Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk
Casein and α-lactalbumin (α-LA) are the main allergens in cow's milk, which can affect the skin, respiratory system, and gastrointestinal tract, even cause anaphylactic shock. Developing a rapid and sensitive detection method of casein and α-LA is still pursued. Herein, a surface-enhanced Raman...
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002142 |
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| author | Jing Li Jia Xu Yi Pan Yongzhi Zhu Yuanfeng Wang Shouhui Chen Xinlin Wei |
| author_facet | Jing Li Jia Xu Yi Pan Yongzhi Zhu Yuanfeng Wang Shouhui Chen Xinlin Wei |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Casein and α-lactalbumin (α-LA) are the main allergens in cow's milk, which can affect the skin, respiratory system, and gastrointestinal tract, even cause anaphylactic shock. Developing a rapid and sensitive detection method of casein and α-LA is still pursued. Herein, a surface-enhanced Raman scattering based lateral flow assay (SERS-LFA) method for rapid and highly sensitive detection of milk allergens in food was established for effectively preventing allergic symptoms. Gold@silver nanoparticles (Au@Ag NPs) were synthesized as SERS active substrate to prepare the antibody-modified SERS Probe and SERS-LFA strips toward casein and α-LA were assembled according to the sandwich mode. The detection results were calculated according to colorimetric and Raman signal. The introduction of SRES signal significantly increased the sensitivity of detection with the limit of detection (LOD) of 0.19 ng/mL and 1.74 pg/mL, and exhibited an excellent linear relationship within the range of 0.55–791.50 ng/mL and 0.1 pg/mL–100.0 ng/mL for casein and α-LA, respectively. Furthermore, SERS-LFA strips was highly specific with the recovery rates for corresponding 80.36 %–105.12 % and 85.73 %–118.22 % for casein and α-LA, respectively. Therefore, the SERS-LFA could be in great potential to develop a unique allergen detection method. |
| format | Article |
| id | doaj-art-4a19aea1bc644cd2bbc2e63cc5ef2414 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-4a19aea1bc644cd2bbc2e63cc5ef24142025-02-03T10:34:09ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123912919Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milkJing Li0Jia Xu1Yi Pan2Yongzhi Zhu3Yuanfeng Wang4Shouhui Chen5Xinlin Wei6Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaInstitute of Food Engineering, College of Life Science, Shanghai Normal University, Shanghai 200234, ChinaInstitute of Food Engineering, College of Life Science, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding author at: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Casein and α-lactalbumin (α-LA) are the main allergens in cow's milk, which can affect the skin, respiratory system, and gastrointestinal tract, even cause anaphylactic shock. Developing a rapid and sensitive detection method of casein and α-LA is still pursued. Herein, a surface-enhanced Raman scattering based lateral flow assay (SERS-LFA) method for rapid and highly sensitive detection of milk allergens in food was established for effectively preventing allergic symptoms. Gold@silver nanoparticles (Au@Ag NPs) were synthesized as SERS active substrate to prepare the antibody-modified SERS Probe and SERS-LFA strips toward casein and α-LA were assembled according to the sandwich mode. The detection results were calculated according to colorimetric and Raman signal. The introduction of SRES signal significantly increased the sensitivity of detection with the limit of detection (LOD) of 0.19 ng/mL and 1.74 pg/mL, and exhibited an excellent linear relationship within the range of 0.55–791.50 ng/mL and 0.1 pg/mL–100.0 ng/mL for casein and α-LA, respectively. Furthermore, SERS-LFA strips was highly specific with the recovery rates for corresponding 80.36 %–105.12 % and 85.73 %–118.22 % for casein and α-LA, respectively. Therefore, the SERS-LFA could be in great potential to develop a unique allergen detection method.http://www.sciencedirect.com/science/article/pii/S2213453022002142Caseinα-LactalbuminAu@Ag NPsSERS-LFA |
| spellingShingle | Jing Li Jia Xu Yi Pan Yongzhi Zhu Yuanfeng Wang Shouhui Chen Xinlin Wei Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk Food Science and Human Wellness Casein α-Lactalbumin Au@Ag NPs SERS-LFA |
| title | Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk |
| title_full | Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk |
| title_fullStr | Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk |
| title_full_unstemmed | Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk |
| title_short | Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk |
| title_sort | au ag labeled sers lateral flow assay for highly sensitive detection of allergens in milk |
| topic | Casein α-Lactalbumin Au@Ag NPs SERS-LFA |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002142 |
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