Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque
Abstract Atherosclerosis (AS) is the principal pathological cause of atherosclerotic cardiovascular diseases. Chronic endoplasmic reticulum stress (ERS) has been implicated in AS aetiopathogenesis, but the underlying molecular interactions remain unclear. This study aims to identify the molecular me...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-83925-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594756844126208 |
|---|---|
| author | Ning Zhao Dan Liu Haixu Song Xiaolin Zhang Chenghui Yan Yaling Han |
| author_facet | Ning Zhao Dan Liu Haixu Song Xiaolin Zhang Chenghui Yan Yaling Han |
| author_sort | Ning Zhao |
| collection | DOAJ |
| description | Abstract Atherosclerosis (AS) is the principal pathological cause of atherosclerotic cardiovascular diseases. Chronic endoplasmic reticulum stress (ERS) has been implicated in AS aetiopathogenesis, but the underlying molecular interactions remain unclear. This study aims to identify the molecular mechanisms of ERS in AS pathogenesis to inform innovative diagnostic approaches and therapeutic targets for managing AS. GSE28829 and GSE43292—human early and advanced carotid atherosclerotic tissue samples—were obtained from the Gene Expression Omnibus database. Endoplasmic reticulum stress-related genes (ERSRGs) were obtained from GeneCards. Differential gene expression and weighted gene co-expression network analyses were conducted to identify genes associated with atherosclerosis, and intersection with ER-related genes revealed three ERSRGs (i.e. CTSB, LYN, and CYBB) associated with advanced atherosclerotic plaque. These three ERSRGs exhibited associations with various immune cells. Additionally, the three ERSRGs were upregulated in human atherosclerotic tissues, mouse models of progressive atherosclerotic lesions, and in vitro macrophage models. In conclusion, this study identified CTSB, LYN, and CYBB as potentially critical ERSRGs associated with advanced atherosclerotic plaque, demonstrating their good diagnostic utility and offering novel insights into the potential pathobiology of AS progression, paving the way for exploring innovative therapeutic targets. |
| format | Article |
| id | doaj-art-4a7e4df444e34b68a5f01c1e3e7fcefb |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4a7e4df444e34b68a5f01c1e3e7fcefb2025-01-19T12:20:56ZengNature PortfolioScientific Reports2045-23222025-01-0115112310.1038/s41598-024-83925-zIdentification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaqueNing Zhao0Dan Liu1Haixu Song2Xiaolin Zhang3Chenghui Yan4Yaling Han5Department of Cardiology, Second Norman Bethune Hospital of Jilin UniversityState Key Laboratory of Frigid Zone Cardiovascular Disease, Cardiovascular Research Institute, Department of Cardiology, General Hospital of Northern Theater CommandState Key Laboratory of Frigid Zone Cardiovascular Disease, Cardiovascular Research Institute, Department of Cardiology, General Hospital of Northern Theater CommandState Key Laboratory of Frigid Zone Cardiovascular Disease, Cardiovascular Research Institute, Department of Cardiology, General Hospital of Northern Theater CommandState Key Laboratory of Frigid Zone Cardiovascular Disease, Cardiovascular Research Institute, Department of Cardiology, General Hospital of Northern Theater CommandDepartment of Cardiology, Second Norman Bethune Hospital of Jilin UniversityAbstract Atherosclerosis (AS) is the principal pathological cause of atherosclerotic cardiovascular diseases. Chronic endoplasmic reticulum stress (ERS) has been implicated in AS aetiopathogenesis, but the underlying molecular interactions remain unclear. This study aims to identify the molecular mechanisms of ERS in AS pathogenesis to inform innovative diagnostic approaches and therapeutic targets for managing AS. GSE28829 and GSE43292—human early and advanced carotid atherosclerotic tissue samples—were obtained from the Gene Expression Omnibus database. Endoplasmic reticulum stress-related genes (ERSRGs) were obtained from GeneCards. Differential gene expression and weighted gene co-expression network analyses were conducted to identify genes associated with atherosclerosis, and intersection with ER-related genes revealed three ERSRGs (i.e. CTSB, LYN, and CYBB) associated with advanced atherosclerotic plaque. These three ERSRGs exhibited associations with various immune cells. Additionally, the three ERSRGs were upregulated in human atherosclerotic tissues, mouse models of progressive atherosclerotic lesions, and in vitro macrophage models. In conclusion, this study identified CTSB, LYN, and CYBB as potentially critical ERSRGs associated with advanced atherosclerotic plaque, demonstrating their good diagnostic utility and offering novel insights into the potential pathobiology of AS progression, paving the way for exploring innovative therapeutic targets.https://doi.org/10.1038/s41598-024-83925-zAtherosclerosisEndoplasmic reticulum stressImmune infiltrationBioinformatics analysis |
| spellingShingle | Ning Zhao Dan Liu Haixu Song Xiaolin Zhang Chenghui Yan Yaling Han Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque Scientific Reports Atherosclerosis Endoplasmic reticulum stress Immune infiltration Bioinformatics analysis |
| title | Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque |
| title_full | Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque |
| title_fullStr | Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque |
| title_full_unstemmed | Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque |
| title_short | Identification of critical endoplasmic reticulum stress-related genes in advanced atherosclerotic plaque |
| title_sort | identification of critical endoplasmic reticulum stress related genes in advanced atherosclerotic plaque |
| topic | Atherosclerosis Endoplasmic reticulum stress Immune infiltration Bioinformatics analysis |
| url | https://doi.org/10.1038/s41598-024-83925-z |
| work_keys_str_mv | AT ningzhao identificationofcriticalendoplasmicreticulumstressrelatedgenesinadvancedatheroscleroticplaque AT danliu identificationofcriticalendoplasmicreticulumstressrelatedgenesinadvancedatheroscleroticplaque AT haixusong identificationofcriticalendoplasmicreticulumstressrelatedgenesinadvancedatheroscleroticplaque AT xiaolinzhang identificationofcriticalendoplasmicreticulumstressrelatedgenesinadvancedatheroscleroticplaque AT chenghuiyan identificationofcriticalendoplasmicreticulumstressrelatedgenesinadvancedatheroscleroticplaque AT yalinghan identificationofcriticalendoplasmicreticulumstressrelatedgenesinadvancedatheroscleroticplaque |