Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice
The aim of this research is to investigate the therapeutic effect of GGQL decoction on cardiac dysfunction and elucidate the pharmacological mechanisms. db/db mice were divided into DB group or GGQL group, and WT mice were used as control. All mice were accessed by echocardiography. And the total RN...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/7421761 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548050368724992 |
|---|---|
| author | Jing Han Zhenglin Wang Wei Xing Yueying Yuan Yi Zhang Tiantian Lv Hongliang Wang Yonggang Liu Yan Wu |
| author_facet | Jing Han Zhenglin Wang Wei Xing Yueying Yuan Yi Zhang Tiantian Lv Hongliang Wang Yonggang Liu Yan Wu |
| author_sort | Jing Han |
| collection | DOAJ |
| description | The aim of this research is to investigate the therapeutic effect of GGQL decoction on cardiac dysfunction and elucidate the pharmacological mechanisms. db/db mice were divided into DB group or GGQL group, and WT mice were used as control. All mice were accessed by echocardiography. And the total RNA of LV tissue samples was sequenced, then differential expression genes were analyzed. The RNA-seq results were validated by the results of RT-qPCR of 4 genes identified as differentially expressed. The content of pyruvate and ceramide in myocardial tissue was also measured. The results showed that GGQL decoction could significantly improve the diastolic dysfunction, increase the content of pyruvate, and had the trend to reduce the ceramide content. The results of RNA-seq showed that 2958 genes were differentially expressed when comparing the DB group with the WT group. Among them, compared with the DB group, 26 genes were differentially regulated in the GGQL group. The expression results of 4 genes were consistent with the RNA-seq results. Our study reveals that GGQL decoction has a therapeutic effect on diastolic dysfunction of the left ventricular and the effect may be related to its role in promoting myocardial glycolysis and decreasing the content of ceramide. |
| format | Article |
| id | doaj-art-4a512d1a2da54ea39256acbb67cb6c53 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4a512d1a2da54ea39256acbb67cb6c532025-02-03T06:42:16ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/74217617421761Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic MiceJing Han0Zhenglin Wang1Wei Xing2Yueying Yuan3Yi Zhang4Tiantian Lv5Hongliang Wang6Yonggang Liu7Yan Wu8Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and Formulas), Key Laboratory of Beijing (Syndromes and Formulas), Beijing University of Chinese Medicine, Beijing 100029, ChinaCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and Formulas), Key Laboratory of Beijing (Syndromes and Formulas), Beijing University of Chinese Medicine, Beijing 100029, ChinaInstitute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaModern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and Formulas), Key Laboratory of Beijing (Syndromes and Formulas), Beijing University of Chinese Medicine, Beijing 100029, ChinaCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and Formulas), Key Laboratory of Beijing (Syndromes and Formulas), Beijing University of Chinese Medicine, Beijing 100029, ChinaCollege of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaInstitute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaThe aim of this research is to investigate the therapeutic effect of GGQL decoction on cardiac dysfunction and elucidate the pharmacological mechanisms. db/db mice were divided into DB group or GGQL group, and WT mice were used as control. All mice were accessed by echocardiography. And the total RNA of LV tissue samples was sequenced, then differential expression genes were analyzed. The RNA-seq results were validated by the results of RT-qPCR of 4 genes identified as differentially expressed. The content of pyruvate and ceramide in myocardial tissue was also measured. The results showed that GGQL decoction could significantly improve the diastolic dysfunction, increase the content of pyruvate, and had the trend to reduce the ceramide content. The results of RNA-seq showed that 2958 genes were differentially expressed when comparing the DB group with the WT group. Among them, compared with the DB group, 26 genes were differentially regulated in the GGQL group. The expression results of 4 genes were consistent with the RNA-seq results. Our study reveals that GGQL decoction has a therapeutic effect on diastolic dysfunction of the left ventricular and the effect may be related to its role in promoting myocardial glycolysis and decreasing the content of ceramide.http://dx.doi.org/10.1155/2017/7421761 |
| spellingShingle | Jing Han Zhenglin Wang Wei Xing Yueying Yuan Yi Zhang Tiantian Lv Hongliang Wang Yonggang Liu Yan Wu Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice International Journal of Genomics |
| title | Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice |
| title_full | Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice |
| title_fullStr | Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice |
| title_full_unstemmed | Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice |
| title_short | Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice |
| title_sort | effect of gegen qinlian decoction on cardiac gene expression in diabetic mice |
| url | http://dx.doi.org/10.1155/2017/7421761 |
| work_keys_str_mv | AT jinghan effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT zhenglinwang effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT weixing effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT yueyingyuan effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT yizhang effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT tiantianlv effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT hongliangwang effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT yonggangliu effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice AT yanwu effectofgegenqinliandecoctiononcardiacgeneexpressionindiabeticmice |