Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions
Introduction. Although it is well known that BMP-2 and BMP-7 play significant roles in cartilage metabolism, data about intra-articular expression and localization of these proteins and their receptors in humans are rare. Methods. Biopsies of synovia and debrided cartilage were taken in patients und...
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2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/467892 |
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| author | Hagen Schmal Alexander T. Mehlhorn Ingo H. Pilz David Dovi-Akue Christina Kirchhoff Norbert P. Südkamp Ulrike Gerlach Christian Lohrmann Philipp Niemeyer |
| author_facet | Hagen Schmal Alexander T. Mehlhorn Ingo H. Pilz David Dovi-Akue Christina Kirchhoff Norbert P. Südkamp Ulrike Gerlach Christian Lohrmann Philipp Niemeyer |
| author_sort | Hagen Schmal |
| collection | DOAJ |
| description | Introduction. Although it is well known that BMP-2 and BMP-7 play significant roles in cartilage metabolism, data about intra-articular expression and localization of these proteins and their receptors in humans are rare. Methods. Biopsies of synovia and debrided cartilage were taken in patients undergoing autologous chondrocyte implantation. Expression of BMP-2, BMP-7, and their receptors BMPR-1A, BMPR-1B and BMPR-2 were semiquantitatively evaluated by immunohistological staining. Results. BMP-7 was equally highly expressed in all cartilage and synovial biopsies. Increased levels of BMPR-1A, but not of BMPR-1B, and BMPR-2, were found in all synovial and 47% of all cartilage samples (P=0.002). BMP-2 was positively scored in 47% of all cartilage and 40% of all synovial specimens. Defect size, KOSS, Henderson or Kellgren-Lawrence score did not statistically significant correlate with the expression of the analyzed proteins or Mankin and Pritzker scores. Duration of symptoms and localization of lesions were associated with KOSS (P<0.02), but there was no influence of these parameters on protein expression. Conclusions. BMP-2, BMP-7, and BMPR-1A were expressed in cartilage and synovia of knees with focal cartilage lesions. Although defect localization and duration of symptoms decisively influence KOSS, there was no associated alteration of protein expression observed. |
| format | Article |
| id | doaj-art-4a0b72b247a04ef7953525763d47a329 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4a0b72b247a04ef7953525763d47a3292025-02-03T06:04:47ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/467892467892Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage LesionsHagen Schmal0Alexander T. Mehlhorn1Ingo H. Pilz2David Dovi-Akue3Christina Kirchhoff4Norbert P. Südkamp5Ulrike Gerlach6Christian Lohrmann7Philipp Niemeyer8Department of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyDepartment of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyDepartment of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyDepartment of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyDepartment of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyDepartment of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyInstitute of Pathology, University of Medical Center Freiburg, Breisacher Street 115a, 79106 Freiburg, GermanyDepartment of Radiology, University of Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyDepartment of Orthopaedic Surgery, University Medical Center Freiburg, Hugstetter Street 55, 79106 Freiburg, GermanyIntroduction. Although it is well known that BMP-2 and BMP-7 play significant roles in cartilage metabolism, data about intra-articular expression and localization of these proteins and their receptors in humans are rare. Methods. Biopsies of synovia and debrided cartilage were taken in patients undergoing autologous chondrocyte implantation. Expression of BMP-2, BMP-7, and their receptors BMPR-1A, BMPR-1B and BMPR-2 were semiquantitatively evaluated by immunohistological staining. Results. BMP-7 was equally highly expressed in all cartilage and synovial biopsies. Increased levels of BMPR-1A, but not of BMPR-1B, and BMPR-2, were found in all synovial and 47% of all cartilage samples (P=0.002). BMP-2 was positively scored in 47% of all cartilage and 40% of all synovial specimens. Defect size, KOSS, Henderson or Kellgren-Lawrence score did not statistically significant correlate with the expression of the analyzed proteins or Mankin and Pritzker scores. Duration of symptoms and localization of lesions were associated with KOSS (P<0.02), but there was no influence of these parameters on protein expression. Conclusions. BMP-2, BMP-7, and BMPR-1A were expressed in cartilage and synovia of knees with focal cartilage lesions. Although defect localization and duration of symptoms decisively influence KOSS, there was no associated alteration of protein expression observed.http://dx.doi.org/10.1100/2012/467892 |
| spellingShingle | Hagen Schmal Alexander T. Mehlhorn Ingo H. Pilz David Dovi-Akue Christina Kirchhoff Norbert P. Südkamp Ulrike Gerlach Christian Lohrmann Philipp Niemeyer Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions The Scientific World Journal |
| title | Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions |
| title_full | Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions |
| title_fullStr | Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions |
| title_full_unstemmed | Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions |
| title_short | Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions |
| title_sort | immunohistological localization of bmp 2 bmp 7 and their receptors in knee joints with focal cartilage lesions |
| url | http://dx.doi.org/10.1100/2012/467892 |
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