Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC
The validation of underexplored traditional plant remedies represents a reservoir of novel leads for drug discovery. In line with this, in vitro total phenolics and flavonoids content, multimode antioxidants, antimicrobial, cytotoxicity, and protein kinase inhibition assays were conducted on C. ambr...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/6486717 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553603398631424 |
|---|---|
| author | Saira Bano Muhammad Waleed Baig Mohammad K. Okla Syeda Saniya Zahra Nosheen Akhtar Wahidah H. Al-Qahtani Hamada AbdElgawad Ihsan-Ul Haq |
| author_facet | Saira Bano Muhammad Waleed Baig Mohammad K. Okla Syeda Saniya Zahra Nosheen Akhtar Wahidah H. Al-Qahtani Hamada AbdElgawad Ihsan-Ul Haq |
| author_sort | Saira Bano |
| collection | DOAJ |
| description | The validation of underexplored traditional plant remedies represents a reservoir of novel leads for drug discovery. In line with this, in vitro total phenolics and flavonoids content, multimode antioxidants, antimicrobial, cytotoxicity, and protein kinase inhibition assays were conducted on C. ambrosioides seed extracts in addition to RP-HPLC. Methanol extract exhibited highest total phenolics (64.6±0.6 μg gallic acid equivalent/mg) and flavonoids (50.9±0.5 μg quercetin equivalent/mg) content. RP-HPLC quantified rutin (1.98 μg/mg) in methanol extract whereas quercetin (0.322 μg/mg) and kaempferol (2.86 μg/mg) in methanol-distilled water extract. Methanol extract exhibited highest ascorbic acid equivalent (AAE) free radical (DPPH) scavenging (IC50 of 110.7±5 μg/ml), total antioxidant capacity (110.6±2.2 μg AAE/mg), and total reducing power (94.30±0.46 μg AAE/mg). Highest antibacterial activity against K. pneumonia (14±1.61 mm ZOI) and antifungal activity against F. solani (17±1.38 mm ZOI) were shown by n-hexane and chloroform extracts, respectively. Ethyl acetate extract exhibited highest brine shrimps cytotoxicity (LC50 of 125 μg/ml). A noteworthy protein kinase inhibitory potential was shown by ethanol extract with a 20±1.27 mm bald zone. Therapeutic potential of medicinal plants can be completely explored by using multiple solvent system. This study makes C. ambrosioides, a resourceful prospect for the bioactivity-guided isolation of lead compounds. |
| format | Article |
| id | doaj-art-4a9e8b85b4184d769cc2848acee02819 |
| institution | Kabale University |
| issn | 2090-9071 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-4a9e8b85b4184d769cc2848acee028192025-02-03T05:53:39ZengWileyJournal of Chemistry2090-90712022-01-01202210.1155/2022/6486717Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLCSaira Bano0Muhammad Waleed Baig1Mohammad K. Okla2Syeda Saniya Zahra3Nosheen Akhtar4Wahidah H. Al-Qahtani5Hamada AbdElgawad6Ihsan-Ul Haq7Department of PharmacyDepartment of PharmacyBotany and Microbiology DepartmentDepartment of PharmacyDepartment of Biological SciencesDepartment of Food Sciences & NutritionIntegrated Molecular Plant Physiology Research (IMPRES)Department of PharmacyThe validation of underexplored traditional plant remedies represents a reservoir of novel leads for drug discovery. In line with this, in vitro total phenolics and flavonoids content, multimode antioxidants, antimicrobial, cytotoxicity, and protein kinase inhibition assays were conducted on C. ambrosioides seed extracts in addition to RP-HPLC. Methanol extract exhibited highest total phenolics (64.6±0.6 μg gallic acid equivalent/mg) and flavonoids (50.9±0.5 μg quercetin equivalent/mg) content. RP-HPLC quantified rutin (1.98 μg/mg) in methanol extract whereas quercetin (0.322 μg/mg) and kaempferol (2.86 μg/mg) in methanol-distilled water extract. Methanol extract exhibited highest ascorbic acid equivalent (AAE) free radical (DPPH) scavenging (IC50 of 110.7±5 μg/ml), total antioxidant capacity (110.6±2.2 μg AAE/mg), and total reducing power (94.30±0.46 μg AAE/mg). Highest antibacterial activity against K. pneumonia (14±1.61 mm ZOI) and antifungal activity against F. solani (17±1.38 mm ZOI) were shown by n-hexane and chloroform extracts, respectively. Ethyl acetate extract exhibited highest brine shrimps cytotoxicity (LC50 of 125 μg/ml). A noteworthy protein kinase inhibitory potential was shown by ethanol extract with a 20±1.27 mm bald zone. Therapeutic potential of medicinal plants can be completely explored by using multiple solvent system. This study makes C. ambrosioides, a resourceful prospect for the bioactivity-guided isolation of lead compounds.http://dx.doi.org/10.1155/2022/6486717 |
| spellingShingle | Saira Bano Muhammad Waleed Baig Mohammad K. Okla Syeda Saniya Zahra Nosheen Akhtar Wahidah H. Al-Qahtani Hamada AbdElgawad Ihsan-Ul Haq Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC Journal of Chemistry |
| title | Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC |
| title_full | Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC |
| title_fullStr | Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC |
| title_full_unstemmed | Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC |
| title_short | Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC |
| title_sort | antioxidant antimicrobial and protein kinase inhibition profiling of c ambrosioides seed extracts along with rp hplc |
| url | http://dx.doi.org/10.1155/2022/6486717 |
| work_keys_str_mv | AT sairabano antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT muhammadwaleedbaig antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT mohammadkokla antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT syedasaniyazahra antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT nosheenakhtar antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT wahidahhalqahtani antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT hamadaabdelgawad antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc AT ihsanulhaq antioxidantantimicrobialandproteinkinaseinhibitionprofilingofcambrosioidesseedextractsalongwithrphplc |