Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF
The effect of glycosaminoglycans (GAGs) on the proliferation of smooth muscle cells (SMC) and fibroblasts was assessed by culturing cells with or without GAGs. Porcine heparan sulphate (HS) inhibited proliferation in a dose dependent manner. At 167 μg/ml of HS this reached 88% and 72% inhibition of...
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| Format: | Article |
| Language: | English |
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Wiley
1993-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000419 |
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| author | lan E. Silber Jeanine M. Walenga Jawed Fareed Elizabeth J. Kovacs |
| author_facet | lan E. Silber Jeanine M. Walenga Jawed Fareed Elizabeth J. Kovacs |
| author_sort | lan E. Silber |
| collection | DOAJ |
| description | The effect of glycosaminoglycans (GAGs) on the proliferation of smooth muscle cells (SMC) and fibroblasts was assessed by culturing cells with or without GAGs. Porcine heparan sulphate (HS) inhibited proliferation in a dose dependent manner. At 167 μg/ml of HS this reached 88% and 72% inhibition of SMC and fibroblast growth, respectively. Pig and beef mucosal heparins also blocked proliferation, but to a lesser extent. In contrast, beef lung heparin, chondroitin sulphate, and dermatan sulphate failed to block growth factor induced proliferation. Continuous presence of HS was not required, suggesting that the inhibitory effects resulted from a direct effect on the cell rather than an interaction of the GAG with growth factors. The mechanism by which GAGs inhibit proliferation will be addressed in future studies. |
| format | Article |
| id | doaj-art-499b797d497e489aac8d3ba692df989b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-499b797d497e489aac8d3ba692df989b2025-02-03T00:59:26ZengWileyMediators of Inflammation0962-93511466-18611993-01-012429930210.1155/S0962935193000419Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGFlan E. Silber0Jeanine M. Walenga1Jawed Fareed2Elizabeth J. Kovacs3Molecular Biology Program, Neurobiology and Anatomy, Loyola University, Maywood, IL 60153, USADept of Thoracic and Cardiovascular Surgery, Neurobiology and Anatomy, Loyola University, Maywood, IL 60153, USADept of Pharmacology, Neurobiology and Anatomy, Loyola University, Maywood, IL 60153, USAMolecular Biology Program, Neurobiology and Anatomy, Loyola University, Maywood, IL 60153, USAThe effect of glycosaminoglycans (GAGs) on the proliferation of smooth muscle cells (SMC) and fibroblasts was assessed by culturing cells with or without GAGs. Porcine heparan sulphate (HS) inhibited proliferation in a dose dependent manner. At 167 μg/ml of HS this reached 88% and 72% inhibition of SMC and fibroblast growth, respectively. Pig and beef mucosal heparins also blocked proliferation, but to a lesser extent. In contrast, beef lung heparin, chondroitin sulphate, and dermatan sulphate failed to block growth factor induced proliferation. Continuous presence of HS was not required, suggesting that the inhibitory effects resulted from a direct effect on the cell rather than an interaction of the GAG with growth factors. The mechanism by which GAGs inhibit proliferation will be addressed in future studies.http://dx.doi.org/10.1155/S0962935193000419 |
| spellingShingle | lan E. Silber Jeanine M. Walenga Jawed Fareed Elizabeth J. Kovacs Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF Mediators of Inflammation |
| title | Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF |
| title_full | Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF |
| title_fullStr | Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF |
| title_full_unstemmed | Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF |
| title_short | Heparan sulphate inhibition of cell proliferation induced by TGFβ and PDGF |
| title_sort | heparan sulphate inhibition of cell proliferation induced by tgfβ and pdgf |
| url | http://dx.doi.org/10.1155/S0962935193000419 |
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