Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro
In the present study, an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase from a marine bacterium. The prebiotic activity of SPO-1 on the growth of Lactobacillus paracasei and Bifidobacterium animalis, and its effect on human gut microbiota were examined in vitro. The molec...
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Tsinghua University Press
2022-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000781 |
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| author | Bingna Cai Xiangxi Yi Qian Han Jianyu Pan Hua Chen Huili Sun Peng Wan |
| author_facet | Bingna Cai Xiangxi Yi Qian Han Jianyu Pan Hua Chen Huili Sun Peng Wan |
| author_sort | Bingna Cai |
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| description | In the present study, an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase from a marine bacterium. The prebiotic activity of SPO-1 on the growth of Lactobacillus paracasei and Bifidobacterium animalis, and its effect on human gut microbiota were examined in vitro. The molecular weight of the tetrasaccharide SPO-1 was 650.2 Da, and it was mainly composed of glucose with α-type glycosidic linkages. The prebiotic activity score of SPO-1 was the highest for the growth of probiotic strains L. paracasei and B. animalis. Furthermore, as fermentation proceeded, SPO-1 was gradually degraded and utilized by intestinal bacteria. The results showed that after treatment with SPO-1, carbohydrate consumption and short-chain fatty acids levels were increased, especially those of i-butyric and i-valeric acids. Moreover, SPO-1 significantly promoted the abundance, diversity and composition of gut microbiota, especially stimulating the growth of Bacteroides, Escherichia-Shigella and Megamonas. In addition, the change in intestinal microbiota function predicted by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) after treatment with SPO-1 is mainly related to the terms “carbohydrate metabolism” and “amino acid metabolism”. These results suggest that SPO-1 is a potential oligosaccharide in regulation of intestinal microbiota. |
| format | Article |
| id | doaj-art-4a8feef14bb34e3f98c4eb72446526ad |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-4a8feef14bb34e3f98c4eb72446526ad2025-02-03T10:34:58ZengTsinghua University PressFood Science and Human Wellness2213-45302022-01-01111109118Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitroBingna Cai0Xiangxi Yi1Qian Han2Jianyu Pan3Hua Chen4Huili Sun5Peng Wan6Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458 Guangdong, ChinaSchool of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530200 Guangxi, ChinaSchool of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530200 Guangxi, ChinaKey Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, China; Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, 510000 Guangdong, ChinaKey Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458 Guangdong, ChinaKey Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, ChinaKey Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, China; Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, 510000 Guangdong, China; Corresponding author at: Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, China.In the present study, an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase from a marine bacterium. The prebiotic activity of SPO-1 on the growth of Lactobacillus paracasei and Bifidobacterium animalis, and its effect on human gut microbiota were examined in vitro. The molecular weight of the tetrasaccharide SPO-1 was 650.2 Da, and it was mainly composed of glucose with α-type glycosidic linkages. The prebiotic activity score of SPO-1 was the highest for the growth of probiotic strains L. paracasei and B. animalis. Furthermore, as fermentation proceeded, SPO-1 was gradually degraded and utilized by intestinal bacteria. The results showed that after treatment with SPO-1, carbohydrate consumption and short-chain fatty acids levels were increased, especially those of i-butyric and i-valeric acids. Moreover, SPO-1 significantly promoted the abundance, diversity and composition of gut microbiota, especially stimulating the growth of Bacteroides, Escherichia-Shigella and Megamonas. In addition, the change in intestinal microbiota function predicted by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) after treatment with SPO-1 is mainly related to the terms “carbohydrate metabolism” and “amino acid metabolism”. These results suggest that SPO-1 is a potential oligosaccharide in regulation of intestinal microbiota.http://www.sciencedirect.com/science/article/pii/S2213453021000781OligosaccharideSpirulina platensisGut microbiotaIn vitro fermentation |
| spellingShingle | Bingna Cai Xiangxi Yi Qian Han Jianyu Pan Hua Chen Huili Sun Peng Wan Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro Food Science and Human Wellness Oligosaccharide Spirulina platensis Gut microbiota In vitro fermentation |
| title | Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro |
| title_full | Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro |
| title_fullStr | Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro |
| title_full_unstemmed | Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro |
| title_short | Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro |
| title_sort | structural characterization of oligosaccharide from spirulina platensis and its effect on the faecal microbiota in vitro |
| topic | Oligosaccharide Spirulina platensis Gut microbiota In vitro fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000781 |
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