Protocol for generating protein profiles and distance-based network analysis of murine tissue slices
Summary: We introduce a protocol for spatial proteomics using thin cryotome sections of mouse skeletal muscle tissue. We describe steps for preparing muscle sections and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses to generate spatial protein profiles along the longitudinal ske...
Saved in:
Main Authors: | Luisa Schmidt, Philipp Antczak, Marcus Krüger |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007433 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Identification of meibomian gland testosterone metabolites produced by tissue-intrinsic intracrine deactivation activity
by: Khanh Tien Nguyen Pham, et al.
Published: (2025-02-01) -
Perturbation of EPHA2 and EFNA1 trans binding amplifies inflammatory response in airway epithelial cells
by: Ryosuke Fukuda, et al.
Published: (2025-02-01) -
Protocol for assessing the clogging of the mitochondrial translocase of the outer membrane by precursor proteins in human cells
by: John Kim, et al.
Published: (2025-03-01) -
Protocol for predicting host-microbe interactions and their downstream effect on host cells using MicrobioLink
by: Lejla Gul, et al.
Published: (2025-03-01) -
UFMylation maintains YAP stability to promote vascular endothelial cell senescence
by: Yanan Liu, et al.
Published: (2025-02-01)