani College of Medicine, 560 Channelside Dr, Tampa FL 33602, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USAUniversity of South Florida Department of Ophthalmology, 13330 USF Laurel Dr, 4th Floor, Tampa, FL, 33612, USA; Corresponding author.Purpose: Evaluate the integrity, reproducibility, and image quality of total retinal thickness (TRT) measurements between two generations of Spectralis Spectral Domain Optical Coherence Tomography (SD-OCT) instruments (Old OCT: 2011, New OCT: 2017). Design: Prospective cohort study evaluating TRT measurements across two visits. Subjects and participants: Fourteen healthy individuals (28 eyes, age range: 22-54 years) underwent TRT measurements using both Old and New OCT models, with each eye receiving three consecutive scans per visit. Methods and testing: TRT measurements were performed using the Posterior Pole Algorithm (PPA) and Early Treatment Diabetic Retinopathy Study (ETDRS) grid protocols. Reproducibility was evaluated using Average Pairwise Pearson Correlation (APPC), while image quality was measured by Signal-to-Noise Ratio (SNR) and Contrast-to-Noise Ratio (CNR). Agreement between the devices was analyzed through Bland-Altman plots, and spatial variability was visualized using heatmaps. The dimensionality reduction techniques Principal Component Analysis (PCA) and Multidimensional Scaling (MDS) were employed to explore data patterns. Main outcome measures: Reproducibility of TRT measurements, image quality, and the degree of agreement between the two OCT models. Results: Both the Old and New OCT models demonstrated high reproducibility (APPC: 0.995-0.998). While there was not a statistically significance difference in reproducibility between the OCT models, image quality analysis revealed superior SNR and CNR values for the New OCT in the left eye only, with significant improvements noted (CNR: p = 0.0040 at Visit 2; SNR: p = 0.0383 at Visit 1). Bland-Altman analysis confirmed strong agreement, with minimal mean differences and narrower limits of agreement for the New OCT. Heatmap analysis indicated greater inter-patient variability in the nasal retinal regions, while intra-patient variability was consistently low (<1%) across both devices. PCA and MDS plots affirmed the reproducibility of measurements. Conclusions: Both SD-OCT models provide reliable and consistent TRT measurements, with the New OCT offering marginally enhanced image quality. However, the reproducibility of the New OCT does not significantly outperform the Old OCT, supporting the use of both devices for accurate TRT assessment in clinical settings. Further studies may be required to evaluate these findings in pathological conditions.http://www.sciencedirect.com/science/article/pii/S2950253525000024Spectral-domain optical coherence tomography (SD-OCT)Total retinal thicknessBland-Altman analysisOptical imaging |
| spellingShingle | Kyoung A Viola Lee Corey Tesdahl Keith Zimmerman Kimberly Jun Sabrina Khalil Alexander Shahin Abdullah Abou-Samra Ramesh Ayyala Radouil Tzekov Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines AJO International Spectral-domain optical coherence tomography (SD-OCT) Total retinal thickness Bland-Altman analysis Optical imaging |
| title | Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines |
| title_full | Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines |
| title_fullStr | Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines |
| title_full_unstemmed | Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines |
| title_short | Computational analysis of reproducibility and image quality of 2011 and 2017 models of Heidelberg Spectralis SD-OCT machines |
| title_sort | computational analysis of reproducibility and image quality of 2011 and 2017 models of heidelberg spectralis sd oct machines |
| topic | Spectral-domain optical coherence tomography (SD-OCT) Total retinal thickness Bland-Altman analysis Optical imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2950253525000024 |
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