Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also,...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.211 |
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| author | C. Lobo M. Sanchez C. Garbi E. Ferrer M.J. Martinez-Iñigo J.L. Allende C. Martinez L. Casasas R. Alonso A. Gibello M. Martin |
| author_facet | C. Lobo M. Sanchez C. Garbi E. Ferrer M.J. Martinez-Iñigo J.L. Allende C. Martinez L. Casasas R. Alonso A. Gibello M. Martin |
| author_sort | C. Lobo |
| collection | DOAJ |
| description | Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation. |
| format | Article |
| id | doaj-art-49850b6c99594a4d86e57c0f9816cfc1 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-49850b6c99594a4d86e57c0f9816cfc12025-02-03T01:31:14ZengWileyThe Scientific World Journal1537-744X2002-01-0121361136810.1100/tsw.2002.211Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and ModelingC. Lobo0M. Sanchez1C. Garbi2E. Ferrer3M.J. Martinez-Iñigo4J.L. Allende5C. Martinez6L. Casasas7R. Alonso8A. Gibello9M. Martin10Inst. Madrileño de Investigacion Agraria y Alimentaria. Apt. 127, 28800 Madrid, SpainFacultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainFacultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainFacultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainInst. Madrileño de Investigacion Agraria y Alimentaria. Apt. 127, 28800 Madrid, SpainFacultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainETSIA y ETSII, Universidad Politécnica, 28040 Madrid, SpainETSIA y ETSII, Universidad Politécnica, 28040 Madrid, SpainETSIA y ETSII, Universidad Politécnica, 28040 Madrid, SpainFacultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainFacultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainBased on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation.http://dx.doi.org/10.1100/tsw.2002.211 |
| spellingShingle | C. Lobo M. Sanchez C. Garbi E. Ferrer M.J. Martinez-Iñigo J.L. Allende C. Martinez L. Casasas R. Alonso A. Gibello M. Martin Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling The Scientific World Journal |
| title | Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling |
| title_full | Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling |
| title_fullStr | Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling |
| title_full_unstemmed | Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling |
| title_short | Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling |
| title_sort | immobilized native bacteria as a tool for bioremediation of soils and waters implementation and modeling |
| url | http://dx.doi.org/10.1100/tsw.2002.211 |
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