Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats
Background: There is growing interest in natural antioxidants, particularly Solanum nigrum (S. nigrum), because of their potential to protect against oxidative stress-induced organ damage and their intriguing historical use in treating various medical conditions. Aim: This pioneering study delved...
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| Format: | Article |
| Language: | English |
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Tripoli University
2025-04-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=229616 |
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| author | Taher Issa Shailabi Ahmed Saeed Kabbashi Osama Hussein Aldeeb Eman Salah Basheer Rehab Aeyad Gumaa Ebtihal Jebriel Younes Retaj Fawzi Mohammed Amar Mohamed Ismail |
| author_facet | Taher Issa Shailabi Ahmed Saeed Kabbashi Osama Hussein Aldeeb Eman Salah Basheer Rehab Aeyad Gumaa Ebtihal Jebriel Younes Retaj Fawzi Mohammed Amar Mohamed Ismail |
| author_sort | Taher Issa Shailabi |
| collection | DOAJ |
| description | Background:
There is growing interest in natural antioxidants, particularly Solanum nigrum (S. nigrum), because of their potential to protect against oxidative stress-induced organ damage and their intriguing historical use in treating various medical conditions.
Aim:
This pioneering study delved into the unique antioxidant, hepatoprotective, and renoprotective effects of S. nigrum methanolic leaf extract on carbon tetrachloride (CCl4)- induced toxicity in albino rats.
Methods:
Thirty albino rats (100–120 g) were used. The DPPH radical scavenging assay was used to evaluate the antioxidant properties of S. nigrum extract. This study examined the protective effects of 150 and 300 mg/kg leaf extract against CCl4-induced toxicity. Body weight, hematological markers, and kidney and liver functions were assessed. Histological analysis of liver and kidney tissues was performed.
Results:
The radical scavenging activity of S. nigrum was a remarkable 85.02±0.01 RSA%, compared to 66.83±0.02 RSA% for gallic acid, with IC50 values of 58.3 µg/ml and 1.22 µg/ml, respectively. S. nigrum treatment significantly decreased alanine transaminase (ALT) and aspartate transaminase (AST) activities relative to CCl4-induced toxicity, a finding of significant importance in pharmacology and toxicology.
Conclusion:
The S. nigrum methanolic extract has an IC50 value of 58.3 µg/ml, demonstrating potent natural antioxidant properties that mitigate hepatotoxicity and renotoxicity induced by CCl4. [Open Vet J 2025; 15(4.000): 1585-1592] |
| format | Article |
| id | doaj-art-49b0f7ec74a64cfc842b31bf8f15ef5c |
| institution | OA Journals |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-49b0f7ec74a64cfc842b31bf8f15ef5c2025-08-20T02:15:00ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502025-04-011541585159210.5455/OVJ.2025.v15.i4.9229616Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino ratsTaher Issa Shailabi0Ahmed Saeed Kabbashi1Osama Hussein Aldeeb2Eman Salah Basheer3Rehab Aeyad Gumaa4Ebtihal Jebriel Younes5Retaj Fawzi Mohammed6Amar Mohamed Ismail7Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, Libya Omar Al-Mukhtar University, Al-Bayda, LibyaBackground: There is growing interest in natural antioxidants, particularly Solanum nigrum (S. nigrum), because of their potential to protect against oxidative stress-induced organ damage and their intriguing historical use in treating various medical conditions. Aim: This pioneering study delved into the unique antioxidant, hepatoprotective, and renoprotective effects of S. nigrum methanolic leaf extract on carbon tetrachloride (CCl4)- induced toxicity in albino rats. Methods: Thirty albino rats (100–120 g) were used. The DPPH radical scavenging assay was used to evaluate the antioxidant properties of S. nigrum extract. This study examined the protective effects of 150 and 300 mg/kg leaf extract against CCl4-induced toxicity. Body weight, hematological markers, and kidney and liver functions were assessed. Histological analysis of liver and kidney tissues was performed. Results: The radical scavenging activity of S. nigrum was a remarkable 85.02±0.01 RSA%, compared to 66.83±0.02 RSA% for gallic acid, with IC50 values of 58.3 µg/ml and 1.22 µg/ml, respectively. S. nigrum treatment significantly decreased alanine transaminase (ALT) and aspartate transaminase (AST) activities relative to CCl4-induced toxicity, a finding of significant importance in pharmacology and toxicology. Conclusion: The S. nigrum methanolic extract has an IC50 value of 58.3 µg/ml, demonstrating potent natural antioxidant properties that mitigate hepatotoxicity and renotoxicity induced by CCl4. [Open Vet J 2025; 15(4.000): 1585-1592]http://www.ejmanager.com/fulltextpdf.php?mno=229616antioxidanthepatoprotectives. nigrum leafmethanolic extractalbino rats |
| spellingShingle | Taher Issa Shailabi Ahmed Saeed Kabbashi Osama Hussein Aldeeb Eman Salah Basheer Rehab Aeyad Gumaa Ebtihal Jebriel Younes Retaj Fawzi Mohammed Amar Mohamed Ismail Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats Open Veterinary Journal antioxidant hepatoprotective s. nigrum leaf methanolic extract albino rats |
| title | Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats |
| title_full | Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats |
| title_fullStr | Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats |
| title_full_unstemmed | Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats |
| title_short | Antioxidant and protective effects of methanolic Solanum nigrum leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats |
| title_sort | antioxidant and protective effects of methanolic solanum nigrum leaf extract against ccl4 induced hepatotoxicity and renotoxicity in albino rats |
| topic | antioxidant hepatoprotective s. nigrum leaf methanolic extract albino rats |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=229616 |
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