Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study
The effects of montelukast against methotrexate-induced liver damage were investigated. 35 Wistar albino female rats were divided into 5 groups as follows: group I: control; group II: montelukast (ML); group III: methotrexate (Mtx); group IV: montelukast treatment after methotrexate application (Mtx...
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| Format: | Article |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/987508 |
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| author | Evren Kose Hilal Irmak Sapmaz Ediz Sarihan Nigar Vardi Yusuf Turkoz Nihat Ekinci |
| author_facet | Evren Kose Hilal Irmak Sapmaz Ediz Sarihan Nigar Vardi Yusuf Turkoz Nihat Ekinci |
| author_sort | Evren Kose |
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| description | The effects of montelukast against methotrexate-induced liver damage were investigated. 35 Wistar albino female rats were divided into 5 groups as follows: group I: control; group II: montelukast (ML); group III: methotrexate (Mtx); group IV: montelukast treatment after methotrexate application (Mtx + ML); group V: montelukast treatment before methotrexate application (ML + Mtx). At the end of the experiment, the liver tissues of rats were removed. Malondialdehyde (MDA), myeloperoxidase (MPO), and reduced glutathione levels were determined from liver tissues. In addition, the liver tissues were examined histologically. MDA and MPO levels of Mtx group were significantly increased when compared to control group. In Mtx + ML group, these parameters were decreased as compared to Mtx group. Mtx injection exhibited major histological alterations such as eosinophilic staining and swelling of hepatocytes. The glycogen storage in hepatocytes was observed as decreased by periodic acid schiff staining in Mtx group as compared to controls. ML treatment did not completely ameliorate the lesions and milder degenerative alterations as loss of the glycogen content was still present. It was showed that montelukast treatment after methotrexate application could reduce methotrexate-induced experimental liver damage. |
| format | Article |
| id | doaj-art-4954711e1dc346d0a6ae16f27422d4f8 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4954711e1dc346d0a6ae16f27422d4f82025-02-03T05:48:39ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/987508987508Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological StudyEvren Kose0Hilal Irmak Sapmaz1Ediz Sarihan2Nigar Vardi3Yusuf Turkoz4Nihat Ekinci5Department of Anatomy, School of Medicine, İnonu University Medical Faculty, 44280 Malatya, TurkeyDepartment of Anatomy, School of Medicine, İnonu University Medical Faculty, 44280 Malatya, TurkeyDepartment of Emergency, Inonu University, 44280 Malatya, TurkeyDepartment of Histology-Embryology, Inonu University, 44280 Malatya, TurkeyDepartment of Biochemistry, Inonu University, 44280 Malatya, TurkeyDepartment of Anatomy, School of Medicine, İnonu University Medical Faculty, 44280 Malatya, TurkeyThe effects of montelukast against methotrexate-induced liver damage were investigated. 35 Wistar albino female rats were divided into 5 groups as follows: group I: control; group II: montelukast (ML); group III: methotrexate (Mtx); group IV: montelukast treatment after methotrexate application (Mtx + ML); group V: montelukast treatment before methotrexate application (ML + Mtx). At the end of the experiment, the liver tissues of rats were removed. Malondialdehyde (MDA), myeloperoxidase (MPO), and reduced glutathione levels were determined from liver tissues. In addition, the liver tissues were examined histologically. MDA and MPO levels of Mtx group were significantly increased when compared to control group. In Mtx + ML group, these parameters were decreased as compared to Mtx group. Mtx injection exhibited major histological alterations such as eosinophilic staining and swelling of hepatocytes. The glycogen storage in hepatocytes was observed as decreased by periodic acid schiff staining in Mtx group as compared to controls. ML treatment did not completely ameliorate the lesions and milder degenerative alterations as loss of the glycogen content was still present. It was showed that montelukast treatment after methotrexate application could reduce methotrexate-induced experimental liver damage.http://dx.doi.org/10.1100/2012/987508 |
| spellingShingle | Evren Kose Hilal Irmak Sapmaz Ediz Sarihan Nigar Vardi Yusuf Turkoz Nihat Ekinci Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study The Scientific World Journal |
| title | Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study |
| title_full | Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study |
| title_fullStr | Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study |
| title_full_unstemmed | Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study |
| title_short | Beneficial Effects of Montelukast Against Methotrexate-Induced Liver Toxicity: A Biochemical and Histological Study |
| title_sort | beneficial effects of montelukast against methotrexate induced liver toxicity a biochemical and histological study |
| url | http://dx.doi.org/10.1100/2012/987508 |
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