HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients
Abstract Background SARS-CoV-2 infection affects both lipid metabolism and lung function. The severity of the disease has been associated with reduced levels of both high-density lipoprotein (HDL) and low-density lipoprotein cholesterol. Despite the crucial role that these nanoparticles play in SARS...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Lipids in Health and Disease |
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| Online Access: | https://doi.org/10.1186/s12944-024-02381-w |
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| author | Floran Begue Bryan Veeren Philippe Rondeau Aline-Marie Florence Simon Jamard Philippe Montravers Sébastien Tanaka Olivier Meilhac on behalf of French COVID Study Group |
| author_facet | Floran Begue Bryan Veeren Philippe Rondeau Aline-Marie Florence Simon Jamard Philippe Montravers Sébastien Tanaka Olivier Meilhac on behalf of French COVID Study Group |
| author_sort | Floran Begue |
| collection | DOAJ |
| description | Abstract Background SARS-CoV-2 infection affects both lipid metabolism and lung function. The severity of the disease has been associated with reduced levels of both high-density lipoprotein (HDL) and low-density lipoprotein cholesterol. Despite the crucial role that these nanoparticles play in SARS-CoV-2 infection, few studies have examined their structure during COVID-19 beyond HDL quantity. The study aimed to assess apolipoprotein levels in COVID-19 patients who either survived or died following ICU admission. In addition, ICU survivors and non-survivors were compared for HDL particle size and proteome. Methods Between February and April 2020, our study enrolled 37 COVID-19 patients upon their intensive care unit admission. Among them, 18 survived the disease, while 19 succumbed to it. We used mass spectrometry to assess plasma levels of 14 apolipoproteins and LCAT. Additionally, we analyzed HDL subpopulation distribution by utilizing native polyacrylamide gel electrophoresis. HDL particles were isolated from both surviving and non-surviving patients using ultracentrifugation, followed by characterization of their proteomes with NanoLC-MS/MS. Results Plasma apolipoproteins, including Apo A-II, Apo Cs (I, II, III), Apo H, Apo J, Apo M, and LCAT, were decreased in patients who did not survive COVID-19. However, no alterations were noted in the distribution of HDL subpopulations in relation to mortality. HDL composition was further altered based on mortality, displaying a decline in Apo H and paraoxonase 3. Conclusion In conclusion, we have shown an alteration in plasma apolipoproteins and HDL composition between surviving COVID-19 patients and non-survivors. Some markers, such as Apo H, are more predictive than baseline lipid concentrations such as HDL-C. These markers appear to provide a more accurate indication of mortality during COVID-19 compared with baseline lipid concentrations such as HDL-C. |
| format | Article |
| id | doaj-art-4947f700b2a644d9991e2baa29d59ffe |
| institution | Kabale University |
| issn | 1476-511X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj-art-4947f700b2a644d9991e2baa29d59ffe2025-02-02T12:40:40ZengBMCLipids in Health and Disease1476-511X2025-01-0124111310.1186/s12944-024-02381-wHDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patientsFloran Begue0Bryan Veeren1Philippe Rondeau2Aline-Marie Florence3Simon Jamard4Philippe Montravers5Sébastien Tanaka6Olivier Meilhac7on behalf of French COVID Study GroupUniversité de La Réunion, INSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI)Université de La Réunion, INSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI)Université de La Réunion, INSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI)INSERM IAME UMR 1137, Université Paris CitéDepartment of Infectious Disease, University Hospital of ToursDepartment of Anesthesiology and Critical Care Medicine, Assistance, AP-HP, Bichat-Claude Bernard HospitalUniversité de La Réunion, INSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI)Université de La Réunion, INSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI)Abstract Background SARS-CoV-2 infection affects both lipid metabolism and lung function. The severity of the disease has been associated with reduced levels of both high-density lipoprotein (HDL) and low-density lipoprotein cholesterol. Despite the crucial role that these nanoparticles play in SARS-CoV-2 infection, few studies have examined their structure during COVID-19 beyond HDL quantity. The study aimed to assess apolipoprotein levels in COVID-19 patients who either survived or died following ICU admission. In addition, ICU survivors and non-survivors were compared for HDL particle size and proteome. Methods Between February and April 2020, our study enrolled 37 COVID-19 patients upon their intensive care unit admission. Among them, 18 survived the disease, while 19 succumbed to it. We used mass spectrometry to assess plasma levels of 14 apolipoproteins and LCAT. Additionally, we analyzed HDL subpopulation distribution by utilizing native polyacrylamide gel electrophoresis. HDL particles were isolated from both surviving and non-surviving patients using ultracentrifugation, followed by characterization of their proteomes with NanoLC-MS/MS. Results Plasma apolipoproteins, including Apo A-II, Apo Cs (I, II, III), Apo H, Apo J, Apo M, and LCAT, were decreased in patients who did not survive COVID-19. However, no alterations were noted in the distribution of HDL subpopulations in relation to mortality. HDL composition was further altered based on mortality, displaying a decline in Apo H and paraoxonase 3. Conclusion In conclusion, we have shown an alteration in plasma apolipoproteins and HDL composition between surviving COVID-19 patients and non-survivors. Some markers, such as Apo H, are more predictive than baseline lipid concentrations such as HDL-C. These markers appear to provide a more accurate indication of mortality during COVID-19 compared with baseline lipid concentrations such as HDL-C.https://doi.org/10.1186/s12944-024-02381-wCOVID-19MortalityHDL particleApolipoproteomeApolipoproteinProteomic |
| spellingShingle | Floran Begue Bryan Veeren Philippe Rondeau Aline-Marie Florence Simon Jamard Philippe Montravers Sébastien Tanaka Olivier Meilhac on behalf of French COVID Study Group HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients Lipids in Health and Disease COVID-19 Mortality HDL particle Apolipoproteome Apolipoprotein Proteomic |
| title | HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients |
| title_full | HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients |
| title_fullStr | HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients |
| title_full_unstemmed | HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients |
| title_short | HDL proteome and apolipoproteins concentrations in severe ICU COVID-19 patients |
| title_sort | hdl proteome and apolipoproteins concentrations in severe icu covid 19 patients |
| topic | COVID-19 Mortality HDL particle Apolipoproteome Apolipoprotein Proteomic |
| url | https://doi.org/10.1186/s12944-024-02381-w |
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