Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation
Exosomes are nanovesicles released by different cell types, such as dendritic cells (DCs), mast cells (MCs), and tumor cells. Exosomes of different origin play a role in antigen presentation and modulation of immune response to infectious disease. In this study, we demonstrate that mast cells and CD...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3623898 |
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| author | Fei Li Yuping Wang Lihui Lin Juan Wang Hui Xiao Jia Li Xia Peng Huirong Dai Li Li |
| author_facet | Fei Li Yuping Wang Lihui Lin Juan Wang Hui Xiao Jia Li Xia Peng Huirong Dai Li Li |
| author_sort | Fei Li |
| collection | DOAJ |
| description | Exosomes are nanovesicles released by different cell types, such as dendritic cells (DCs), mast cells (MCs), and tumor cells. Exosomes of different origin play a role in antigen presentation and modulation of immune response to infectious disease. In this study, we demonstrate that mast cells and CD4+ T cells colocated in peritoneal lymph nodes from BALB/c mouse. Further, bone marrow-derived mast cells (BMMCs) constitutively release exosomes, which express CD63 and OX40L. BMMC-exosomes partially promoted the proliferation of CD4+ T cells. BMMC-exosomes significantly enhanced the differentiation of naive CD4+ T cells to Th2 cells in a surface contact method, and this ability was partly inhibited by the addition of anti-OX40L Ab. In conclusion, BMMC-exosomes promoted the proliferation and differentiation of Th2 cells via ligation of OX40L and OX40 between exosomes and T cells. This method represents a novel mechanism, in addition to direct cell surface contacts, soluble mediators, and synapses, to regulate T cell actions by BMMC-exosomes. |
| format | Article |
| id | doaj-art-49b77791b1b7459393dbf33353c05347 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-49b77791b1b7459393dbf33353c053472025-02-03T00:59:45ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/36238983623898Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 LigationFei Li0Yuping Wang1Lihui Lin2Juan Wang3Hui Xiao4Jia Li5Xia Peng6Huirong Dai7Li Li8Department of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaDepartment of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiao Tong University School, Shanghai 200080, ChinaExosomes are nanovesicles released by different cell types, such as dendritic cells (DCs), mast cells (MCs), and tumor cells. Exosomes of different origin play a role in antigen presentation and modulation of immune response to infectious disease. In this study, we demonstrate that mast cells and CD4+ T cells colocated in peritoneal lymph nodes from BALB/c mouse. Further, bone marrow-derived mast cells (BMMCs) constitutively release exosomes, which express CD63 and OX40L. BMMC-exosomes partially promoted the proliferation of CD4+ T cells. BMMC-exosomes significantly enhanced the differentiation of naive CD4+ T cells to Th2 cells in a surface contact method, and this ability was partly inhibited by the addition of anti-OX40L Ab. In conclusion, BMMC-exosomes promoted the proliferation and differentiation of Th2 cells via ligation of OX40L and OX40 between exosomes and T cells. This method represents a novel mechanism, in addition to direct cell surface contacts, soluble mediators, and synapses, to regulate T cell actions by BMMC-exosomes.http://dx.doi.org/10.1155/2016/3623898 |
| spellingShingle | Fei Li Yuping Wang Lihui Lin Juan Wang Hui Xiao Jia Li Xia Peng Huirong Dai Li Li Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation Journal of Immunology Research |
| title | Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation |
| title_full | Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation |
| title_fullStr | Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation |
| title_full_unstemmed | Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation |
| title_short | Mast Cell-Derived Exosomes Promote Th2 Cell Differentiation via OX40L-OX40 Ligation |
| title_sort | mast cell derived exosomes promote th2 cell differentiation via ox40l ox40 ligation |
| url | http://dx.doi.org/10.1155/2016/3623898 |
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