Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal
Drought and salinity are major environmental constraints that severely limit crop production, particularly in arid and semi-arid zones. We investigated the genetic diversity of arbuscular mycorrhizal fungi isolated from the rhizosphere of <i>Vachellia seyal</i> in three different soils f...
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2025-01-01
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| author | Anicet Georges Bruno Manga Godar Sene André Amakobo Diatta Tahir Abdoulaye Diop Gérard Barroso Diederik van Tuinen |
| author_facet | Anicet Georges Bruno Manga Godar Sene André Amakobo Diatta Tahir Abdoulaye Diop Gérard Barroso Diederik van Tuinen |
| author_sort | Anicet Georges Bruno Manga |
| collection | DOAJ |
| description | Drought and salinity are major environmental constraints that severely limit crop production, particularly in arid and semi-arid zones. We investigated the genetic diversity of arbuscular mycorrhizal fungi isolated from the rhizosphere of <i>Vachellia seyal</i> in three different soils from Senegal with varying levels of salinity. Soil and root samples were collected from under <i>V. seyal</i> and in the vicinity of the trees. After DNA extraction, nested PCR, and sequencing of the large subunit region of the rRNA gene, different phylotypes from rhizospheric soils, roots, and spores were compared by phylogeny in order to investigate the role of salinity in arbuscular fungal diversity. This study revealed several unidentified arbuscular fungi and a particularly high host specificity in <i>V. seyal</i> roots. The vast majority of operational taxonomic units (OTUs) isolated in this study had no homologous sequences in the databases. |
| format | Article |
| id | doaj-art-4957076c83704b399db070cbc2e4f730 |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Microbiology Research |
| spelling | doaj-art-4957076c83704b399db070cbc2e4f7302025-01-24T13:41:45ZengMDPI AGMicrobiology Research2036-74812025-01-011611910.3390/microbiolres16010019Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in SenegalAnicet Georges Bruno Manga0Godar Sene1André Amakobo Diatta2Tahir Abdoulaye Diop3Gérard Barroso4Diederik van Tuinen5Département Productions Végétales et Agronomie, UFR des Sciences Agronomiques, de l’Aquaculture et des Technologies Alimentaires (S2ATA), Universite Gaston Berger, Saint Louis BP 234, SenegalLaboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Département de Biologie Végétale, Université Cheikh Anta Diop (UCAD), Dakar BP 5005, SenegalDépartement Productions Végétales et Agronomie, UFR des Sciences Agronomiques, de l’Aquaculture et des Technologies Alimentaires (S2ATA), Universite Gaston Berger, Saint Louis BP 234, SenegalUFR des Sciences Agricoles et de l’Alimentation (SAA), Université Amadou Makhtar Mbow, Diamniadio BP 45927, SenegalUF Biologie, University of Bordeaux, INRAE, MycSA, F-33882 Villenave d’Ornon, FranceAgroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, FranceDrought and salinity are major environmental constraints that severely limit crop production, particularly in arid and semi-arid zones. We investigated the genetic diversity of arbuscular mycorrhizal fungi isolated from the rhizosphere of <i>Vachellia seyal</i> in three different soils from Senegal with varying levels of salinity. Soil and root samples were collected from under <i>V. seyal</i> and in the vicinity of the trees. After DNA extraction, nested PCR, and sequencing of the large subunit region of the rRNA gene, different phylotypes from rhizospheric soils, roots, and spores were compared by phylogeny in order to investigate the role of salinity in arbuscular fungal diversity. This study revealed several unidentified arbuscular fungi and a particularly high host specificity in <i>V. seyal</i> roots. The vast majority of operational taxonomic units (OTUs) isolated in this study had no homologous sequences in the databases.https://www.mdpi.com/2036-7481/16/1/19arbuscular mycorrhizal fungi<i>Vachellia seyal</i>salinityphylogeny |
| spellingShingle | Anicet Georges Bruno Manga Godar Sene André Amakobo Diatta Tahir Abdoulaye Diop Gérard Barroso Diederik van Tuinen Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal Microbiology Research arbuscular mycorrhizal fungi <i>Vachellia seyal</i> salinity phylogeny |
| title | Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal |
| title_full | Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal |
| title_fullStr | Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal |
| title_full_unstemmed | Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal |
| title_short | Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal |
| title_sort | molecular diversity of arbuscular mycorrhizal fungi associated with the rhizosphere of i vachellia seyal i del from selected saline soils in senegal |
| topic | arbuscular mycorrhizal fungi <i>Vachellia seyal</i> salinity phylogeny |
| url | https://www.mdpi.com/2036-7481/16/1/19 |
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