Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i>
<i>Camellia oleifera</i> is a unique woody edible oil tree species in China, and the ovule development affects the yield of seeds. This study selected three different types of <i>C. oleifera</i> clones and used LC-MS, RNA-seq, and other techniques to compare the endogenous ho...
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MDPI AG
2025-02-01
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| Series: | Plants |
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| author | Yayan Zhu Jiajuan Xu Gang Wang Feng Xiao Minggang Zhang Qinmeng Zeng Jie Xu |
| author_facet | Yayan Zhu Jiajuan Xu Gang Wang Feng Xiao Minggang Zhang Qinmeng Zeng Jie Xu |
| author_sort | Yayan Zhu |
| collection | DOAJ |
| description | <i>Camellia oleifera</i> is a unique woody edible oil tree species in China, and the ovule development affects the yield of seeds. This study selected three different types of <i>C. oleifera</i> clones and used LC-MS, RNA-seq, and other techniques to compare the endogenous hormone contents, gene expression levels, and metabolite changes between normal and aborted ovules. The results showed that high levels of ABA, JA, and SA may lead to the phenotype of ovule abortion. A total of 270 differential metabolites were identified in the metabolome, with L-methionine, citrulline, L-tryptophan, L-phenylalanine, and indolepyruvate being downregulated to varying degrees in the aborted ovules. Genes involved in plant hormone synthesis and response, such as <i>GH3.1</i>, <i>IAA14</i>, <i>PIN1</i>, <i>AUX22</i>, <i>ARF1_2</i>, <i>BZR1_2</i>, <i>GA2ox</i>, <i>ERFC3</i>, <i>ABF2</i>, and <i>PYL8</i>, responded to ovule development. This study elucidates the physiological, metabolic, and transcriptional responses to ovule abortion, providing a theoretical basis for understanding ovule development and yield regulation in <i>C. oleifera</i>. |
| format | Article |
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| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-4a2aeb36e4fb430aa9a3b64e12c0b5ab2025-08-20T02:44:56ZengMDPI AGPlants2223-77472025-02-0114461310.3390/plants14040613Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i>Yayan Zhu0Jiajuan Xu1Gang Wang2Feng Xiao3Minggang Zhang4Qinmeng Zeng5Jie Xu6Guizhou Academy of Forestry, Guiyang 550005, ChinaGuizhou Academy of Forestry, Guiyang 550005, ChinaGuizhou Academy of Forestry, Guiyang 550005, ChinaInstitute for Forest Resources and Environment of Guizhou, Guizhou University, Guiyang 550025, ChinaGuizhou Academy of Forestry, Guiyang 550005, ChinaGuizhou Academy of Forestry, Guiyang 550005, ChinaGuizhou Academy of Forestry, Guiyang 550005, China<i>Camellia oleifera</i> is a unique woody edible oil tree species in China, and the ovule development affects the yield of seeds. This study selected three different types of <i>C. oleifera</i> clones and used LC-MS, RNA-seq, and other techniques to compare the endogenous hormone contents, gene expression levels, and metabolite changes between normal and aborted ovules. The results showed that high levels of ABA, JA, and SA may lead to the phenotype of ovule abortion. A total of 270 differential metabolites were identified in the metabolome, with L-methionine, citrulline, L-tryptophan, L-phenylalanine, and indolepyruvate being downregulated to varying degrees in the aborted ovules. Genes involved in plant hormone synthesis and response, such as <i>GH3.1</i>, <i>IAA14</i>, <i>PIN1</i>, <i>AUX22</i>, <i>ARF1_2</i>, <i>BZR1_2</i>, <i>GA2ox</i>, <i>ERFC3</i>, <i>ABF2</i>, and <i>PYL8</i>, responded to ovule development. This study elucidates the physiological, metabolic, and transcriptional responses to ovule abortion, providing a theoretical basis for understanding ovule development and yield regulation in <i>C. oleifera</i>.https://www.mdpi.com/2223-7747/14/4/613abortion<i>Camellia oleifera</i>LC-MSovuleabortiontranscriptomics |
| spellingShingle | Yayan Zhu Jiajuan Xu Gang Wang Feng Xiao Minggang Zhang Qinmeng Zeng Jie Xu Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i> Plants abortion <i>Camellia oleifera</i> LC-MS ovule abortion transcriptomics |
| title | Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i> |
| title_full | Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i> |
| title_fullStr | Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i> |
| title_full_unstemmed | Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i> |
| title_short | Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i> |
| title_sort | integrated metabolome and transcriptome analyses provides insights into ovule abortion in i camellia oleifera i |
| topic | abortion <i>Camellia oleifera</i> LC-MS ovule abortion transcriptomics |
| url | https://www.mdpi.com/2223-7747/14/4/613 |
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