Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation
Introduction Mesenchymal stem cells (MSCs) have promising regenerative properties in tissue repair and anti-inflammatory responses. This study aimed to investigate the effects of MSCs and their combination with micronized purified flavonoid fraction (MPFF) in a croton oil-induced hemorrhoids model o...
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| Language: | English |
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Termedia Publishing House
2024-10-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/Synergistic-therapeutic-approach-for-hemorrhoids-integrating-mesenchymal-stem-cells,183465,0,2.html |
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| author | M. Hidayat Budi Kusumo Adi Prayitno Soetrisno . Abdurahman Laqif |
| author_facet | M. Hidayat Budi Kusumo Adi Prayitno Soetrisno . Abdurahman Laqif |
| author_sort | M. Hidayat Budi Kusumo |
| collection | DOAJ |
| description | Introduction
Mesenchymal stem cells (MSCs) have promising regenerative properties in tissue repair and anti-inflammatory responses. This study aimed to investigate the effects of MSCs and their combination with micronized purified flavonoid fraction (MPFF) in a croton oil-induced hemorrhoids model on tissue edema, inflammation, and underlying molecular mechanisms.
Material and methods
MSCs were isolated and characterized for their adherence, differentiation capacity, and immunophenotyping. Croton oil-induced hemorrhoid mouse models were established to assess tissue edema, inflammation, tumor necrosis factor (TNF-α) expression, transforming growth factor-β (TGF-β) expression, collagen ratio, and MMP-9 activity. The effects of MSCs and their combination with MPFF (diosmin-hesperidin) were evaluated through histological examinations, western blot analysis, and gelatin zymography.
Results
Characterization confirmed the MSCs’ plastic adherence, osteogenic differentiation potential, and immunophenotype (positive for CD90 and CD29, negative for CD45 and CD31). Treatment with MSCs alone or in combination with MPFF significantly reduced tissue edema, inflammation, TNF-α expression, and MMP-9 activity. Additionally, MSCs increased TGF-β expression, and collagen type I/III ratio, and accelerated wound healing by resolving inflammation.
Conclusions
These findings suggest that MSCs play a crucial role in modulating TNF-α, TGF-β, collagen remodeling, and MMP-9 activity, highlighting their promising role in hemorrhoid treatment and wound healing processes. Further research is warranted to fully elucidate the intricate mechanisms and optimize MSC-based therapies for clinical applications in hemorrhoidal disease management. |
| format | Article |
| id | doaj-art-4a286ce0ad5448069a97fc673e4c4f67 |
| institution | Kabale University |
| issn | 1734-1922 1896-9151 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj-art-4a286ce0ad5448069a97fc673e4c4f672025-01-27T10:44:31ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512024-10-012051556156610.5114/aoms/183465183465Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammationM. Hidayat Budi Kusumo0Adi Prayitno1Soetrisno .2Abdurahman Laqif3Doctoral program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, IndonesiaDoctoral program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, IndonesiaDoctoral program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, IndonesiaDoctoral program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, IndonesiaIntroduction Mesenchymal stem cells (MSCs) have promising regenerative properties in tissue repair and anti-inflammatory responses. This study aimed to investigate the effects of MSCs and their combination with micronized purified flavonoid fraction (MPFF) in a croton oil-induced hemorrhoids model on tissue edema, inflammation, and underlying molecular mechanisms. Material and methods MSCs were isolated and characterized for their adherence, differentiation capacity, and immunophenotyping. Croton oil-induced hemorrhoid mouse models were established to assess tissue edema, inflammation, tumor necrosis factor (TNF-α) expression, transforming growth factor-β (TGF-β) expression, collagen ratio, and MMP-9 activity. The effects of MSCs and their combination with MPFF (diosmin-hesperidin) were evaluated through histological examinations, western blot analysis, and gelatin zymography. Results Characterization confirmed the MSCs’ plastic adherence, osteogenic differentiation potential, and immunophenotype (positive for CD90 and CD29, negative for CD45 and CD31). Treatment with MSCs alone or in combination with MPFF significantly reduced tissue edema, inflammation, TNF-α expression, and MMP-9 activity. Additionally, MSCs increased TGF-β expression, and collagen type I/III ratio, and accelerated wound healing by resolving inflammation. Conclusions These findings suggest that MSCs play a crucial role in modulating TNF-α, TGF-β, collagen remodeling, and MMP-9 activity, highlighting their promising role in hemorrhoid treatment and wound healing processes. Further research is warranted to fully elucidate the intricate mechanisms and optimize MSC-based therapies for clinical applications in hemorrhoidal disease management.https://www.archivesofmedicalscience.com/Synergistic-therapeutic-approach-for-hemorrhoids-integrating-mesenchymal-stem-cells,183465,0,2.htmlmesenchymal stem cellscroton oilhemorrhoidinflammationmicronized purified flavonoid fractiondiosmin-hesperidin |
| spellingShingle | M. Hidayat Budi Kusumo Adi Prayitno Soetrisno . Abdurahman Laqif Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation Archives of Medical Science mesenchymal stem cells croton oil hemorrhoid inflammation micronized purified flavonoid fraction diosmin-hesperidin |
| title | Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation |
| title_full | Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation |
| title_fullStr | Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation |
| title_full_unstemmed | Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation |
| title_short | Synergistic therapeutic approach for hemorrhoids: integrating mesenchymal stem cells with diosmin-hesperidin to target tissue edema and inflammation |
| title_sort | synergistic therapeutic approach for hemorrhoids integrating mesenchymal stem cells with diosmin hesperidin to target tissue edema and inflammation |
| topic | mesenchymal stem cells croton oil hemorrhoid inflammation micronized purified flavonoid fraction diosmin-hesperidin |
| url | https://www.archivesofmedicalscience.com/Synergistic-therapeutic-approach-for-hemorrhoids-integrating-mesenchymal-stem-cells,183465,0,2.html |
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