A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development
Alternative splicing (AS) is a crucial post-transcriptional regulatory mechanism that contributes to proteome complexity and versatility in different plant species. However, detailed AS exploration in diploid cotton during fiber development has not been reported. In this study, we comparatively anal...
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2024-10-01
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| author | Jianfeng Hao Xingpeng Wen Yuxian Zhu |
| author_facet | Jianfeng Hao Xingpeng Wen Yuxian Zhu |
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| description | Alternative splicing (AS) is a crucial post-transcriptional regulatory mechanism that contributes to proteome complexity and versatility in different plant species. However, detailed AS exploration in diploid cotton during fiber development has not been reported. In this study, we comparatively analyzed <i>G. arboreum</i> and <i>G. raimondii</i> AS events during fiber development using transcriptome data and identified 9690 and 7617 AS events that were distributed in 6483 and 4859 genes, respectively. <i>G. arboreum</i> had more AS genes and AS events than <i>G. raimondii</i>, and most AS genes were distributed at both ends of all 13 chromosomes in both diploid cotton species. Four major AS types, including IR, SE, A3SS, and A5SS, were all experimentally validated through RT-PCR assays. <i>G. arboreum</i> and <i>G. raimondii</i> had only 1888 AS genes in common, accounting for one-third and one-half of the total number of AS genes, respectively. Furthermore, we found a lysine-specific demethylase coding gene with a different AS mechanism in <i>G. arboreum</i> and <i>G. raimondii</i>, in which AS isoforms lacked part of a key conserved domain. Our findings may provide new directions for the discovery of functional genes involved in cotton species differentiation. |
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| spelling | doaj-art-49703614e1a24150bbb8e4f8e7b514b32025-08-20T01:47:37ZengMDPI AGPlants2223-77472024-10-011319281610.3390/plants13192816A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber DevelopmentJianfeng Hao0Xingpeng Wen1Yuxian Zhu2College of Life Sciences, Wuhan University, Wuhan 430072, ChinaCollege of Life Sciences, Wuhan University, Wuhan 430072, ChinaCollege of Life Sciences, Wuhan University, Wuhan 430072, ChinaAlternative splicing (AS) is a crucial post-transcriptional regulatory mechanism that contributes to proteome complexity and versatility in different plant species. However, detailed AS exploration in diploid cotton during fiber development has not been reported. In this study, we comparatively analyzed <i>G. arboreum</i> and <i>G. raimondii</i> AS events during fiber development using transcriptome data and identified 9690 and 7617 AS events that were distributed in 6483 and 4859 genes, respectively. <i>G. arboreum</i> had more AS genes and AS events than <i>G. raimondii</i>, and most AS genes were distributed at both ends of all 13 chromosomes in both diploid cotton species. Four major AS types, including IR, SE, A3SS, and A5SS, were all experimentally validated through RT-PCR assays. <i>G. arboreum</i> and <i>G. raimondii</i> had only 1888 AS genes in common, accounting for one-third and one-half of the total number of AS genes, respectively. Furthermore, we found a lysine-specific demethylase coding gene with a different AS mechanism in <i>G. arboreum</i> and <i>G. raimondii</i>, in which AS isoforms lacked part of a key conserved domain. Our findings may provide new directions for the discovery of functional genes involved in cotton species differentiation.https://www.mdpi.com/2223-7747/13/19/2816alternative splicing<i>G. arboreum</i><i>G. raimondii</i>transcriptomefiber |
| spellingShingle | Jianfeng Hao Xingpeng Wen Yuxian Zhu A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development Plants alternative splicing <i>G. arboreum</i> <i>G. raimondii</i> transcriptome fiber |
| title | A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development |
| title_full | A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development |
| title_fullStr | A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development |
| title_full_unstemmed | A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development |
| title_short | A Genome-Wide Alternative Splicing Analysis of <i>Gossypium arboreum</i> and <i>Gossypium raimondii</i> During Fiber Development |
| title_sort | genome wide alternative splicing analysis of i gossypium arboreum i and i gossypium raimondii i during fiber development |
| topic | alternative splicing <i>G. arboreum</i> <i>G. raimondii</i> transcriptome fiber |
| url | https://www.mdpi.com/2223-7747/13/19/2816 |
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