Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing
BACKGROUND: There has been a growing interest in developing an appropriate laboratory diagnostic algorithm for Clostridium difficile, mainly as a result of increases in both the number and severity of cases of C difficile infection in the past decade. A C difficile diagnostic algorithm is necessary...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2013/934945 |
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| _version_ | 1832567788804243456 |
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| author | Michelle J Alfa Shadi Sepehri |
| author_facet | Michelle J Alfa Shadi Sepehri |
| author_sort | Michelle J Alfa |
| collection | DOAJ |
| description | BACKGROUND: There has been a growing interest in developing an appropriate laboratory diagnostic algorithm for Clostridium difficile, mainly as a result of increases in both the number and severity of cases of C difficile infection in the past decade. A C difficile diagnostic algorithm is necessary because diagnostic kits, mostly for the detection of toxins A and B or glutamate dehydrogenase (GDH) antigen, are not sufficient as stand-alone assays for optimal diagnosis of C difficile infection. In addition, conventional reference methods for C difficile detection (eg, toxigenic culture and cytotoxin neutralization [CTN] assays) are not routinely practiced in diagnostic laboratory settings. |
| format | Article |
| id | doaj-art-49923059a741403681817d333a5b948e |
| institution | Kabale University |
| issn | 1712-9532 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-49923059a741403681817d333a5b948e2025-02-03T01:00:34ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95322013-01-01242899210.1155/2013/934945Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic TestingMichelle J Alfa0Shadi Sepehri1Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, CanadaDepartment of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, CanadaBACKGROUND: There has been a growing interest in developing an appropriate laboratory diagnostic algorithm for Clostridium difficile, mainly as a result of increases in both the number and severity of cases of C difficile infection in the past decade. A C difficile diagnostic algorithm is necessary because diagnostic kits, mostly for the detection of toxins A and B or glutamate dehydrogenase (GDH) antigen, are not sufficient as stand-alone assays for optimal diagnosis of C difficile infection. In addition, conventional reference methods for C difficile detection (eg, toxigenic culture and cytotoxin neutralization [CTN] assays) are not routinely practiced in diagnostic laboratory settings.http://dx.doi.org/10.1155/2013/934945 |
| spellingShingle | Michelle J Alfa Shadi Sepehri Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing |
| title_full | Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing |
| title_fullStr | Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing |
| title_full_unstemmed | Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing |
| title_short | Combination of Culture, Antigen and Toxin Detection, and Cytotoxin Neutralization Assay for Optimal Clostridium difficile Diagnostic Testing |
| title_sort | combination of culture antigen and toxin detection and cytotoxin neutralization assay for optimal clostridium difficile diagnostic testing |
| url | http://dx.doi.org/10.1155/2013/934945 |
| work_keys_str_mv | AT michellejalfa combinationofcultureantigenandtoxindetectionandcytotoxinneutralizationassayforoptimalclostridiumdifficilediagnostictesting AT shadisepehri combinationofcultureantigenandtoxindetectionandcytotoxinneutralizationassayforoptimalclostridiumdifficilediagnostictesting |