Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures
Abstract This study aimed to compare the failure rates of two different sizes of plates and screws to stabilize critical-sized (7 mm) femoral defects in male Sprague‒Dawley rats (aged 10 weeks). Femoral defects were stabilized with either a 4-hole plate (length 29 mm, thickness 1 mm, 10 rats, Group...
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Nature Portfolio
2025-01-01
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| author | Marc Saab Anne-Sophie Drucbert Nicolas Blanchemain Feng Chai |
| author_facet | Marc Saab Anne-Sophie Drucbert Nicolas Blanchemain Feng Chai |
| author_sort | Marc Saab |
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| description | Abstract This study aimed to compare the failure rates of two different sizes of plates and screws to stabilize critical-sized (7 mm) femoral defects in male Sprague‒Dawley rats (aged 10 weeks). Femoral defects were stabilized with either a 4-hole plate (length 29 mm, thickness 1 mm, 10 rats, Group 1) and 4 cortical screws (diameter 2 mm) or with a 6-hole plate (length 30 mm, thickness 0.6 mm, 9 rats, Group 2) and 4 cortical screws (diameter 1.5 mm). A polymethylmethacrylate spacer was inserted into the defects to reproduce the first stage of the induced membrane technique. Radiographic evaluations, macroscopic and histologic assessments of the induced membranes were conducted at 1 week and 4 weeks. No implant failure occurred in Group 1 whereas in Group 2, 4/9 (44.4%) implant failures occurred during the follow-up (p = 0.03). On histomorphometry, cell density was higher in Group 1 (4996 ± 716 cells / mm²) than in Group 2 (3500 ± 728 cells/mm²) (p = 0.0195) but the membrane thickness in Group 1 (735 ± 44 μm) was non-significantly lower than in Group 2 (979 ± 165 μm) (p = 0.4). This study suggests that, in rat models of critical-sized femoral defects (7 mm) to study the induced membrane technique, fixation plates with a thickness of 1 mm and four screws (2 mm in diameter) provide stable fixation without implant failure. In contrast, thinner plates (< 1 mm) combined with screws of smaller diameter (1.5 mm) result in a high rate of implant failure. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-4a4df68871ce4d01b2a4d6956c4c26df2025-01-26T12:32:05ZengNature PortfolioScientific Reports2045-23222025-01-0115111010.1038/s41598-025-85607-wComparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failuresMarc Saab0Anne-Sophie Drucbert1Nicolas Blanchemain2Feng Chai3Service d’Orthopédie 1 et Traumatologie, Hôpital Roger SalengroU1008 – Advanced Drug Delivery Systems and Biomaterials, Univ. Lille, INSERM, CHU LilleU1008 – Advanced Drug Delivery Systems and Biomaterials, Univ. Lille, INSERM, CHU LilleU1008 – Advanced Drug Delivery Systems and Biomaterials, Univ. Lille, INSERM, CHU LilleAbstract This study aimed to compare the failure rates of two different sizes of plates and screws to stabilize critical-sized (7 mm) femoral defects in male Sprague‒Dawley rats (aged 10 weeks). Femoral defects were stabilized with either a 4-hole plate (length 29 mm, thickness 1 mm, 10 rats, Group 1) and 4 cortical screws (diameter 2 mm) or with a 6-hole plate (length 30 mm, thickness 0.6 mm, 9 rats, Group 2) and 4 cortical screws (diameter 1.5 mm). A polymethylmethacrylate spacer was inserted into the defects to reproduce the first stage of the induced membrane technique. Radiographic evaluations, macroscopic and histologic assessments of the induced membranes were conducted at 1 week and 4 weeks. No implant failure occurred in Group 1 whereas in Group 2, 4/9 (44.4%) implant failures occurred during the follow-up (p = 0.03). On histomorphometry, cell density was higher in Group 1 (4996 ± 716 cells / mm²) than in Group 2 (3500 ± 728 cells/mm²) (p = 0.0195) but the membrane thickness in Group 1 (735 ± 44 μm) was non-significantly lower than in Group 2 (979 ± 165 μm) (p = 0.4). This study suggests that, in rat models of critical-sized femoral defects (7 mm) to study the induced membrane technique, fixation plates with a thickness of 1 mm and four screws (2 mm in diameter) provide stable fixation without implant failure. In contrast, thinner plates (< 1 mm) combined with screws of smaller diameter (1.5 mm) result in a high rate of implant failure.https://doi.org/10.1038/s41598-025-85607-wRat modelFemoral defectsInduced membrane techniqueMasquelet technique |
| spellingShingle | Marc Saab Anne-Sophie Drucbert Nicolas Blanchemain Feng Chai Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures Scientific Reports Rat model Femoral defects Induced membrane technique Masquelet technique |
| title | Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures |
| title_full | Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures |
| title_fullStr | Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures |
| title_full_unstemmed | Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures |
| title_short | Comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures |
| title_sort | comparison of two plates and screw osteosynthesis configurations in a rat model of critical sized femoral defects to reduce implant related failures |
| topic | Rat model Femoral defects Induced membrane technique Masquelet technique |
| url | https://doi.org/10.1038/s41598-025-85607-w |
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