Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments
The microbial potential of Penicillium has received critical attention. The present research aimed to elucidate the efficacy of crude enzyme secreted from Penicillium oxalicum WX-209 in degrading citrus segments and evaluate the safety of the process. Results showed that citrus segment membranes gra...
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000575 |
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| author | Xiao Hu Yujiao Qian Zhipeng Gao Gaoyang Li Fuhua Fu Jiajing Guo Yang Shan |
| author_facet | Xiao Hu Yujiao Qian Zhipeng Gao Gaoyang Li Fuhua Fu Jiajing Guo Yang Shan |
| author_sort | Xiao Hu |
| collection | DOAJ |
| description | The microbial potential of Penicillium has received critical attention. The present research aimed to elucidate the efficacy of crude enzyme secreted from Penicillium oxalicum WX-209 in degrading citrus segments and evaluate the safety of the process. Results showed that citrus segment membranes gradually dissolved after treatment with the crude enzyme solution, indicating good degradation capability. No significant differences in body weight, food ingestion rate, hematology, blood biochemistry, and weight changes of different organs were found between the enzyme intake and control groups. Serial experiments showed that the crude enzyme had high biological safety. Moreover, the whole genome of P. oxalicum WX-209 was sequenced by PacBio and Illumina platforms. Twenty-five scaffolds were assembled to generate 36 Mbp size of genome sequence comprising 11 369 predicted genes modeled with a GC content of 48.33 %. A total of 592 genes were annotated to encode enzymes related to carbohydrates, and some degradation enzyme genes were identified in strain P. oxalicum WX-209. Data availability: The whole genome sequencing data in this study can be found in GenBank database of NCBI website with the accession number PRJNA 715321. The link: http://www.ncbi.nlm.nih.gov/bioproject/715321. |
| format | Article |
| id | doaj-art-49f3f39d48384a94b92a41c2e1c75c9d |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-49f3f39d48384a94b92a41c2e1c75c9d2025-02-03T02:42:14ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112623692380Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segmentsXiao Hu0Yujiao Qian1Zhipeng Gao2Gaoyang Li3Fuhua Fu4Jiajing Guo5Yang Shan6Hunan Agriculture Product Processing Institute, International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits & Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agriculture Product Processing Institute, International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits & Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Agriculture Product Processing Institute, International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits & Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agriculture Product Processing Institute, International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits & Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agriculture Product Processing Institute, International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits & Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Corresponding authors.Hunan Agriculture Product Processing Institute, International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits & Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Corresponding authors.The microbial potential of Penicillium has received critical attention. The present research aimed to elucidate the efficacy of crude enzyme secreted from Penicillium oxalicum WX-209 in degrading citrus segments and evaluate the safety of the process. Results showed that citrus segment membranes gradually dissolved after treatment with the crude enzyme solution, indicating good degradation capability. No significant differences in body weight, food ingestion rate, hematology, blood biochemistry, and weight changes of different organs were found between the enzyme intake and control groups. Serial experiments showed that the crude enzyme had high biological safety. Moreover, the whole genome of P. oxalicum WX-209 was sequenced by PacBio and Illumina platforms. Twenty-five scaffolds were assembled to generate 36 Mbp size of genome sequence comprising 11 369 predicted genes modeled with a GC content of 48.33 %. A total of 592 genes were annotated to encode enzymes related to carbohydrates, and some degradation enzyme genes were identified in strain P. oxalicum WX-209. Data availability: The whole genome sequencing data in this study can be found in GenBank database of NCBI website with the accession number PRJNA 715321. The link: http://www.ncbi.nlm.nih.gov/bioproject/715321.http://www.sciencedirect.com/science/article/pii/S2213453023000575Penicillium oxalicum WX-209Crude enzymeDegradationSafety evaluationGenome sequencing |
| spellingShingle | Xiao Hu Yujiao Qian Zhipeng Gao Gaoyang Li Fuhua Fu Jiajing Guo Yang Shan Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments Food Science and Human Wellness Penicillium oxalicum WX-209 Crude enzyme Degradation Safety evaluation Genome sequencing |
| title | Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments |
| title_full | Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments |
| title_fullStr | Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments |
| title_full_unstemmed | Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments |
| title_short | Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments |
| title_sort | safety evaluation and whole genome sequencing for revealing the ability of penicillium oxalicum wx 209 to safely and effectively degrade citrus segments |
| topic | Penicillium oxalicum WX-209 Crude enzyme Degradation Safety evaluation Genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000575 |
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