Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of <i>Aspergillus flavipes</i> HN4-13
Emodin is an anthraquinone compound known for its diverse biological activities, including anti-tumor and anti-inflammatory effects, making it highly applicable in the fields of biology and medicine. The production of emodin using microorganisms represents a sustainable and environmentally friendly...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/4/174 |
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| Summary: | Emodin is an anthraquinone compound known for its diverse biological activities, including anti-tumor and anti-inflammatory effects, making it highly applicable in the fields of biology and medicine. The production of emodin using microorganisms represents a sustainable and environmentally friendly approach. A marine-derived <i>Aspergillus flavipes</i> HN4-13 was found to produce emodin, but the yield was too low for industrial production. To develop a high-yielding emodin-producing strain, we established the high-through detection and screening methods of alkaline coloration and deep-well plant culture, enabling the effective selection of high-yielding strains. Following ARTP mutagenesis of the wild strain <i>A. flavipes</i> HN4-13, the resulting mutant strain, M1440, exhibited an increased emodin yield of 124.6 ± 4.95 mg/L. Furthermore, the production of the emodin was enhanced by the exogenous addition of metal ions Mn<sup>2+</sup> to the medium. Specifically, the addition of 3 mM Mn<sup>2+</sup> resulted in a 133.2% increase in emodin production, with the highest yield reaching 178.6 ± 7.80 mg/L. |
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| ISSN: | 1660-3397 |